Abstract This study aimed to identify differential circular RNA (circRNA) in the plasma exosomes of patients with lung adenocarcinoma (LUAD) using high‐throughput sequencing. First, were isolated an exosome isolation kit and confirmed by Western blotting, transmission electron microscopy, NanoSight Assay. Subsequently, circRNA expression profiles screened sequencing fluorescence quantitative real‐time polymerase chain reaction (qRT‐PCR) Sanger Finally, circRNA‐miRNA‐mRNA network was...

Background Colorectal cancer (CRC) is the third most common and it a worldwide challenge. Neutrophil-to-lymphocyte ratio (NLR) platelet-to-lymphocyte (PLR) had been suggested as markers of CRC, but role monocyte-to-lymphocyte (MLR) in CRC patients before surgery chemotherapy unclear. The study aimed to compare diagnosis prognosis value MLR, NLR, PLR CRC. Methods A retrospective was conducted on 783 with histologically confirmed colorectal between 2015 2017 Fujian Medical University Union...

Background This study aimed to investigate the correlation between quantitative retinal vascular parameters such as central arteriolar equivalent (CRAE) and fractal dimension (D(f)), cardiovascular risk factors in Chinese Han population residing islands of southeast China. Methodology/Principle Findings In this cross-sectional study, fundus photographs were collected semi-automated analysis software was used analyze vessel diameters dimensions. Cardiovascular relevant medical history, blood...

Abstract Exosomal microRNAs (miRNAs) have great potentials as a novel biomarker to predict lung cancer. We applied miRNA microarray identify aberrantly expressed serum exosomal miRNAs candidate biomarkers for patients with adenocarcinoma (LUAD). Compared the normal control, 31 were found be upregulated and 29 downregulated in of LUAD respectively. Then, 10 dysregulated expression levels further validated via qRT‐polymerase chain reaction. Notably, miR‐7977 was highest miR‐98‐3p lowest LUAD,...

Lactate dehydrogenase C (LDHC) is a kind of cancer-testis antigen (CTA) that has been reported to be biomarker for diagnosis, efficacy evaluation, and recurrence monitoring lung adenocarcinoma (LUAD). This study aims assess the value LDHC in peptide-based vaccines LUAD immunotherapy. The recombinant protein was purified its effect on PC9 cells evaluated by wound healing assay, Transwell invasion, migration assay. Ten HLA-A2-restricted LDHC-derived peptides were predicted synthesized,...

Myeloperoxidase (MPO) anti-neutrophil cytoplasm autoantibody (ANCA)-associated vasculitis commonly causes life-threatening pulmonary alveolar hemorrhage or fibrosis. Only a limited number of candidate gene variants have been explored, but hitherto, are not widely confirmed. In the present study, we investigated importance energy homeostasis associated (Enho) mutations and adropin deficiency in development MPO-ANCA lung injury.We analyzed peripheral blood mononuclear cells from 152 unrelated...

The incidence of type 2 diabetes mellitus is increasing worldwide, and it might partly cause metabolic disorder susceptibility in patients' offspring through epigenetic modification. However, the underlying mechanisms remain largely unclear. Recent studies have shown a potential link between deoxyribonucleic acid methylation paternal sperm to offspring, so this article focuses on whether whole-genome profiles spermatozoa patients changed.

Abstract Background DARS2 was overexpressed in multiple tumor types, but the biological role of lung adenocarcinoma (LUAD) have not been elucidated. Methods Firstly, expression LUAD explored using The Cancer Genome Atlas (TCGA). Then, qRT‐PCR and Western blot were performed to confirm LUAD. Next, Cox regression Kaplan–Meier methods utilized evaluate whether can affect overall survival. relationships between clinicopathological characteristics investigated by TCGA database. Moreover, we Gene...

Background The severity, symptoms, and outcome of COVID-19 is thought to be closely linked how the virus enters host cells. This process involves key roles angiotensin-converting enzyme 2 (ACE2) Tyrosine protein kinase receptor UFO (AXL) receptors. However, there limited research on circulating levels ACE2 AXL their implications in COVID-19. Methods A control group 71 uninfected individuals was also included study. According Guidance for Corona Virus Disease 2019 (10th edition), a cohort 358...

FANCA mutations have been detected in a variety of cancers and found to be pro-carcinogenic. However, no functional studies identified regarding the involvement occurrence immune response LUAD.

Aim: To investigate whether plasma miR-19a can serve as a biomarker for esophageal squamous cell carcinoma (ESCC) diagnosis and prognosis. Materials & methods: Plasma samples from 89 ESCC, 45 benign lesion patients 80 healthy controls were subjected to RT-qPCR analyses miR-19a. In addition, 30 collected before after surgery the same analyses. Results: was significantly increased in ESCC compared with controls. The sensitivity of early stages 68.09%. Combination cytokeratin 19 fragment 21–1...

To describe the relationship of plasma apelin levels with blood pressure in a coastal Chinese population.This cross-sectional study included total 1031 subjects from areas China. One-way analysis variance (ANOVA) and linear trend test, Pearson's correlation analysis, as well multivariate regression were used to evaluate association between pressure.Plasma dropped increasing quartiles systolic (SBP), diastolic (DBP), mean arterial (MABP) (all P<0.001). SBP, DBP, MABP values decreased...

Abstract The outbreak of carbapenem-resistant Klebsiella pneumoniae is a serious public health problem, especially in the neonatal intensive care unit (NICU). Fifteen K. strains were isolated from 7 neonates during June 3 to 28, 2017 an NICU. Antimicrobial susceptibility was determined by Vitek 2 system and microbroth dilution method. Multilocus sequence typing (MLST) pulsed-field gel electrophoresis (PFGE) used analyze genetic relatedness isolates. Whole-genome sequencing gene function...

Background: The peroxiredoxin (PRDX) protein family is involved in cancer cell invasion and metastasis, but its prognostic value lung remain elusive. Methods: In this report, we accessed the overall survival (OS) of each individual PRDX mRNA expression through Kaplan–Meier plotter (KM plotter) database, which updated gene data information include a total 1,926 patients. Results: Our results indicated that PRDX1 PRDX2 expressions were associated with improved OS all patients especially...

Recently, we have demonstrated that PRSS1 mutations cause ectopic trypsinogen activation and thereby result in type 1 autoimmune pancreatitis (AIP). However, the molecules involved inducing obliterative vasculitis perineural inflammation pancreas are not well-described. The present study applied whole-exome sequencing (WES) to determine underlying etiology revealed novel missense splice region variants, CALCB c.88T>C (p.Ser30Pro) IR [1]-mutants, 2 of 3 families 26 unrelated patients with...

Background. Rhodococcus equi is one of the most important causes zoonotic infections from grazing animals. It poses a particular risk to immunocompromised individuals, including those who are undergoing long-term immunosuppressive therapy. Case presentation. We report case infection in 65-year-old man with medical history diabetes, hypertension, and Adult Still’s Disease, currently taking hormone The non-human immunodeficiency virus (HIV)-infected patient had blood, lung tissue, sputum...

Apelin activity plays a role in regulating blood pressure. This study explored the relationship between single nucleotide polymorphisms (SNPs) gene (APLN) with hypertension and central retinal artery equivalent (CRAE) stenosis coastal Chinese population. All subjects answered an epidemiological survey for demographic disease characteristics. levels were determined three APLN SNPs, rs56204867, rs3115757, rs3761581, evaluated. CRAE was measured using fundus photography. significantly lower...

Many studies showed an association between dietary salt intake, blood pressure and increased CVD risk. The potential reason may be related to vascular structural functional changes, through alterations in endothelial function. central retinal arteriolar equivalent urinary albumin reflected dysfunction different part of the body. sodium-creatinine ratio causal urine specimens could represent 24-h sodium intake estimate intake. excretion was estimated by ratio. Urinary albumin-creatinine...

Background Lung cancer is the most prevalent and deadliest worldwide. The present study aims to determine prognosis value of low expression long non-coding RNAs (lncRNAs) in LUAD. Methods RNA-seq data clinical information were downloaded from Cancer Genome Atlas (TCGA) data-base. Dysregulated genes between LUAD paracancerous tissue screened by GeneSpringGX. Prognostic lncRNAs which expressed filtrated Ualcan, then further verified through TCGA database. association clinicopathological...

Coronavirus disease 2019 (COVID-19) is associated with immune dysregulation and cytokine storm. It essential to explore the response characteristics of peripheral circulation in COVID-19 patients reveal pathogenesis predict progression. In this study, levels total immunoglobulins (IgG, IgM, IgA), complement (C3, C4)，lymphocyte subsets (CD3

The roles that adventitious roots play in flooding tolerance have been well-studied willows, while which of the maximum height and abundance willows is more important for their not well known. In this study, we analyzed effects on Salix cavaleriei by comparing dead live along a gradient from 0 to 180 cm Lake Erhai, China. results showed willow mortality increased drastically when water depth suffered exceeded 100 cm. Live developed produced them higher trees compared with however, pest...