The coronavirus disease 2019 (Covid-19), which caused respiratory problems in many patients worldwide, led to more than 5 million deaths by the end of 2021. Experienced symptoms vary from mild severe illness. Understanding infection severity reach a better prognosis could be useful clinics, and one study area fulfill piece this biological puzzle is metabolomics. metabolite profile and/or levels being monitored can help predict phenotype properties. Therefore, evaluated plasma metabolomes 110...

Abstract The neurodegeneration that occurs in methylmalonic acidemia is proposed to be associated with impairment of mitochondrial oxidative metabolism resulting from methylmalonate (MMA) accumulation. present study evaluated the effects MMA on oxygen consumption by isolated rat brain mitochondria presence NADH‐linked substrates (α‐ketoglutarate, citrate, isocitrate, glutamate, malate, and pyruvate). Respiration supported either glutamate or plus malate was significantly inhibited (1–10 mM),...

Diacetyl, methylglyoxal, and glyoxal are α-dicarbonyl catabolites prone to nucleophilic additions of amino groups proteins nucleobases, thereby triggering adverse biological responses. Because their electrophilicity, in aqueous medium, they exist a phosphate-catalyzed dynamic equilibrium with hydrate forms. Diacetyl methylglyoxal can be attacked by peroxynitrite (k(2) ≈ 1.0 × 10(4) M(-1) s(-1) k(2) 10(5) s(-1), respectively), potent nucleophile oxidant, yielding the acetyl radical from...

Occupational exposure to potentially toxic elements (PTEs) is a concerning reality of informal workers engaged in the jewelry production chain that can lead adverse health effects. In this study, untargeted proteomic and metabolomic analyses were employed assess impact these exposures on workers' exposome Limeira city, São Paulo state, Brazil. PTE levels (Cr, Mn, Ni, Cu, Zn, As, Cd, Sn, Sb, Hg, Pb) determined blood, performed for saliva samples (n = 26), plasma 145) using ultra-high...

Composite cathode materials based on nickel, such as NiFe, NiMo, NiMoCd, NiS, and , are of considerable practical interest possible electrocatalysts for hydrogen production in alkaline water electrolyzers. This work describes the development a new composite material Ni FeS (referred to Ni/NiFeS) that shows high electrocatalytic activity toward evolution reaction. Ni/NiFeS coatings were obtained by codeposition particles previously electrodeposited mild steel substrate. The evaluation this...

Abstract Several conditions have been used in the coupling reaction of stepwise SPPS at elevated temperature (SPPS‐ET), but we elected following as our first choice: 2.5‐fold molar excess 0.04–0.08 M Boc or Fmoc‐amino acid derivative, equimolar amount DIC/HOBt (1:1) TBTU/DIPEA (1:3), 25% DMSO/toluene, 60 °C, conventional heating. In this study, aimed to further examine enantiomerization under such condition and study applicability protocols microwave‐SPPS, peptides containing L ‐Ser, ‐His,...

Abstract Background Schizophrenia (SCZ) is a severe mental disorder of multifactorial origin in which the role amino acids relatively unknown. Based on hypothesis that there an imbalance acid levels individuals with SCZ compared healthy controls (HCs), aim this study was to identify 20 proteinogenic involved protein synthesis. Methods This included 43 sex‐ and age‐matched individuals: HCs 23 diagnosed using Diagnostic Statistical Manual Mental Disorders criteria. Using proton nuclear...

Methylglyoxal is an α-oxoaldehyde putatively produced in excess from triose phosphates, aminoacetone, and acetone some disorders, particularly diabetes. Here, we investigate the nucleophilic addition of ONOO−, known as a potent oxidant nucleophile, to methylglyoxal, yielding acetyl radical intermediate ultimately formate acetate ions. The rate ONOO− decay presence methylglyoxal [k2,app = (1.0 ± 0.1) × 103 M−1 s−1; k2 ≈ 1.0 105 s−1] at pH 7.2 25 °C was found be faster than that reported with...

Behçet's disease (BD) is a relapsing, multisystem and inflammatory condition characterized by systemic vasculitis of small large vessels. Although the etiopathogenesis BD remains unknown, immune-mediated mechanisms play major role in development disease. patients present leukocyte infiltration mucocutaneous lesions as well neutrophil hyperactivation. In contrast to neutrophils, whose involvement pathogenesis has been extensively studied, biology monocytes during less known. this study, we...

The detection of plant hormones and growth regulators is great interest for many biological studies especially in the determination metabolites related to plan differentiation. In this work, we propose a simple method based on capillary electrophoresis (CE) separation different classes such as auxins, cytokinins, gibberelic acid abscisic acid. CE with UV was used analytical conditions were follows: phosphate buffer 25 mmol L-1, all measurements pH 12 or 2.5, by hydrodynamic injection 5 s at...

COUPLED TO MASS SPECTROMETRY (CE-MS): TWENTY YEARS OF DEVELOPMENT.CE-MS has been increasingly used for analysis of a vast array compounds.This article reviews the different electrophoretic modes, interfaces and mass analyzers that are commonly in CE-MS coupling, as well technique advantages performance characteristics.A large compilation applications is also presented.Therefore, this review both guide beginners collection key references people who familiar to technique.Furthermore, first...

Diacetyl is a flavoring that imparts buttery flavor to foods, but the use or exposure diacetyl has been related some diseases. We investigated effect of oral intake in male and female C57/Bl mice. performed target metabolomics assay using ultraperformance liquid chromatography paired with triple quadrupole mass spectrometry (UPLC-MS/MS) for determination quantification plasmatic metabolites. observed alterations metabolites present urea tricarboxylic acid (TCA) cycles. Peroxynitrite levels...

Diacetyl, like other α-dicarbonyl compounds, is reportedly cytotoxic and genotoxic. A food cigarette contaminant, it related with alcohol hepatotoxicity lung disease. Peroxynitrite a potent oxidant formed in vivo by the diffusion-controlled reaction of superoxide radical anion nitric oxide, which able to form adducts carbon dioxide carbonyl compounds. Here, we investigate nucleophilic addition peroxynitrite diacetyl forming acetyl radicals, whose molecular oxygen leads acetate. shown react...

Recentemente nosso grupo demostrou experimentalmente que a hipótese de produção radical acetil em meio tamponado no sistema diacetil/peroxinitrito era possível.Diacetil é um flavorizante alimentos, cigarros e bebidas.O peroxinitrito encontrado na mitocôndria, certas condições como processo infeção humanos, concentração aumenta significativamente.Em sistemas biológicos, radicais podem facilmente modificar estrutura atividade ácidos nucleicos, proteínas outras biomoléculas, causando...

Composting is a sustainable approach to manage animal and vegetal waste generated in the Fundação Parque Zoológico de São Paulo. The resulting compost often used ZOO's premises as an organic fertilizer for production of vegetables, which further feed animals. composting product provides many forms mineral also amino acids (AA) that are absorbed by plants nutrients. Since most absorb only slightly or not at all UV wavelengths, we developed method determination AA agricultural interest...

OBJECTIVE: To compare the incidence of Covid-19 symptoms between informal home-based workers and a control group to assess association these cases with blood elements concentrations other relevant risk factors for Sars-Cov-2 infection. METHODS: Welders chemically exposed potentially toxic (PTEs) (n = 26) participants 25) answered questionnaires on adherence social distancing signs disease five months during pandemic. After follow-up, serology tests were performed subsample 12 20...

Acetylation alters several protein properties including molecular weight, stability, enzymatic activity, protein-protein interactions, and other biological functions. Our previous findings demonstrating that diacetyl/peroxynitrite can acetylate L-lysine, L-histidine, albumin in vitro led us to investigate whether diacetyl-treated rats suffer acetylation as well.Wistar were administered diacetyl daily for four weeks, after which they sacrificed, their lung proteins extracted be analysed by...

Cystinuria is an autosomal recessive genetic disorder characterized by abnormal intestinal and renal tubular transport of L-cystine as well L-lysine, L-arginine L-ornithine. This leads to excessive urinary excretion amino acids, with the formation kidney stones caused low solubility in urine. In this study, analytical method for simultaneous determination these four acids urine capillary electrophoresis coupled electrospray ionization mass spectrometry (CE-ESI-MS) was developed validated....