A5027235707- Polyomavirus and related diseases
- Antenna Design and Analysis
- Full-Duplex Wireless Communications
- Neurobiology and Insect Physiology Research
- Neonatal Respiratory Health Research
- Insect and Arachnid Ecology and Behavior
- Congenital heart defects research
- Medical Imaging and Pathology Studies
- Immunotherapy and Immune Responses
- Developmental Biology and Gene Regulation
- IL-33, ST2, and ILC Pathways
- Dermatological and COVID-19 studies
- Cancer and Skin Lesions
- Vascular Tumors and Angiosarcomas
- Sarcoma Diagnosis and Treatment
- Hemiptera Insect Studies
- Animal Behavior and Reproduction
- Cutaneous Melanoma Detection and Management
- COVID-19 Clinical Research Studies
- Asthma and respiratory diseases
- Hedgehog Signaling Pathway Studies
- Parvovirus B19 Infection Studies
- Connective tissue disorders research
- Plant Virus Research Studies
- Infectious Diseases and Mycology
Michigan Medicine
2017-2025
Michigan United
2011-2024
University of Michigan–Ann Arbor
2014-2024
Pulmonary and Critical Care Associates
2021
University of Pittsburgh Medical Center
2021
Beaumont Health
2021
Wayne State University
2007-2014
Yale University
2013
University of Exeter
2010
Significance Human mutations in SOX9 lead to several congenital disorders, including campomelic dysplasia. Babies born with this condition often die of respiratory distress; however, defects lung development have thus far not been reported mouse models. Here, we report that epithelial-specific deletion Sox9 leads developmental abnormalities the during branching morphogenesis. We demonstrate plays multiple roles epithelium, balancing proliferation and differentiation regulating extracellular...
We investigated the role of histone methyltransferase Ezh2 in tangential migration mouse precerebellar pontine nuclei, main relay between neocortex and cerebellum. By counteracting sonic hedgehog pathway, represses Netrin1 dorsal hindbrain, which allows normal neuron migration. In mutants, ectopic derepression results abnormal supernumerary nuclei integrating brain circuitry. Moreover, intrinsic topographic organization according to rostrocaudal progenitor origin is maintained throughout...
Abstract Purpose: Merkel cell carcinoma (MCC) is an aggressive cutaneous neuroendocrine that can be divided into two classes: virus-positive (VP) MCC, associated with oncogenic polyomavirus (MCPyV); and virus-negative (VN) photodamage. Experimental Design: We classified 346 MCC tumors from 300 patients for MCPyV using a combination of IHC, ISH, qPCR assays. In subset tumors, we profiled mutation status expression cancer-relevant genes. molecular profiling results were correlated...
Wnt/β-catenin (βcat) signaling is critical for adrenal homeostasis. To elucidate how Wnt/βcat elicits homeostatic maintenance of the cortex, we characterized identity adrenocortical Wnt-responsive population. We find that cells consist sonic hedgehog (Shh)-producing progenitors and differentiated, steroidogenic zona glomerulosa, but not fasciculata rarely are actively proliferating. determine potential direct inhibitory effects βcat on fasciculata-associated steroidogenesis, used mouse ATCL7...
Early-life respiratory virus infections have been correlated with enhanced development of childhood asthma. In particular, significant numbers syncytial (RSV)-hospitalized infants go on to develop lung disease. It has suggested that early-life viral may lead altered or repair negatively impacts function later in life. Our data demonstrate RSV infection modifies structure, leading decreased function. At 5 wk postneonatal infection, defects are observed baseline pulmonary test (PFT) parameters...
Organogenesis requires the differentiation and integration of distinct populations cells to form a functional organ. In kidney, reciprocal interactions between ureter nephrogenic mesenchyme are required for organ formation. Additionally, stromal also necessary proper development this Much remains be understood regarding origin cortical pathways involved in their formation function. By generating triple mutants Hox10 paralogous group genes, we demonstrate that genes play critical role...
Hox genes are required for proper anteroposterior axial patterning and the development of several organ systems. Here, we show that all three Hox5 paralogous play redundant roles in developing lung. Hoxa5;Hoxb5;Hoxc5 triple-mutant embryos develop severely hypoplastic lungs with reduced branching proximal-distal defects. exclusively expressed lung mesoderm; however, defects observed both mesenchyme endodermally derived epithelium, demonstrating act to regulate mesodermal-epithelial crosstalk...
Preferentially expressed antigen in melanoma (PRAME) is an immunohistochemical biomarker that diffusely most cutaneous melanomas and negative benign nevi. Histologically challenging dermal melanocytic neoplasms, such as cellular blue nevi (CBN) deep penetrating (DPN), soft tissue tumors with differentiation, clear cell sarcoma perivascular epithelioid tumor, may resemble primary or metastatic melanoma. PRAME immunohistochemistry (IHC) was applied to archived formalin-fixed, paraffin-embedded...
ABSTRACT Interstitial stromal cells play critical roles in muscle development, regeneration and repair we have previously reported that Hoxa11 Hoxd11 are expressed the interstitial of muscles attached to zeugopod, crucial for proper embryonic patterning these muscles. Hoxa11eGFP expression continues a subset through adult stages. The induction Hoxa11-CreERT2-mediated lineage reporting (Hoxa11iTom) at stages mouse results only cells. However, Hoxa11iTom+ progressively contribute fibers...
Follicular dendritic cell sarcoma (FDCS) is a rare neoplasm requiring high index of suspicion, especially on small biopsies. Smooth muscle myosin heavy chain (SMMHC) common immunohistochemical (IHC) stain that has been reported to mark normal nodal follicular cells (FDCs). We hypothesize SMMHC can be sensitive marker for FDCS and aim compare its performance with established markers FDCS. The archive large academic center was searched cases Clinical features, including age, sex, site at...
Differential enlargement of hind (T3) legs represents one the hallmarks insect evolution. However, actual mechanism(s) responsible are yet to be determined. To address this issue, we have now studied molecular basis T3 leg in Oncopeltus fasciatus (milkweed bug) and Acheta domesticus (house cricket). In Oncopeltus, tibia displays a moderate increase size, whereas Acheta, femur, tibia, tarsus all greatly enlarged. Here, show that hox gene Ultrabithorax (Ubx) is expressed enlarged segments...
In insects, forewings and hindwings usually have different shapes, sizes, color patterns. A variety of RNAi experiments across insect species shown that the hox gene Ultrabithorax (Ubx) is necessary to promote hindwing identity. However, it remains unclear whether Ubx sufficient confer fate insects. Here, we address this question by over-expressing in butterfly Bicyclus anynana using a heat-shock promoter. whole-body over-expression during embryonic larvae development led body plan changes...
Significance Mammalian Hox genes are important for limb development. Posterior abdominal B ( AbdB ) groups Hox9 – Hox13 required establishment of the proximodistal axis. In addition, control onset Hand2 expression in posterior forelimb, and HoxA/D responsible initiation maintenances Sonic Hedgehog Shh ). this study, we generated Hox5 triple mutants, resulting embryos with severe forelimb anterior patterning defects. We found that proteins interact promyelocytic leukemia zinc finger to...
Despite significant advances in our understanding of pancreatic endocrine cell development, the function mesodermal niche this process is poorly understood. Here we report a novel role for Hox6 genes organogenesis. are expressed exclusively mesoderm developing pancreas. Genetic loss all three paralogs (Hoxa6, Hoxb6, Hoxc6) leads to dramatic endoderm-derived cells including insulin-secreting beta cells, as well mild delays and disruptions pancreas branching exocrine differentiation....
<ns4:p><ns4:italic>Hox</ns4:italic>proteins are a deeply conserved group of transcription factors originally defined for their critical roles in governing segmental identity along the antero-posterior (AP) axis in<ns4:italic>Drosophila</ns4:italic>. Over last 30 years, numerous data generated evolutionarily diverse taxa have clearly shown that changes expression patterns these genes closely associated with regionalization AP axis, suggesting that<ns4:italic>Hox</ns4:italic>genes played role...
Diagnosis of spindle cell/sarcomatoid melanoma may be challenging due to frequent loss expression melanocytic marker(s) and histomorphologic resemblance various mesenchymal tumours, particularly malignant peripheral nerve sheath tumour (MPNST). Overexpression PReferentially expressed Antigen in MElanoma (PRAME) supports a diagnosis when evaluating tumours. PRAME MPNST other cutaneous sarcomatoid neoplasms, however, has not been well characterised. We aimed determine the utility immunostain...
PReferentially expressed Antigen in MElanoma (PRAME) is a cancer-testis antigen upregulated multitude of human neoplasms, while minimally normal adult tissues except germ cells.1 The PRAME immunostain has gained interest recent years, particularly the realm diagnostically challenging melanocytic lesions. Large cohorts have shown diffuse expression 80%–83% all melanomas; on other hand, benign nevi showed little to absent expression.2-6 Unlike most types melanoma, however, only minority...