A5064284841- Ocular Infections and Treatments
- Ocular Surface and Contact Lens
- Asthma and respiratory diseases
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Air Quality and Health Impacts
- NF-κB Signaling Pathways
- Retinal and Optic Conditions
- Corneal Surgery and Treatments
- Acne and Rosacea Treatments and Effects
- Sarcoidosis and Beryllium Toxicity Research
- Caveolin-1 and cellular processes
- Sinusitis and nasal conditions
- Antimicrobial Resistance in Staphylococcus
- Allergic Rhinitis and Sensitization
- Retinal Diseases and Treatments
- Eosinophilic Esophagitis
- Proteoglycans and glycosaminoglycans research
- Antioxidant Activity and Oxidative Stress
- Air Quality Monitoring and Forecasting
- Skin Protection and Aging
- Ocular Disorders and Treatments
- Sphingolipid Metabolism and Signaling
Victor Chang Cardiac Research Institute
2024
Macquarie University
2019-2020
UNSW Sydney
2005-2017
Brien Holden Vision Institute
2010-2016
The objectives of this study were to determine whether a synergistic effect could be obtained in vitro between bovine lactoferricin (B-LFcin) and antibiotics against Pseudomonas aeruginosa Staphylococcus aureus isolates from ocular infections, evaluate the use B-LFcin as an adjunct antibiotic treatment corneal infection vivo.Chequerboard time-kill assays performed investigate combined effects conventional antibiotics, including ciprofloxacin, ceftazidime gentamicin, 17 strains P. (8) S. (9)...
Abstract Background and objective Exposure to airborne particulate matter ( PM ) may promote development of childhood asthma trigger acute exacerbations existing via injury airway epithelial cells AEC ). Methods We compared the response ambient particulates with median aerodynamic diameters <10 μm or <2.5 from Sydney metropolitan region S ydney 10 2.5), traffic‐derived exhaust stack a motorway tunnel inert carbon black as control. Results strongly stimulated messenger RNA expression...
Staphylococcus is a leading cause of the potentially blinding disease microbial keratitis. Even with use antibiotic therapy, host inflammatory response continues to damage cornea, which may lead blindness. Manipulation help improve patient outcome from this devastating disease. We aim understand contribution aureus infection. A S. keratitis mouse model was developed in both C57BL/6 and BALB/c mice using two different strains (8325‐4 Staph 38). Twenty‐four hours postinfection, were killed...
We investigated the role of interleukin-33 (IL-33) in airway inflammation an experimental model acute exacerbation chronic asthma, which reproduces many features human disease. Systemically sensitized female BALB/c mice were challenged with a low mass concentration aerosolized ovalbumin for 4 weeks to induce asthmatic and then received single moderate-level challenge trigger simulating exacerbation. The inflammatory response expression cytokines activation markers by alveolar macrophages...
To determine whether interleukin-6 (IL-6) plays a protective role in Staphylococcus aureus keratitis gene knockout (gko) mouse model and to IL-6 may be used as therapy modulate host responses control bacterial infection, thereby reducing scarring.The eyes of gko mice wild-type were challenged topically with S. examined at 24 hours after infection. Keratitis was clinically histologically. Bacterial polymorphonuclear leukocytes (PMNs) enumerated, cytokine chemokine levels determined by ELISA....
Abstract Pseudomonas is one of the leading causes contact lens-related microbial keratitis. Despite use antibiotics, host inflammatory response continues to cause damage cornea, which may lead blindness. CXCR2-binding chemokines have been implicated in pathogenesis keratitis, and exact role this receptor remains be elucidated. Corneas CXCR2 knockout wild-type mice (Cmkar 2−/− Cmkar 2+/+) were scratched, 2 × 106 CFU/mL 6294 or 6206 was added corneas. Twenty-four hours postinfection, killed,...
Respiratory viral infections are the most common trigger of acute exacerbations in patients with allergic asthma. The anti-viral response airway epithelial cells (AEC) may be impaired asthmatics, while cytokines produced by AEC drive inflammatory response. We investigated whether cultured presence Th2 associated an environment exhibited altered responses to double-stranded RNA, a virus-like stimulus.We undertook preliminary studies using MLE-12 cell line derived from mouse distal respiratory...
Childhood exposure to environmental particulates increases the risk of development asthma. The underlying mechanisms might include oxidant injury airway epithelial cells (AEC). We investigated ability ambient contribute sensitization via airways, and thus pathogenesis childhood To do so, we devised a novel model in which weanling BALB/c mice were exposed both particulate pollutants ovalbumin for respiratory tract, followed by chronic inhalational challenge with low mass concentration...
Airway epithelial cells (AEC) exhibit a pro-inflammatory phenotype in patients with allergic asthma. We examined the effect of an cytokine environment on response AEC to rhinovirus (RV), most common trigger acute exacerbations Calu-3 cells, well-differentiated human line, were cultured or without T-helper type 2 cytokines interleukin (IL)-4 and IL-13, then stimulated toll-like receptor (TLR) 3 agonist (poly I:C, dsRNA) TLR7 (imiquimod), infected RV 16. Expression antiviral mediators, viral...
Pseudomonas aeruginosa keratitis is one of the most destructive diseases cornea. The host response to this infection critical outcome, and regulated by cytokines produced in ocular tissue. In study, we assessed relative contribution cornea inflammatory whole eye gain a better understanding regulatory processes environment during localized corneal infection. C57BL/6 mice were challenged topical application P. wounded corneas. Corneas eyes harvested 24 h post‐challenge bacterial numbers,...
We sought to determine whether human corneal limbal epithelial cells (HCLE) differ in their physiological response and production of inflammatory mediators during exposure two multipurpose disinfecting solutions that only four excipients.HCLE were exposed dilutions (1%-20%) OPTI-FREE Express RepleniSH for 2, 6, or 18 h. Cell numbers measured using CyQuant metabolic activity assays. Morphology, viability, apoptotic changes examined by confocal microscopy after staining with Calcein...
Interleukin‐4 (IL‐4) has previously been implicated in a protective response to Staphylococcus aureus corneal infection. Consequently, the specific role of IL‐4 during S. infection was investigated using gene knockout mice. The eyes IL‐4−/− mice and wild‐type were challenged topically with examined at 24 h post‐infection. Keratitis clinically histologically. Bacterial polymorphonuclear leucocytes (PMN) numbers enumerated cytokine chemokine levels determined by enzyme‐linked immunosorbent...
Sarcoidosis has often been termed an "immune paradox" as there is peripheral anergy to common recall antigens despite pronounced TH1-dominant inflammation at disease sites, such the lung, with up-regulation of interferon γ, IL-27 and transcription factors. Peripheral blood may reflect anergic state, while exhaled breath condensate (EBC) analysis offer insights into lung disease.A cross-sectional study was conducted investigate expression TH1 cytokines factors (IFNγ, T-bet) in and/or EBC...
There are few alternatives to glucocorticosteroids for treatment of asthma. We assessed the activity a novel protein drug designated ISU201, extracellular domain human cell surface BST2, stabilised by fusion with Fc region IgG, in mouse models mild chronic asthma and an acute exacerbation The ability ISU201 suppress airway inflammation remodelling was compared that dexamethasone. Female BALB/c mice were systemically sensitised ovalbumin, then received controlled low-level challenge...
Glucocorticoids are commonly used for treating asthma and its exacerbations but have well-recognised adverse effects not always effective. Few alternative treatments exist. Using a murine model of an acute exacerbation asthma, we assessed the ability ISU201, novel protein drug, to suppress inflammatory response when administered after induction exacerbation. Sensitised mice were chronically challenged with low mass concentration aerosolised ovalbumin, then received single moderate-level...
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive disease with few treatment options and poor survivability. In this work we sought to characterise metabolic adaptions gemcitabine (GEMC)-based chemotherapy exposure discover new therapeutic targets for improving efficacy. We show that GEMC resistance (GEMR) upregulates de novo lipogenesis in Panc1 MiaPaCa2 cells through increased activity expression of acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS) stearoyl-CoA...