- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Microbial infections and disease research
- Diet and metabolism studies
- Muscle metabolism and nutrition
- Biosensors and Analytical Detection
- Sports Performance and Training
- Exercise and Physiological Responses
- Viral gastroenteritis research and epidemiology
- Rabies epidemiology and control
- Animal Disease Management and Epidemiology
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Vector-Borne Animal Diseases
- Adipose Tissue and Metabolism
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Bacterial Identification and Susceptibility Testing
- Animal Virus Infections Studies
- Thermoregulation and physiological responses
- Streptococcal Infections and Treatments
- Infectious Diseases and Tuberculosis
- Pharmacology and Obesity Treatment
- Virology and Viral Diseases
- Brucella: diagnosis, epidemiology, treatment
Animal and Plant Health Agency
2011-2023
Rhode Island College
2021-2022
Providence College
2021
ARUP Laboratories (United States)
2009-2014
Guildford Hospital
2014
University of Surrey
2014
Springfield College
2006-2013
Central Connecticut State University
2011-2013
University of Lynchburg
2011-2013
Drury University
2011-2013
ABSTRACT Several reverse transcription-PCR (RT-PCR) methods have been reported for the detection of rabies and rabies-related viruses. These invariably involve multiple transfers nucleic acids between different tubes, with risk contamination leading to production false-positive results. Here we describe a single, closed-tube, nonnested RT-PCR TaqMan technology that distinguishes classical virus (genotype 1) European bat lyssaviruses 1 2 (genotypes 5 6) in real time. The assay is rapid,...
Sawyer, JC, Wood, RJ, Davidson, PW, Collins, SM, Matthews, TD, Gregory, and Paolone, VJ. Effects of a short-term carbohydrate-restricted diet on strength power performance. J Strength Cond Res 27(8): 2255–2262, 2013—The purpose the study was to examine effects switching from habitual (CRD) performance in trained men (n = 16) women 15). Subjects performed handgrip dynamometry, vertical jump, 1RM bench press back squat, maximum-repetition press, 30-second Wingate anaerobic cycling test after...
Abstract The incidence of Mycobacterium bovis , the causative agent bovine tuberculosis, has been increasing in UK cattle herds resulting substantial economic losses. European badger ( Meles meles ) is implicated as a wildlife reservoir infection. One likely route transmission to through exposure infected urine and faeces. relative importance environment remains unknown, part due lack information on distribution magnitude environmental reservoirs. Here we identify potential infection...
Graphical abstract Mycobacterium bovis is a slow-growing (16–20 h generation time), Gram-positive and acid-fast bacterium member of the tuberculosis complex pathogen group (MTBC). They are characterized by complex, protective cell wall containing mycolic acids. The MTBCs causative agents (TB). Following initial infection, subsequent pathological changes, progress infection depend on interplay between host defence mechanisms mycobacterial virulence factors balance immunologic responses...
Preserving fat-free mass (FFM) during weight loss is important in older adults. The purpose was to examine a low-fat diet (LFD) versus carbohydrate-restricted (CRD) with and without progressive resistance exercise (PRE) on preservation of FFM men metabolic syndrome.A total 42 (59±7 years) were matched [body index (BMI)] randomized LFD, LFD&PRE, CRD, CRD&PRE. PRE groups performed supervised strength training three times per week. Body weight, composition, syndrome criteria, measured at...
Bovine tuberculosis (bTB), caused by Mycobacterium bovis, represents a major animal health issue. In the United Kingdom and Republic of Ireland, European badgers (Meles meles) have been shown to act as reservoir M. bovis infection, hindering eradication bTB in livestock. The availability suitable diagnostic assays, particularly those that may be applied "trap-side" setting, would facilitate implementation wider range disease control strategies. Here we evaluate Dual Path Platform (DPP) VetTB...
A real‐time PCR was developed to detect Coxiella burnetii (the cause of Q fever) in ruminant placentas and aborted fetuses. Primer probe sets previously for human tissue studies were used target the insertion sequence IS 1111 gene C . The assay highly sensitive, with a limit detection 10 copies template, theoretically equating single bacterium, did not cross‐react panel other bacteria. To determine sensitivity on field samples submitted diagnosis abortion, results using compared com 1 assay....
The bacterium Coxiella burnetii, which has a wide host range, causes Q fever. Infection with C. burnetii can cause abortions, stillbirth, and the delivery of weak offspring in ruminants. infection is zoonotic, human beings it chronic, potentially fatal disease. Real-time polymerase chain reaction (PCR) increasingly being used to detect organism aid diagnosis both animal cases. Many different real-time PCR methods, target genes, have been described. To assess comparability assays use European...
Advances in the diagnosis of Mycobacterium bovis infection wildlife hosts may benefit development sustainable approaches to management bovine tuberculosis cattle. In present study, three laboratories from two different countries participated a validation trial evaluate reliability and reproducibility real time PCR assay detection quantification M. environmental samples. The sample panels consisted negative badger faeces spiked with dilution series BCG Pasteur field samples badgers unknown...
ABSTRACT The incidence of Mycobacterium bovis , the causative agent bovine tuberculosis, in cattle herds United Kingdom is increasing, resulting substantial economic losses. European badger ( Meles meles ) implicated as a wildlife reservoir and subject control measures aimed at reducing infection populations. Understanding epidemiology M. populations essential for directing interventions understanding disease spread; however, accurate diagnosis live animals challenging currently uses...
Staphylococcus pseudintermedius is a commensal and opportunistic pathogen of dogs. It mainly implicated in canine pyoderma, as well other suppurative conditions Although bacterial culture routinely used for clinical diagnosis, molecular methods are required to accurately identify differentiate S. from members the intermedius group. These methods, owing largely their cost, not easy implement nonspecialized laboratories or veterinary practices. In current study, loop-mediated isothermal...
Summary Reasons for performing study Infection with S treptococcus equi subspecies ( . ) is endemic in the UK A proportion of horses serve as long‐term carriers and act a reservoir infection. Detection these persistently infected difficult using standard culture techniques owing to lack sensitivity overgrowth by contaminating bacteria. In addition, differentiation this causative bacterium from closely related zooepidemicus has made development reliable accurate diagnostic tests difficult....
Journal Article HYDATID DISEASE TRANSMISSION IN CALIFORNIA: A STUDY OF THE BASQUE CONNECTION Get access F. P. ARAUJO, ARAUJO Search for other works by this author on: Oxford Academic PubMed Google Scholar C. W. SCHWABE, SCHWABE J. SAWYER, SAWYER 2Animal and Plant Health Inspection Service of the U. S. Department Agriculture G. DAVIS 3Department Anthropology, University CaliforniaDavis American Epidemiology, Volume 102, Issue 4, October 1975, Pages 291–302,...
Brucellosis is a bacterial zoonotic disease of major global importance. Natural hosts for Brucella species include animals economic significance, such as cattle and small ruminants. Controlling brucellosis in natural by high-throughput serological testing followed the slaughter seropositive helps to prevent transmission. This study aimed convert an existing competitive enzyme-linked immunosorbent assay (cELISA), used serodiagnosis ruminants, two electrochemiluminescence (ECL) immunoassays on...