A5072656505- Phytochemicals and Antioxidant Activities
- Carcinogens and Genotoxicity Assessment
- Genomics, phytochemicals, and oxidative stress
- Cancer-related Molecular Pathways
- Head and Neck Cancer Studies
- Cancer therapeutics and mechanisms
- Biochemical Analysis and Sensing Techniques
- Food Quality and Safety Studies
- Oral Health Pathology and Treatment
- Bioactive Compounds and Antitumor Agents
- Toxic Organic Pollutants Impact
- DNA Repair Mechanisms
- Gut microbiota and health
- Tea Polyphenols and Effects
- Glutathione Transferases and Polymorphisms
- Selenium in Biological Systems
- Plant Gene Expression Analysis
- Sulfur Compounds in Biology
- Bioactive natural compounds
- Effects and risks of endocrine disrupting chemicals
- Dye analysis and toxicity
- Nitric Oxide and Endothelin Effects
- DNA and Nucleic Acid Chemistry
- Polyamine Metabolism and Applications
- Lipid metabolism and biosynthesis
Penn State Milton S. Hershey Medical Center
2011-2024
Pennsylvania State University
2015-2024
Colorado State University
2019
Cancer Institute (WIA)
2007-2013
St. John's University
2005-2006
<div>Abstract<p>While tobacco smoking is a risk factor in the development of oral squamous cell carcinoma (OSCC), only fraction smokers develop disease. Compelling evidence shows that microbial community composition associated with carcinogenesis, suggesting microbiome may play role cancer smokers. We previously showed black raspberry (BRB) protects against OSCC induced by constituent dibenzo[def,p]chrysene (DBP) via alteration genetic and epigenetic markers manner consistent its...
<p>Figure S2: The growth curves of two Akkermansia muciniphila strains exposed to BRB and DBP.</p>
<p>Figure S1: The ASV richness was higher in mouse cecal samples compared to saliva samples.</p>
Worldwide, cancers of the oral cavity and pharynx comprise sixth most common malignancies. Histologically, more than 90% are squamous cell carcinoma (SCC). Epidemiologic data strongly support role exogenous factors such as tobacco, alcohol, human papilloma virus infection major causative agents. Avoidance risk has only been partially successful, survival rates have not improved despite advances in therapeutic approaches. Therefore, new or approaches to prevention and/or early detection...
We previously reported that dibenzo[a,l]pyrene (DB[a,l]P), the most potent known environmental carcinogen among polycyclic aromatic hydrocarbons (PAH) congeners, is carcinogenic in oral tissues of mice. have now developed a new mouse model which employs application fjord region diol epoxide, (±)-anti-11,12-dihydroxy-13,14-epoxy-11,12,13,14-tetrahydrodibenzo[a,l]pyrene (DB[a,l]PDE), metabolite tobacco smoke constituent DB[a,l]P, and we show its specific induction squamous cell carcinoma...
Tobacco smoking is one of the leading causes for oral cancer. Dibenzo[a,l]pyrene (DB[a,l]P), an environmental pollutant and a tobacco smoke constituent, most carcinogenic polycyclic aromatic hydrocarbon (PAH) tested to date in several animal models (target organs: skin, lung, ovary, mammary tissues). We have recently demonstrated that DB[a,l]P also capable inducing cancer mice; however, its metabolic activation ultimate genotoxic metabolite dibenzo[a,l]pyrene-11,12-dihydrodiol-13,14-epoxide...
Effects of black raspberry (BRB) extract and protocatechuic acid (PCA) on DNA adduct formation mutagenesis induced by metabolites dibenzo[a,l]pyrene (DBP) were investigated in rat oral fibroblasts. The DBP metabolites, (±)-anti-11,12-dihydroxy-11,12,-dihydrodibenzo[a,l]pyrene (DBP-diol) 11,12-dihydroxy-13,14-epoxy-11,12,13,14-tetrahydrodibenzo[a,l]pyrene (DBPDE) dose-dependent adducts mutations. DBPDE was considerably more potent, whereas the parent compound had no significant effect....
To provide insights into the cause of e-cigarette (e-cig) associated lung injury, we examined effects propylene glycol (PG) and glycerol (G), two common solvent carriers used to deliver nicotine/flavor, on markers oxidative stress inflammation in female B6C3F1 mice which had been successfully tobacco smoke (TS)-induced carcinogenesis. Mice exposed air TS were as negative positive controls, respectively. Using LC-MS/MS, showed that PG/G alone, absence nicotine, significantly increased levels...
Genetic and epigenetic alterations observed at end stage OSCC formation could be considered as a consequence of cancer development thus changes in normal or premalignant tissues which had been exposed to oral carcinogens such Dibenzo[def,p]chrysene (DBP) may better serve predictive biomarkers disease development. Many types DNA damage can induce occur early the absence evident morphological abnormalities. Therefore we used ERRBS generate genome-scale, single-base resolution methylomes from...
Abstract Most known chemopreventive agents including certain selenium compounds suppress the activation of nuclear factor κB (NF-κB), but mechanisms remain largely elusive. Toward this end, we initially showed that inhibition NF-κB DNA binding by benzyl selenocyanate (BSC) and 1,4-phenylenebis(methylene)selenocyanate (p-XSC) was reversed addition DTT; suggests formation DTT-reducible selenium-sulfur bonds between moieties cysteine residues in (p50) protein. Furthermore, inhibitory effect...
Abstract We previously reported that the environmental pollutant and tobacco smoke constituent dibenzo[def,p]chrysene (DBP) induced DNA damage, altered methylation oral squamous cell carcinoma (OSCC) in mice. In present study, we showed 5% dietary black raspberry (BRB) significantly reduced (P &lt; 0.05) levels of DBP-DNA adducts mouse cavity with comparable effect to those its constitutes. Thus, only BRB was selected examine if aberrant by DBP can be BRB. Using comparative genome-wide...
Abstract We previously showed that metabolic activation of the environmental and tobacco smoke constituent dibenzo[a,l]pyrene (DB[a,l]P) to its active fjord region diol epoxide (DB[a,l]PDE) is required induce DNA damage, mutagenesis, squamous cell carcinoma (SCC) in mouse oral cavity. In contrast procarcinogens, which were employed SCC, DB[a,l]PDE does not require exert biological effects, thus, this study was initiated examine, for first time, whether black raspberry powder (BRB) inhibits...
We were the first to demonstrate that direct application of environmental pollutant and tobacco smoke constituent dibenzo[a,l]pyrene (DB[a,l]P) into oral cavity mice induced squamous cell carcinoma (SCC) in tissues but not tongue; however, mechanisms can account for varied carcinogenicity remain be determined. Furthermore, we also showed only dA adducts, dG adducts mutagenic activity DB[a,l]P vivo. In this study, initially focused on DB[a,l]P-induced genotoxic effects both tongue tissues....
Dibenzo[def,p]chrysene (DBP), a representative example of the class polycyclic aromatic hydrocarbon (PAH), is known to induce tumors in multiple organ sites including ovary, lung, mammary glands, and oral cavity rodents. The goal this study was test hypothesis that levels DBP its metabolites reach retain for an extended time target organs as well capacity these metabolize carcinogen active can damage DNA may account tissue selective tumorigenicity. Therefore, we used radiolabeled [3H]...
Immunotherapy targeting program cell death protein 1 (PD-1) in addition to chemotherapy has improved the survival of triple-negative breast cancer (TNBC) patients. However, development resistance and toxicity remain significant problems. Using translationally relevant 4T1 mouse model TNBC, we report here that dietary administration phytochemical quercetin enhanced antitumor action Cyclophosphamide, a cytotoxic drug with immunogenic effects is part combination used TNBC. We observed favorably...
3‑({4‑[4‑(Acridin‑9‑ylamino)phenylthio]phenyl}(3‑hydroxypropyl)amino)propan‑1‑ol (CK0403) is a sulfur‑containing 9‑anilinoacridine analogue of amsacrine and was found to be more potent than its 2-({4-[4-(acridin-9-ylamino)phenylthio]phenyl}(2‑hydroxyethyl)amino)ethan‑1‑ol (CK0402) in the inhibition topoisomerase II‑catalyzed decatenation reaction. A previous study by our group reported that CK0402 effective against numerous breast cancer cell lines, combination with herceptin enhanced...
Cigarette smoke is a rich source of free radicals that can promote oxidative stress and carcinogenesis, including head neck squamous cell carcinoma (HNSCC) development; importantly, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) 8-iso-prostaglandin F2α (8-isoprostane) are biomarkers stress. Several mechanisms, the antioxidant properties black raspberry (BRB), account for their chemopreventive effects. In present clinical trial, we tested hypothesis BRB administration reduces levels in buccal...
Tobacco smoking and human papillomavirus infection are established etiological agents in the development of head neck squamous cell carcinoma (HNSCC). The incidence mortality HNSCC higher men than women. To provide biochemical basis for sex differences, we tested hypothesis that carcinogen treatment using dibenzo[def,p]chrysene, which is an environmental pollutant tobacco smoke constituent, absence or presence mouse results significantly levels DNA damage oral cavity male female mice....
A series of sulfur-containing 9-anilinoacridines related to amsacrine were synthesized and evaluated for their anticancer potential. Among the compounds, both diol-containing 2a 3, most cytotoxic sulfide against V-79 cells in vitro (IC90 = 2.1 μM 1.9 μM, respectively). non-alkyl-substituted compounds (7−9), with electron-donating substitution para (7 9) more than electron-withdrawing nitro-substituted compound 8. The limited SAR suggested importance hydroxyl functionality along its location...
In a series of previous studies we reported that black raspberry (BRB) powder inhibits dibenzo[a,l]pyrene (DBP)-induced DNA damage, mutagenesis, and oral squamous cell carcinoma (OSCC) development in mice. the present study, using human leukoplakia (MSK-Leuk1) (SCC1483) cells, tested hypothesis BRB extract (BRBE) will enhance synthesis glutathione (GSH) turn increase GSH conjugation fjord-region DBP diol epoxide (DBPDE) derived from leading to inhibition DBP-induced damage. The syntheses...