A5043754868- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Wheat and Barley Genetics and Pathology
- Plant and fungal interactions
- Plant Disease Resistance and Genetics
- Marine and coastal ecosystems
- Aquatic Ecosystems and Phytoplankton Dynamics
- Carcinogens and Genotoxicity Assessment
- Pesticide Residue Analysis and Safety
- Plant-Microbe Interactions and Immunity
- Identification and Quantification in Food
- Agriculture, Plant Science, Crop Management
- Allergic Rhinitis and Sensitization
- Biocrusts and Microbial Ecology
- Pharmaceutical and Antibiotic Environmental Impacts
- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Molecular Biology Techniques and Applications
- Contact Dermatitis and Allergies
- Fermentation and Sensory Analysis
- Microplastics and Plastic Pollution
- Isotope Analysis in Ecology
- Fungal Biology and Applications
- Analytical chemistry methods development
- Food Allergy and Anaphylaxis Research
University of Mons
2024-2025
Assiut University
2016-2024
Ghent University
2018-2024
Ghent University Hospital
2023
Sohag University
2022
Theodor Bilharz Research Institute
2021
Hacettepe University
2017-2019
Public Health Department
2019
BOKU University
2016-2017
Abstract BACKGROUND The present study aimed to investigate the occurrence of multiple toxic fungal and bacterial metabolites in 156 animal feed ( n = 77) maize 79) samples collected from three regions Upper Egypt. target analytes were quantified using ‘dilute shoot’ approach, followed by a liquid chromatography tandem mass spectrometry analysis. RESULTS In total, 115 detected both matrices, including regulated mycotoxins European Union, addition modified forms such as...
Microplastics (MPs) contaminate the global marine and terrestrial ecosystems. This environmental contamination can enter food chain, with MPs found in drinking water, fishery products other categories. Consequently, ingestion is considered main route of human exposure to MPs. Concerns exist on potential act as vectors for pathogenic bacteria, possibly threatening microbial safety. Starting from current evidence foodborne pathogens associated MPs, we review state knowledge putative role...
Fusarium graminearum can cause Giberella Ear Rot (GER) and seedling blight in maize, resulting major yield losses. Besides GER, the infected grains are consequently contaminated with multiple mycotoxins of F. graminearum. Zearalenone trichothecenes, such as deoxynivalenol its acetylated forms, among associated infection maize. In current work, we explored effect endophytic fungal genera Epicoccum Sordaria, to control comparative trials Piriformospora spp., an elusive genus. Furthermore,...
This study was conducted to investigate the natural co-occurrence of 295 fungal and bacterial metabolites in 28 samples dried date palm fruits collected from different shops distributed Assiut Governorate, Upper Egypt 2016. Extraction quantification target analytes were done using "dilute shoot" approach followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. In total, 30 toxic detected. Among these metabolites, 4 types ochratoxins including ochratoxin type A B...
This survey was conducted to investigate the contamination by multiple mycotoxins, aflatoxins (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), zearalenone (ZEA) in 61 samples of maize 17 commercial animal feed samples, aflatoxin M1 (AFM1) raw dairy milk (n = 20) collected from Assiut City Upper Egypt. Multi-mycotoxin immunoaffinity columns were used for cleanup mycotoxin purification. An HPLC–FLD system with an on-line post-column photochemical derivatization detection target toxins. AFB1...
Deoxynivalenol (DON) and octyl gallate (OG) are prevalent compounds in the environment food. DON is frequently detected cereals such as corn wheat, while OG commonly employed a food additive. As result, human exposure to these substances inevitable. Given this, objective of this experiment was investigate impact co-exposure (10 μg/kg) on intestinal inflammation. The RAW264.7 macrophage cell line utilized analyze cytokine levels well proteomic metabolomic changes. In quantitative real-time...
There has been a significant concern regarding the potential health risks for humans and animals via foods feeds that are contaminated with different agents. Particularly, mycotoxin contamination is of great importance as it widespread unpreventable. In both feeds, molds produce secondary metabolites called mycotoxins; these produced generally after fungi reach their maturity. Depending on definition used, hundreds fungal compounds recognized mycotoxins. However, attention mainly focused...
This study was conducted to investigate the natural co-occurrence of multiple toxic fungal and bacterial metabolites in sugarcane grass juice intended for human consumption Upper Egypt. Quantification target analytes has been done using "dilute shoot" approach followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total number 29 33 different were detected 21 40 samples, respectively, with a trend concentrations being higher than juice. Among regulated mycotoxins, only...
The natural co-occurrence of 42 mycotoxins was investigated in unprocessed oat grains grown Ireland. sample set included a total 208 crops harvested during 2015–2016 and produced using conventional, organic, or gluten free farming systems. A range different toxins identified, including the major type (neosolaniol, HT-2 T-2 toxins, triol, T-2-glucoside, co-occurring 21 samples) B trichothecenes (deoxynivalenol, nivalenol, deoxynivalenol-3-glucoside), enniatins (B1, B, A1, 12 samples), as well...
Aflatoxin B1 (AFB1) and fumonisin (FB1) are widely (co-)detected in food known for their hepatotoxicity humans. Still, combined toxicity needs to be investigated, especially the impact on mitochondria. In our previous work, we examined effect of short-term exposure different doses AFB1, FB1, binary mixture (MIX) bioenergetic status HepG2 cells, a well-recognized vitro model system studying liver cell function. current further investigated (combined) AFB1 FB1 mitochondrial glycolytic activity...
Abstract Plant diseases are often caused by a consortium of pathogens competing with one another to gain foothold in the infection niche. Nevertheless, studies limited single pathogen on its host. In Europe, fusarium head blight (FHB) wheat is multiple Fusarium species, including graminearum and F. poae . Here, we combined time series (co)inoculations, monitored multispectral imaging, transcriptional, mycotoxin analyses, study temporal interaction between both species wheat. Our results...
Fumonisin B1 (FB1) and aflatoxin (AFB1) are frequent contaminants of staple foods such as maize. Oral exposure to these toxins poses health hazards by disrupting cellular signaling. However, little is known regarding the multifaced mitochondrial dysfunction-linked toxicity FB1 AFB1. Here, we show that after AFB1, respiration significantly decreased measuring oxygen consumption rate (OCR), membrane potential (MMP) reactive species (ROS). The current work shows integrity mitochondria (MMP...
The LC-MS-based method has emerged as the preferred approach for quantifying food allergens. However, preparation of a traditional calibration curve (MSCC) is labor-intensive and error-prone. Here, sensitive robust LC-MS/MS 10 major allergens was developed validated, where one-sample multipoint external (OSCC) employed instead MSCC. By employing multiple isotopologue reaction monitoring (MIRM) technique with only one spiked level in blank, OSCC can be effectively established. Results...
Objectives To compare the mid‐term safety and efficacy of holmium laser enucleation prostate versus bipolar transurethral resection in management large‐volume benign prostatic hyperplasia. Methods From December 2016 to March 2018, patients with hyperplasia (≥80 cc) were randomized (block randomization, computer‐generated random list) (57 patients) or (55 patients). Patients excluded if they had an International Prostate Symptom Score <13, a maximum urinary flow rate >15 mL/s presence...