A5023056759- Animal Nutrition and Physiology
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Meat and Animal Product Quality
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Metabolomics and Mass Spectrometry Studies
- Peroxisome Proliferator-Activated Receptors
- Adipokines, Inflammation, and Metabolic Diseases
- Genetic and phenotypic traits in livestock
- RNA modifications and cancer
- Lipid metabolism and biosynthesis
- Animal Genetics and Reproduction
- Biochemical Analysis and Sensing Techniques
- Marine Biology and Ecology Research
- Plant Reproductive Biology
- Livestock and Poultry Management
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Reproductive Biology and Fertility
- Genetic Mapping and Diversity in Plants and Animals
- Hypothalamic control of reproductive hormones
- FOXO transcription factor regulation
- Isotope Analysis in Ecology
Sichuan Agricultural University
2021-2025
Ministry of Agriculture and Rural Affairs
2023-2025
Ningbo University
2023
Knowledge of the breed-specific and tissue-specific lipid molecular composition is helpful to understanding complicated mechanisms deposition. In this study, a liquid chromatography (LC)-mass spectrometry (MS)-based lipidomics approach was used discriminate chicken meat abdominal fat from different sources. OxPC (16:0-20:4+1O(1Cyc)) PC (18:2/20:5) were determined as discriminatory markers for Guangyuan grey chicken, Jiuyuan black Tibetan chicken. A total 209 molecules commonly marker...
The composition and content of amino acids in foodstuffs have a vital impact on the nutritional value taste. With aim understanding nutrition flavor Guangyuan grey chicken, pectoralis thigh muscle chickens at age 90 d, 120 d 150 were determine using liquid chromatography–tandem mass spectrometry (LC-MS/MS) an acid analyzer. A total 17 detected both via analyzer, which glutamate was highest. Additionally, 21 deproteinized free LC-MS/MS. Among all samples, glutamine histidine In terms (FAAs),...
Intramuscular fat (IMF) is one of the crucial factors determining meat quality. IMF deposition depends on hyperplasia and hypertrophy intramuscular preadipocytes, in which genes noncoding RNAs play an important regulatory role. According to previous transcriptome analysis, ANXA6 miR-24-3p were identified as involved lipid metabolism breast muscle. In this study, we further investigated their function proliferation differentiation chicken preadipocytes. The results indicated that...
Steroid hormones secreted by granulosa cells are essential for maintaining normal development of chicken follicles. Our previous sequencing data indicated that miR-181b-5p and RAS-related protein 1B (RAP1B) appeared to function in cells, which was further explored this study. The results suggested facilitated the aggregation lipid droplets synthesis progesterone. In contrast, RAP1B astricted deposition progesterone secretion. Cotransfection overexpression vector with mimic eliminated...
To deeply understanding the impact of peripheral energy level on development ovaries during sexual maturation chicken, in this study, and serum sexually mature immature chickens at same age from different groups were collected, proteome metabolome detected. The results ovarian metabolomics revealed that dietary levels affected metabolism fatty acid oxidation ovary including up-regulated expression dihydroacetone phosphate α-linolenic high groups. proteomics showed catecholamine biosynthesis...
Sexual maturity is a crucial factor in the formation and development of poultry reproductive capacity. The nutritional status has been confirmed to play an important role regulation sexual maturity. To investigate effect dietary energy levels on chicken, diets with three (group L: 2573 kcal/kg, group C: 2836 H:3122 kcal/kg) were implemented feed Guangyuan Grey chickens. During this trial, body weight, size, organ development, maturity, performance blood biochemical parameters monitored....
Broodiness, a maternal behavior, is accompanied by the atresia of follicles and serious degradation poultry reproductive performance. The comparison between brooding laying hens usually an ideal model for exploring regulation mechanism follicle atresia. In this study, we selected three to collect their whole transcriptome sequencing. results demonstrated different expression patterns hens. top 10 differentially expressed genes with highest expression, MMP10 was relatively low in hens, but...
The liver and ovary perform a vital role in egg production hens. In the later laying period, egg-laying capacity of female hens, particularly that local breeds, declines significantly. Hence, it is essential to study features conditions during this period. research, we characterized proteins metabolites 55-week-old Guangyuan gray chickens (Group G) Hy-Line H) by using liquid chromatography chip/electrospray ionization quadruple time-of-flight/mass spectroscopy (LC-MS/MS). total, 139...
For commercial laying hens, the continuous high-intensity ovulation process leads to a significant accumulation of reactive oxygen species (ROS) in granulosa cells, inducing oxidative stress, which accelerates ovarian aging and shortens peak period. The molecular mechanisms underlying this remain poorly understood. Therefore, we modeled processes stress antioxidant chicken cells. Small RNA sequencing revealed that miR-15c-3p expression was elevated by induction attenuated curcumin....
Abstract Probiotics are expected to be an ideal alternative for antibiotics in the poultry industry. This study aimed investigate effect of Lactobacillus plantarum on growth traits, slaughter performance, serum markers and intestinal bacterial community Daheng broilers. A total 2400 healthy one‐day‐old broilers were randomly divided into 5 groups with 6 replicates per group 40 individuals replicate. Birds control fed a basal diet, others diets supplemented 10 , 7 8 CFU/kg respectively. It...
In diploid organisms, interactions between alleles determine phenotypic variation. previous experiments, only MYH1F was found to show both ASE (spatiotemporal allele-specific expression) and TRD (allelic transmission ratio distortion) characteristics in the pectoral muscle by comparing genome-wide allele lists of hybrid populations (F1) meat- egg- type chickens. addition, is a member MYH gene family, which plays an important role skeletal non-muscle cells animals, but specific expression...
Skeletal muscle can undergo a regenerative process in response to injury or disease maintain quality and function. Myogenesis depends on the proliferation differentiation of myoblasts, miRNAs balance between them by precisely regulating many key factors myogenic network. Here, we found that miR-136-5p was significantly upregulated during C2C12 cells. We demonstrate acts as negative regulator development mouse myoblasts. In terms mechanism, inhibits formation β-catenin/LEF/TCF DNA-binding...
Aged chickens are often a secondary dietary choice, owing to the poor organoleptic qualities of their meat. With regard meat quality chickens, metabolic profiles pectoral muscle in Guangyuan grey (group G) and Hy-Line hens H) aged 55 weeks were compared via ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A total 74 metabolites identified with differential changes ion model. Lipids lipid-like molecules comprised largest proportion among different...
The quail is an important research model, and the demand for meat has been increasing in recent years; therefore, it worthwhile investigating development of embryonic skeletal muscle expression patterns regulatory genes. In this study, MyoD Pax7 breast (m. pectoralis major) leg biceps femoris) embryos on days 10 through 17 were determined using qRT-PCR. Paraffin sections analyzed to characterize changes over time. Results showed that expressed both muscles played a significant role...
The aim of this study is to assess the differences in meat quality, nutritional composition, carcass traits, and myofiber characteristics between Hy-Line grey chickens (HLG, commercial breed) Guangyuan (GYG, indigenous breed). A total 20 55-week-old were selected for slaughter. HLG exhibited a larger weight, breast muscle abdominal fat weight (p < 0.05). GYG higher crude protein content, lower shear force, smaller fiber size thigh muscles, whereas presented pH values inosine-5'-monophosphate...