A5015400839- Animal Nutrition and Physiology
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Meat and Animal Product Quality
- Calpain Protease Function and Regulation
- Genetic and phenotypic traits in livestock
- Livestock and Poultry Management
- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Silk-based biomaterials and applications
- interferon and immune responses
- Adipokines, Inflammation, and Metabolic Diseases
- Peroxisome Proliferator-Activated Receptors
- Probiotics and Fermented Foods
- Animal Genetics and Reproduction
- Inflammatory Bowel Disease
- Genetic Mapping and Diversity in Plants and Animals
- Biochemical Analysis and Sensing Techniques
- Lipid metabolism and biosynthesis
- Genital Health and Disease
- Wind and Air Flow Studies
- Neurogenetic and Muscular Disorders Research
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Sichuan Animal Science Academy
2010-2024
Lanzhou University
2023
Lanzhou University Second Hospital
2023
Guangdong Provincial Hospital of Traditional Chinese Medicine
2021
Soochow University
2021
First Affiliated Hospital of Soochow University
2021
Poultry Research Institute
2020
Guangdong Province Women and Children Hospital
2017-2018
Research Institute of Animal Husbandry
2008
Wuhan Engineering Science & Technology Institute
2000
Abstract Tibetan Chickens should have unique gastrointestinal microbiota because of their particular habitats. Thus, the aim this study was to investigate cecal from five typical high‐altitude regions China. Lohmann egg‐laying hens ( LM s) and Daheng broiler chickens DH were chosen as controls. The bacterial populations surveyed by high‐throughput sequencing HTS ) 16S rRNA hypervariable region V3‐V4 (16S rRNAV 3‐V4) combined with community‐fingerprinting analysis gene based on polymerase...
There are hundreds of phenotypically distinguishable domestic chicken breeds or lines with highly specialized traits worldwide, which provide a unique opportunity to illustrate how selection shapes patterns genetic variation. many local in China.Here, we population genome landscape variations 86 chickens representing 10 diverse breeds. Genome-wide analysis indicated that sex chromosomes have less diversity and under stronger than autosomes during domestication adaptation. We found an...
Due to the increasing demand for producing chickens with high meat quality, there is a need determine its mode of action on chicken quality traits across wider age spectrum. In this study, five groups 200 male Da-Heng type birds were reared until slaughter 60, 90, 120, 150, 180 days old and breast muscle performance, traits, myofiber characteristics evaluated. The larger body weight are based diameter area, less density older than younger birds. There was an effect all (p < 0.05). Older...
The proliferation and differentiation of skeletal muscle satellite cells (SMSCs) play an important role in the development muscle. Our previous sequencing data showed that miR-21-5p is one most abundant miRNAs chicken Therefore, this study, spatiotemporal expression its effects on chickens were explored using vitro cultured SMSCs as a model. results study was highly expressed chickens. overexpression promoted evidenced by increased cell viability, number proliferative phase, mRNA protein...
The egg production performance of chickens is affected by many factors, including genetics, nutrition and environmental conditions. These factors all play a role in affecting the development follicles. MicroRNAs (miRNAs) are important non-coding RNAs that regulate biological processes targeting genes or other after transcription. In animal reproduction process, miRNA known to affect atresia follicles regulating apoptosis autophagy granulosa cells (GCs).In this study, we identified potential...
Objective: The goal of this study was to investigate the mechanisms muscle growth and development three chicken breeds. Participants: Eighteen chickens, including different breeds with speeds (White Broiler, Daheng, Commercial Layers Roman), were used. Methods: Total RNA from breast these chickens subjected a gene expression microarray. Differentially expressed genes (DEGs) screened functional enrichment analysis performed using DAVID. Seven DEGs confirmed by quantitative reverse...
Follicular atresia is an important cause of reproductive decline in egg-laying hens. Therefore, a better understanding the regulation mechanism follicle poultry measure to maintain persistent high egg performance. However, how role regulatory relationship between autophagy and apoptosis intrafollicular environment affects follicular chickens remain unclear. The objective this study was explore molecular mechanisms regard atresia. 20 white leghorn layers (32-wk-old) were equally divided into...
Abstract Background The aim of this study is to screen single nucleotide polymorphisms (SNP) chicken Calpain3 ( CAPN3 ) gene and analyze the potential association between carcass traits in chickens. We screened 307 meat-type quality from 5 commercial pure lines (S01, S02, S03, S05, D99) 4 native breeds Guangdong Province (Huiyang Huxu Qingyuan Ma chicken) Sichuan (Caoke Shandi Black-bone chicken), China. Results Two SNPs (11818T>A 12814T>G) were detected by strand conformation...
This study was aimed at investigating the effect of calpain 1 (CAPN1) gene on carcass and meat quality traits in eight meat-type chicken populations, including five pure lines (developed from Chinese local breeds) three cross-breeds. Primer pairs for Coding Sequence (CDS) region CAPN1 were designed genomic sequence database. Polymorphisms detected by polymerase chain reaction (PCR)-Single Strand Conformation Polymorphism (SSCP) DNA sequencing. Three single nucleotide polymorphisms (SNP;...
Growth hormone (GH) plays a diverse role in animals together with other hormones of somatotropic axis. In the current research, chicken GH (cGH) as candidate gene affecting carcass traits was investigated chickens from 2 local breeds [Mountainous Black-Bone (Wugu) and Caoke chicken] Sichuan province, 1 pure line quality (Sanhuang chicken) Guangdong commercial crossbreds. The RFLP method used to identify polymorphisms cGH gene. Three restriction enzyme polymorphic sites were detected Sequence...
Background Tibetan chickens living at high altitudes show specific adaptations to high-altitude conditions, but the epigenetic modifications associated with these have not been characterized. Results We investigated genome-wide DNA methylation patterns in chicken blood by using whole genome bisulfite sequencing. Generally, exhibited analogous that of lowland chickens. A total 3.92% genomic cytosines were methylcytosines and 51.22% CG contexts methylated, which was less than those (55.69%)....
The oviduct plays an indispensable role in the formation of eggs, especially magnum and uterus. identification development different stages will help to target candidate genes pathways regulation albumen eggshell formation, as well defense mechanism egg.To identify function differences molecular egg, we performed transcriptome sequencing analysis uterus 120-d-old 300-d-old Lohmann layers, three birds each group.With fold changes (log2 ratio) ≥ 2 false discovery rate (FDR) < 0.01, RNA-Seq...
Broodiness, a maternal behavior, is accompanied by the atresia of follicles and serious degradation poultry reproductive performance. The comparison between brooding laying hens usually an ideal model for exploring regulation mechanism follicle atresia. In this study, we selected three to collect their whole transcriptome sequencing. results demonstrated different expression patterns hens. top 10 differentially expressed genes with highest expression, MMP10 was relatively low in hens, but...
Dear Editor, Zinner's syndrome is an uncommon urogenital malformation of congenital seminal vesicle cyst associated with ipsilateral renal agenesis. It considered to be the anomaly development in early embryogenesis affecting distal part Mullerian duct.1 After initial report by Zinner 1914,2 fewer than 100 cases have been reported literature.3 On basis a children research Taipei, incidence agenesis was 0.0046% (13/280 000).1 For symptomatic patients, integrated and accurate diagnosis from...
Recent studies in mice suggested that KLF5 (Kruppel like factor 5), a zinc-finger transcription factor, plays an important role skeletal muscle development and regeneration. As the process of development, participates regulation cell cycle, survival, dryness under different environmental conditions, but it is not clear whether atrophy. Therefore, we investigated can regulate atrophy chicken satellite cells vitro examined its mechanism action. qPCR showed gene knockdown promoted expression...
As a ubiquitous heavy metal, cadmium (Cd) is highly toxic to various organs. However, the effects and molecular mechanism of Cd toxicity in chicken heart remain largely unknown. The goal our study was investigate cardiac injury chickens' exposure Cd. We detected levels oxidative stress–related molecules Cd-induced heart, assessed histopathological changes by hematoxylin eosin staining. RNA sequencing performed identify differentially expressed mRNAs between group control group. expression...
Skeletal muscle, comprising approximately 40% of body mass, is a highly complex and heterogeneous tissue serving multitude functions in the organism. Non-coding RNAs (ncRNAs) are known to participate skeletal muscle development as critical regulators. However, regulatory mechanisms ncRNAs on chicken traits not well understood. In present study, we collected leg from male embryos Tibetan at embryonic (E) 10 E18 for RNA sequencing. A total 6,583 differentially expressed mRNAs (DEMs) including...