A5087272338- Angiogenesis and VEGF in Cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Neurological Disease Mechanisms and Treatments
- Nerve injury and regeneration
- Nuclear Receptors and Signaling
- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- MRI in cancer diagnosis
- Acute Ischemic Stroke Management
- Bioactive Compounds and Antitumor Agents
- Medicinal Plant Studies
- Neuroscience and Neuropharmacology Research
- S100 Proteins and Annexins
- Inflammatory mediators and NSAID effects
- Cerebrovascular and Carotid Artery Diseases
- Cancer, Hypoxia, and Metabolism
- Metabolism and Genetic Disorders
- RNA regulation and disease
- Nitric Oxide and Endothelin Effects
- Protein Tyrosine Phosphatases
- Galectins and Cancer Biology
- Eicosanoids and Hypertension Pharmacology
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
Institute of Biomedical Sciences, Academia Sinica
2012-2023
National Defense Medical Center
2002-2016
National Yang Ming Chiao Tung University
2013
Academia Sinica
1998-2013
National Yang Ming University Hospital
2013
National Health Research Institutes
2009
The University of Texas Health Science Center at Houston
2005-2009
National Cheng Kung University
2009
University of North Carolina at Chapel Hill
2009
Ann Arbor VA Medical Center
2009
Infarct volume is one of the common indexes for assessing extent ischemic brain injury following focal cerebral ischemia. Accuracy in measurement infarct compounded by postischemic edema that may increase infarcted region. We evaluated effect on determined triphenyltetrazolium chloride and hematoxylin eosin stains a ischemia model rats. In middle artery occlusion rats, infarction confined to cortex. The was delineated stain and, selected samples, stain. size at different times after insult...
Abstract The expression of the protooncogenes, c‐ fos , jun B, and D was investigated in a rat focal cerebral ischemia model by Northern analysis situ hybridization. Severe (reduction regional blood flow 88‐92%) this is confined to cortex irrigated right middle artery. Ischemia for 30 minutes, which caused only slight cortical damage (infarct size, <10 mm 3 ), induced both B mRNAs exclusively cortex. 90 led large infarction >140 also these two genes as well ipsilateral hippocampus....
Growth-associated protein 43 (GAP43), a kinase C (PKC)-activated phosphoprotein, is often implicated in axonal plasticity and regeneration. In this study, we found that GAP43 can be induced by the endotoxin lipopolysaccharide (LPS) rat brain astrocytes both vivo vitro. The LPS-induced astrocytic expression was mediated Toll-like receptor 4 nuclear factor-κB (NF-κB)- interleukin-6/signal transducer activator of transcription 3 (STAT3)-dependent transcriptional activation. overexpression PKC...
Background and Purpose— Angiogenesis occurs after cerebral ischemia, the extent of angiogenesis has been correlated with survival in stroke patients. However, postischemic is short-lived may be completely terminated within a few weeks ischemic insult. The molecular mechanism underlying dissolution angiogenic processes poorly understood. Although expression genes studied models, activation angiostatic ischemia not investigated. Thrombospondin (TSP)-1 TSP-2 are naturally occurring factors,...
The angiopoietin/Tie receptor system may contribute to angiogenesis and vascular remodeling by mediating interactions of endothelial cells with smooth muscle pericytes. temporal expression angiopoietin-1 (Angpo-1), angiopoietin-2 (Angpo-2), Tie-1, Tie-2 mRNA was studied in a focal cerebral ischemia model rats. cDNA fragments obtained from reverse transcription polymerase chain reaction amplification were cloned used as probe detect individual genes. Northern blot analysis showed delayed...
Brain expresses abundant lipocalin-type prostaglandin (PG) D2 (PGD2) synthase but the role of PGD2 and its metabolite, 15-deoxy-Delta(12,14) PGJ2 (15d-PGJ2) in brain protection is unclear. The aim this study to assess effect 15d-PGJ2 on neuroprotection.Adenoviral transfer cyclooxygenase-1 (Adv-COX-1) was used amplify production ischemic cortex a rat focal infarction model. Cortical Adv-COX-1-treated rats increased by 3-fold over control, which correlated with reduced infarct volume activated...
Postischemic cerebral blood flow and volume changes have been associated with angiogenesis; nevertheless, the spatiotemporal in vascular permeability, density, vessel size not investigated. Here we report a prolonged increase permeability from day 3 to 21 after ischemia, particular reperfused outer cortical layers leptomeninges. Increased (CBV) was observed 14, whereas increased small vessels, primarily capillaries, noticed 7 14 cortex. An initial decrease density reciprocal were within...
Thiazolidinediones have been reported to protect against ischemia-reperfusion injury. Their protective actions are considered be peroxisome proliferator-activated receptor-gamma (PPAR-gamma)-dependent; however, it is unclear how PPAR-gamma activation confers resistance injury.We evaluated the effects of rosiglitazone or overexpression on cerebral infarction in a rat model and investigated antiapoptotic N2-A neuroblastoma cell model. Rosiglitazone significantly reduced infarct volume. The...
Abstract To determine the involvement of peroxisome proliferator‐activated receptor‐γ (PPAR‐γ) in cytoprotection, we subjected N2‐A cells to oxygen–glucose deprivation followed by reoxygenation (H‐R). Following H‐R insults, H 2 O production was increased while cell viability declined, which accompanied loss mitochondrial membrane potential (MMP), cytochrome c release, caspases 9 and 3 activation, poly(ADP‐ribose)polymerase (PARP) cleavage apoptosis. Rosiglitazone up 5 µM protected viability,...
Psoriasis is a chronic inflammatory skin disease characterized by cell infiltration, as well hyperproliferation of keratinocytes in lesions, and considered metabolic syndrome. We found that the expression galectin-7 reduced lesions patients with psoriasis. IL-17A TNF-α, 2 cytokines intimately involved development psoriatic suppressed human primary (HEKn cells) immortalized keratinocyte line HaCaT. A knockdown these cells elevated production IL-6 IL-8 enhanced ERK signaling when were...
Background and Purpose— Angiogenesis occurs after cerebral ischemia, but the relationship between angiogenesis hemodynamic change is unknown. The aim of present study was to investigate ischemia-induced hemodynamics in a well-defined 3-vessel occlusion model rat by using diffusion- (DWI), perfusion-, T2-weighted MRI (T2WI). Methods— Rats were subjected 60 minutes transient middle artery or sham operation. DWI T2WI used characterize extent ischemic lesion from 4.5 hours 14 days reperfusion. A...
Abstract Although mRNA expression of group IIA secretory phospholipase A 2 (sPLA ‐IIA) has been implicated in responses to injury the CNS, information on protein remains unclear. In this study, we investigated temporal and spatial sPLA ‐IIA immunoreactivity transient focal cerebral ischemia induced rats by occlusion middle artery. Northern blot analysis showed a biphasic increase following 60‐min ischemia–reperfusion: an early phase at 30 min second late ranging from 12 h 14 days. situ...
Young-onset hypertension has a stronger genetic component than late-onset counterpart; thus, the identification of genes related to its susceptibility is critical issue for prevention and management this disease. We carried out two-stage association scan map young-onset genes. The first-stage analysis, genome-wide study, analyzed 175 matched case-control pairs; second-stage confirmatory verified results at first stage based on total 1,008 patients controls. Single-locus tests, multilocus...
Background — We tested the hypothesis that bicistronic cyclooxygenase-1 (COX-1)/prostacyclin synthase (PGIS) and COX-1 gene transfer reduce cerebral infarct volume by augmenting synthesis of protective prostaglandins. Methods Results infused into lateral ventricle a rat stroke model recombinant adenoviruses (rAd) containing (Adv-COX-1), PGIS (Adv-COX-1/PGIS), or Adv-PGK control vector, we determined protein eicosanoid levels volume. proteins were increased in time-dependent manner....
Angiogenesis is induced in response to ischemia. Thrombospondin-1 (TSP-1) a potent angiostatic factor. Silencing of TSP-1 expression may contribute the postischemic angiogenesis. Upregulation TSP-1, contrast, terminate A possible mechanism that silences DNA methylation its promoter region. has been reported following cerebral The present study aimed explore whether region regulates after oxygen—glucose deprivation (OGD) murine endothelial cells (CECs) vitro. Sublethal OGD increased extent...
Early human embryonic stem cell (hESC)-derived neural populations consist of various progenitors (ENPs) with broad developmental propensity. Here, we sought to directly convert somatic cells into ENP-like phenotypes using hESC-ENP-enriched transcription factors (TFs). We demonstrated that induced ENP could be efficiently converted from fibroblasts two TF combinations. The iENPs exhibit cellular and molecular characteristics resembling hESC-ENPs can give rise astrocytes, oligodendrocytes,...
Tie-1 and Tie-2 are receptor tyrosine kinases (RTKs) that exclusively expressed in endothelial cells play important roles cell biology. The authors have reported previously the temporal profiles of mRNA expression after focal cerebral ischemia-reperfusion. In current study, localization Tie-1/Tie-2 proteins were further investigated same ischemia model. situ hybridization showed that, 60-minute 72-hour reperfusion, both appeared as capillary-like structures ischemic middle artery (MCA)...