A5082871442- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Immune Response and Inflammation
- Kruppel-like factors research
- Pluripotent Stem Cells Research
- Cytomegalovirus and herpesvirus research
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Cancer Research and Treatments
- Lymphoma Diagnosis and Treatment
- IL-33, ST2, and ILC Pathways
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Hematopoietic Stem Cell Transplantation
- Erythrocyte Function and Pathophysiology
- Whipple's Disease and Interleukins
- Exercise and Physiological Responses
- Pancreatic function and diabetes
- interferon and immune responses
- Cancer, Stress, Anesthesia, and Immune Response
Institute of Molecular Biology, Academia Sinica
2015-2025
National Defense Medical Center
2004-2025
Academia Sinica
2000-2025
Genomics Research Center, Academia Sinica
2024
National Defense Medical College
2009
Taipei Medical University
2009
University of California, Berkeley
1991-1992
Massachusetts Institute of Technology
1989-1991
Center for Cancer Research
1988-1991
Illinois State University
1987-1989
The role of major histocompatibility complex (MHC) class I expression in natural killer (NK) cell target recognition is controversial. Normal T blasts from MHC I-deficient mutant mice were found to serve as cells for NK vitro, which suggests that molecules are directly involved recognition. Spleen the deficient their ability lyse or NK-susceptible tumor targets, and could not reject allogeneic bone marrow. Thus, may participate positive selection tolerance induction cells.
Metabolic pathways and inflammatory processes are under circadian regulation. Rhythmic immune cell recruitment is known to impact infection outcomes, but whether the clock modulates immunometabolism remains unclear. We find that molecular Bmal1 induced by stimulants, including Ifn-γ/lipopolysaccharide (M1) tumor-conditioned medium, maintain mitochondrial metabolism metabolically stressed conditions in mouse macrophages. Upon M1 stimulation, myeloid-specific knockout (M-BKO) renders...
Natural killer (NK) cells can control metastasis through cytotoxicity and IFN-γ production independently of T in experimental mouse models. The inverse correlation between NK activity incidence supports a critical role for human metastatic surveillance. However, autologous cell therapy has shown limited benefit treating patients with solid tumors. Using spontaneous model MHC-I + breast cancer, we found that transfer IL-15/IL-12-conditioned syngeneic after primary tumor resection promoted...
The specificity of T cells bearing gamma delta T-cell receptors (gamma delta+ cells) is poorly characterized. Earlier studies suggest that like alpha beta+CD8+ cells, some may recognize antigens associated with class I major histocompatibility complex molecules. are nearly absent in I-deficient mice (mutant for beta 2-microglobulin), reflecting a requirement intrathymic "positive selection" these by Here, we examine whether the development altered 2-microglobulin mutant mice. We show...
We report here a mAb, 14-2, reactive with TCRs that include V beta 14. The frequency of 14+ T cells varies CD4 and CD8 subset is controlled by the H-2 genes. Thus CD8+ from H-2b mice approximately 2.3% while expressing K kappa greater than 8% cells. In all strains examined, 7-8% CD4+ express frequent usage 14 in kappa-expressing result preferential positive selection as demonstrated analysis radiation chimeras. These studies demonstrate H-2-dependent occurs unmanipulated mice. Furthermore,...
Hypoxic-ischemia (HI) and inflammation are the two major pathogenic mechanisms of brain injury in very preterm infants. The neurovascular unit is target HI immature brain. Systemic may worsen by up-regulating neuroinflammation disrupting blood–brain barrier (BBB). Since neurons oligodendrocytes, microvascular endothelial cells, microglia closely interact with each other, there be a common signaling pathway leading to damage after TNF-α key pro-inflammatory cytokine that acts through TNF...
Abstract Mice that lack IL-15 or the IL-15R α-chain (IL-15Rα) are deficient in peripheral CD8+, but not CD4+, T cells. This CD8+ cell-specific deficiency has now been investigated further by characterization of a new strain IL-15Rα−/− mice. The adult mutant mice exhibited specific reduction percentage CD8-single positive TCRhigh thymocytes. expression Bcl-2 was reduced both thymocytes and naive cells animals, susceptibility these to death increased. Memory were profoundly IL-15Rα−/−mice,...
Cyclophosphamide (CTX) treatment in vivo kills proliferating tumor cells by DNA crosslinking, however, suppression of growth CTX several murine models requires CD8+ T cells. Given that induces damage and type I interferon (IFN-I), we investigated the role host cGAS STING anti-tumor effect vivo. Using a metastasized EO771 breast cancer model with chromosomal instability, found therapy long-term survival mice this outcome being dependent on cGAS/STING bone marrow (BM)-derived Furthermore, 1...
Cyclophosphamide (CTX) treatment in vivo kills proliferating tumor cells by DNA crosslinking; however, the suppression of growth CTX several murine models requires CD8+ T cells. Given that induces damage and type I interferon (IFN-I), we investigated role host cGAS STING anti-tumor effect vivo. A metastasized EO771 breast cancer model with chromosomal instability bone marrow (BM) chimera approach were used this study. We found therapy long-term survival mice, outcome being dependent on...
NK cell development requires IL-15, which is "trans-presented" to IL-15Rβγ on cells by IL-15Rα other cells. In this study, we report that different levels of IL-15 trans-presentation are required for developmental events reach full maturation status. Because the intracellular domain has capacity recruit signaling molecules, generated knockin and transgenic (Tg) mice lack assess role level independent function domain. The various these follows order WT > Tg6 Tg1 ≥ knockout. Bone marrow...
Abstract The nucleotide‐binding and oligomerization domain, leucine‐rich repeats, pyrin domain‐containing protein 3 (NLRP3) inflammasome plays a crucial role in innate immunity is involved the pathogenesis of autoinflammatory diseases. Glycolysis regulates NLRP3 activation macrophages. However, how lactic acid fermentation pyruvate oxidation controlled by mitochondrial carrier (MPC) affect disease remains elusive. We found that inactivation MPC with genetic depletion or pharmacological...
Abstract Mice devoid of the IL-15 system lose over 90% CD8αα+ TCRαβ and TCRγδ intestinal intraepithelial lymphocytes (iIELs). Previous work revealed that IL-15Rα expressed by parenchymal cells, but not bone marrow-derived are required for normal iIEL homeostasis. However, it remains unclear when how affects iIELs through their development. This study found is dispensable thymic stage development maintenance and/or differentiation putative lineage marker negative precursors in epithelium,...
Abstract NK cell development and homeostasis require IL-15 produced by both hematopoietic parenchymal cells. Certain sources, such as macrophages dendritic cells, are known, whereas the source of remains elusive. Using two types adipocyte-specific Il15−/− mice, we identified adipocytes a that supported nonredundantly. Both mice showed reduced production specifically in adipose tissue but impaired spleen liver addition to tissue. We also found harbored progenitors other niches (e.g. spleen)...
IL ‐15 is an essential survival factor for CD 8αα + intestinal intraepithelial lymphocytes (i IEL s) in vitro and vivo. However, the ‐15‐induced signals primary i s remains elusive. Although B cl‐2 level positively correlates with R α expression epithelial cells, overexpression of only moderately restores γδ Il15 −/− mice. Here, we found that promptly activated a J ak3‐ ak1‐ PI 3 K ‐ A kt pathway led to upregulation M cl‐1. This also induced delayed but sustained ERK 1/2 activation, which...
We have examined the usage of V beta 5.1 region in peripheral T cell populations several mouse strains by measuring transcript levels. The results show that is expressed predominantly CD8+ cells mice MHC haplotypes. This finding suggests may confer restriction to class 1 molecules present these strains. Interestingly, however, expressing 5.1, like those 17a and 11, are clonally deleted from both CD4 CD8 subsets express IE molecules. latter result implies confers reactivity a 2 molecule (IE)....
Interleukin 15 (IL-15) is thought to be abundant in the skeletal muscle under steady state conditions based on RNA expression; however, IL-15 level may not reflect protein due post-transcriptional regulation. Although exogenous treatment and overexpression studies indicated functions muscle, how cell uses remains unclear. In myositis patients, up-regulated muscle. Given supporting role of CD8(+) T-cell survival activation pathogenic cytotoxic T cells polymyositis inclusion-body myositis, we...