A5086058190- Reproductive System and Pregnancy
- Bee Products Chemical Analysis
- Immunotherapy and Immune Responses
- Pregnancy and preeclampsia studies
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Essential Oils and Antimicrobial Activity
- Extracellular vesicles in disease
- Antifungal resistance and susceptibility
- MicroRNA in disease regulation
- Fungal Infections and Studies
- Nail Diseases and Treatments
- T-cell and B-cell Immunology
- Immune cells in cancer
- Circadian rhythm and melatonin
- Virus-based gene therapy research
- Birth, Development, and Health
- vaccines and immunoinformatics approaches
- Fungal Biology and Applications
- Autophagy in Disease and Therapy
- Nanoparticle-Based Drug Delivery
- Hepatitis C virus research
- T-cell and Retrovirus Studies
- Liver Disease Diagnosis and Treatment
- Medical and Biological Ozone Research
Universidade Estadual Paulista (Unesp)
2009-2023
Universidade do Oeste Paulista
2020-2023
Universidade de São Paulo
2013-2022
Universidade Estadual de Londrina
2005
Istituto Dermopatico dell'Immacolata
2003
University of Padua
1986
Preeclampsia (PE) is a human pregnancy-specific syndrome with abnormal activation of cells from the innate immune system. The present study evaluated whether silibinin (SB) treatment monocytes preeclamptic women could modulate NLRP1 and NLRP3 inflammasomes as well TLR4/NF-κB pathway activation. Peripheral blood 20 normotensive (NT) pregnant women, THP-1 cell line, were cultured or without monosodium urate (MSU) SB. NLRP1, NLRP3, Caspase-1, TLR4, MyD88, NF-κB, IL-1β, IL-18, TNF-α IL-10 gene...
Pre-eclampsia (PE) is an obstetric pathology characterized by abnormal activation of the innate and adaptive immune systems dependent on imbalance T helper subsets. The present study aimed to evaluate gene protein expression type 1 (Th1)/Th2/Th17/regulatory (Treg) cell transcription factors in peripheral blood lymphocytes from pregnant women with PE employing quantitative RT-PCR flow cytometry techniques, as well cytokine profile produced these CD4+ T-cell subsets plasma PE, classified...
: The aim of the current study is to present a strategy improve efficiency 5-fluorouracil (5-FU), which widely used as antineoplastic agent against solid tumors-based on use gold nanocarriers overcome resistance colorectal cancer cells. 5-FU was loaded nanoparticles (AuNP) coated with anti-EGFR antibodies in order target them towards cells that overexpress epidermal growth factor receptors (EGFR). Physicochemical characterization has shown AuNP size approximately 20 nm and functionalization...
The relationships between arbuscular mycorrhizal fungi and root morphological characteristics were studied under greenhouse conditions of 78 tropical native woody species 47 seedling collected in the field. Seedlings pioneer early secondary that generally exhibited fine roots with a dense cover long hairs showed higher response colonization than late-secondary climax coarse sparse short hairs. Root-hair length incidence decreased progression among successional groups while fine-root diameter...
HIV infection and the prolonged use of antiretroviral therapy (ART) contribute to persistent inflammation immune deregulation in people living with HIV/AIDS (PLWHA). Propolis is a bee product plenty biological properties, including immunomodulatory anti-inflammatory action. This work aimed evaluate possible changes immune/inflammatory response PLWHA under ART after propolis intake. Asymptomatic were double-blindly randomized into parallel groups receiving (500 mg/day, n = 20) for 3 months or...
Ovarian cancer (OC) is the most lethal gynecological malignancies and many women develop chemoresistance associated with inflammatory process. We investigated effects of P-MAPA IL-12 on immune responses in a chemically-induced OC model.OCs were induced 7,12-dimethylbenz(a)anthracene into ovarian bursa, animals given (5 mg/kg bw., i.p., twice week), or (300 ng/kg one week) for 60 days, both IL-12. Immunohistochemistry, western blot, flow cytometry, multiplex assay used to examine...
Docetaxel (DTX) is used against breast cancer despite its side effects such as toxicity and immunosuppression. Here we investigated the cytotoxic immunomodulatory of ethanol solution extract propolis (EEP) in combination with DTX on MCF-7 cells women's monocyte. The potential EEP + was assessed by MTT assay type tumor cell death evaluated flow cytometry. migration invasion were analyzed. Cytokine production monocytes ELISA expression surface markers We also fungicidal activity Candida...
Background: Propolis exhibits huge potential in the pharmaceutical industry. In present study, its effects were investigated on dendritic cells (DCs) stimulated with a tumor antigen (MAGE-1) and retinoic acid (RA) T lymphocytes to observe possible differential activation of lymphocytes, driving preferentially Th1 or Treg cells. Methods: Cell viability, lymphocyte proliferation, gene expression (T-bet FoxP3), cytokine production by DCs (TNF-α, IL-10, IL-6 IL-1β) (IFN-γ TGF-β) analyzed....
Immunotherapies have emerged as promising complementary treatments for ovarian cancer (OC), but its effective and direct role on OC cells is unclear. This study examined the combinatory effects of protein aggregate magnesium–ammonium phospholinoleate–palmitoleate anhydride, known P-MAPA, human recombinant interleukin-12 (hrIL-12) cell migration/invasion, apoptosis, toll-like receptor (TLR)-mediated inflammation, cytokine/chemokine profile in line SKOV-3. P-MAPA IL-12 showed toxicity under...
A polysaccharide-rich fraction (ATF) of medicinal mushroom Agaricus brasiliensis was evaluated on the candidacidal activity, H2O2 and nitric oxide (NO) production, expression mannose receptors by murine peritoneal macrophages. Mice received three intraperitoneal (i.p.) injections ATF after 48 h their resident macrophages were assayed against Candida albicans yeast forms. The treatment increased fungicidal activity it associated with higher levels H2O2, whereas NO production not affected. We...
Fungal recognition by Dectin-1 receptor triggers a series of cellular mechanisms involved in protective activation the immune system. In this study, we aimed to evaluate participation induction IL-8, TNF-α, IL-12, IL-10 and IL-17A secretion human monocytes activated with different cytokines, challenged vitro Paracoccidioides brasiliensis (P. brasiliensis). Our results show that P. (Pb265) are able produce IL-17, TNF-α. blockage decreased TNF-α levels indicating such these cytokines. Only...
Paracoccidioidomycosis (PCM), a systemic mycosis endemic in Latin America, occurs after inhalation of mycelial components Paracoccidioides spp. When the fungus reaches lungs and interacts with alveolar macrophages other cells, phagocytic cells such as neutrophils monocytes are immediately recruited to injured site. The interaction between surface molecules pathogens homologous receptors, present on membrane phagocytes, modulates innate immune cell activation. Studies have shown importance...