A5051078345- Hematopoietic Stem Cell Transplantation
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Hepatitis B Virus Studies
- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Lymphoma Diagnosis and Treatment
- Cell Image Analysis Techniques
- Monoclonal and Polyclonal Antibodies Research
- Gene Regulatory Network Analysis
- Virus-based gene therapy research
- Chronic Myeloid Leukemia Treatments
- Immunotherapy and Immune Responses
- Integrated Circuits and Semiconductor Failure Analysis
- COVID-19 and healthcare impacts
- Polyomavirus and related diseases
- vaccines and immunoinformatics approaches
- HIV Research and Treatment
Broad Institute
2021-2025
Dana-Farber Cancer Institute
2021-2025
Harvard University
2021-2025
Massachusetts Institute of Technology
2021
Massachusetts General Hospital
2020
University of Cincinnati
2018
New York University
2009-2016
New York Proton Center
2015
Institute of Biomedical Science
2015
In the past decade, social capital has been explored internationally in disaster and work literature, particularly terms of historical oppression limited economic resources disadvantaged communities. Social United States, however, had less integration. Using a qualitative grounded theory approach, we examine different types (bonding, bridging, linking) through lens. We how operated lives 40 families following Hurricane Katrina New Orleans, Louisiana. attempt to understand residents utilized...
Polymorphisms in the essential autophagy gene Atg16L1 have been linked with susceptibility to Crohn's disease, a major type of inflammatory bowel disease (IBD). Although inability control intestinal bacteria is thought underlie IBD, role during extracellular bacterial infections has not sufficiently examined and compared function other IBD genes, such as Nod2, which encodes cytosolic sensor. We find that mutant mice are resistant induced by model pathogen Citrobacter rodentium. An deficiency...
Abstract Single-cell transcriptomics has become the definitive method for classifying cell types and states, can be augmented with genotype information to improve lineage identification. Due constraints of short-read sequencing, current methods detect natural genetic barcodes often require cumbersome primer panels early commitment targets. Here we devise a flexible long-read sequencing workflow analysis pipeline, termed nanoranger , that starts from intermediate single-cell cDNA libraries...
The success of Mycobacterium tuberculosis (Mtb) as a pathogen rests upon its ability to grow intracellularly in macrophages. Interferon-gamma (IFN-γ) is critical host defense against Mtb and stimulates macrophage clearance through an autophagy pathway. Here we show that the protein ubiquilin 1 (UBQLN1) promotes IFN-γ-mediated autophagic Mtb. Ubiquilin family members have previously been shown recognize proteins aggregate neurodegenerative disorders. We find UBQLN1 can interact with surface...
To elucidate mechanisms by which T cells eliminate leukemia, we study donor lymphocyte infusion (DLI), an established immunotherapy for relapsed leukemia. We model cell dynamics integrating longitudinal, multimodal data from 94,517 bone marrow-derived single transcriptomes in addition to chromatin accessibility and receptor sequencing patients undergoing DLI. find that responsive tumors are defined enrichment of late-differentiated before DLI rapid, durable expansion early differentiated...
The ability of posttransplant cyclophosphamide (PTCY) to facilitate haploidentical transplantation has spurred interest in whether PTCY can improve clinical outcomes patients with HLA-matched unrelated donors undergoing peripheral blood stem cell (PBSCT). We investigated our institutional experience using PTCY-based graft-versus-host disease (GVHD) prophylaxis compared conventional tacrolimus-based regimens. overall survival, progression-free survival (PFS), relapse, nonrelapse mortality,...
Allogeneic hematopoietic stem cell transplantation (HSCT) is a potentially curative therapy for many hematologic malignancies as well non-malignant conditions. Part of the basis underlying HSCT relies upon induction graft versus leukemia (GVL) effect in which donor immune cells recognize and eliminate residual malignant within recipient, thereby maintaining remission. GVL clinically evident phenomenon; however, specific types responsible inducing this molecular mechanisms involved remain...
Understanding how intratumoral immune populations coordinate antitumor responses after therapy can guide treatment prioritization. We systematically analyzed an established immunotherapy, donor lymphocyte infusion (DLI), by assessing 348,905 single-cell transcriptomes from 74 longitudinal bone marrow samples of 25 patients with relapsed leukemia; a subset was evaluated both protein- and transcriptome-based spatial analysis. In acute myeloid leukemia (AML) DLI responders, we identified...
Journal Article Unravelling Social Capital: Disentangling a Concept for Work Get access Robert L. Hawkins, Hawkins * holds junior endowed chair as the McSilver Assistant Professor in Poverty Studies at Silver School of New York University. He is also affiliated with Institute on Policy & Research School. Katherine Maurer doctoral student University and Fellow Research. *Correspondence to Work, University, 1 Washington Square North, York, NY 10003, USA. E-mail: Robert.hawkins@nyu.edu Search...
Abstract We sought to evaluate the impact of cryopreservation unrelated donor (URD) peripheral blood stem cell (PBSC) grafts on engraftment, chimerism, and immune reconstitution in context COVID-19 pandemic. reviewed product characteristics clinical outcomes 101 patients receiving cryopreserved PBSCs from URDs between January 1, 2019 31 December, 2020, compared with 203 fresh URD PBSCs. observed no differences 6-month overall survival, progression-free or nonrelapse mortality. Patients had...
Abstract The novel coronavirus severe acute respiratory syndrome 2 (SARS-CoV-2), identified in late 2019 as the causative agent of COVID-19, was declared a pandemic by World Health Organization on 11 March 2020. Widespread community transmission United States triggered nationwide shutdown, raising major challenges for administration hematopoietic stem cell transplant (HSCT) and chimeric antigen receptor (CAR)-T therapies, leading many centers to delay or cancel operations. We sought assess...
Summary Understanding how intra-tumoral immune populations coordinate to generate anti-tumor responses following therapy can guide precise treatment prioritization. We performed systematic dissection of an established adoptive cellular therapy, donor lymphocyte infusion (DLI), by analyzing 348,905 single-cell transcriptomes from 74 longitudinal bone-marrow samples 25 patients with relapsed myeloid leukemia; a subset was evaluated protein-based spatial analysis. In acute myelogenous leukemia...
Characterizing cell–cell communication and tracking its variability over time are crucial for understanding the coordination of biological processes mediating normal development, disease progression, responses to perturbations such as therapies. Existing tools fail capture time-dependent intercellular interactions primarily rely on databases compiled from limited contexts. We introduce DIISCO, a Bayesian framework designed characterize temporal dynamics cellular using single-cell...
Defense strategies against infectious threats can be divided into resistance and tolerance mechanisms. Resistance mechanisms involve reduction of pathogen burden include many established examples, one them being the destruction intracellular pathogens through autophagy (xenophagy). Tolerance protect host from damage caused by or immune response independent load. The role in maintaining homeostasis to environmental stress suggests that this pathway is involved a variety agents. However,...
Mutations that alter virulence and antibiotic susceptibility arise persist during Staphylococcus aureus bacteremia. However, an experimental system demonstrating transmission following bacteremia has been lacking, thus implications of within-host adaptation for between-host are unknown. We report S. disseminates to the gastrointestinal tract mice intravenous injection readily transmits cohoused naive mice. Both intestinal dissemination were linked production factors based on gene deletion...