A5085594224- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Vector-Borne Animal Diseases
- Animal Nutrition and Physiology
- Salmonella and Campylobacter epidemiology
- Food Safety and Hygiene
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Advanced biosensing and bioanalysis techniques
- Advanced Numerical Analysis Techniques
- Listeria monocytogenes in Food Safety
- Respiratory viral infections research
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- interferon and immune responses
- Coccidia and coccidiosis research
- Diagnosis and Treatment of Venous Diseases
- Cardiovascular Effects of Exercise
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Muscle Physiology and Disorders
- Liver Disease Diagnosis and Treatment
- Autism Spectrum Disorder Research
Jordan University of Science and Technology
2007-2024
Lancaster University
2020-2024
University Hospitals of Cleveland
2023
University of Toledo
2013
University of Veterinary Medicine
2008
University of Jordan
2005
H9N2 avian influenza A viruses are endemic in poultry of many Eurasian countries and have caused repeated human infections Asia since 1998. To evaluate the potential threat to humans, we investigated replication transmission efficiency ferret model. Five wild-type (WT) viruses, isolated from different species 1988 through 2003, were tested vivo found replicate ferrets. However these achieved mild peak viral titers nasal washes when compared those observed with a H3N2 virus. Two transmitted...
Fertile eggs were obtained from three different broiler breeder flocks with levels of virus neutralizing antibodies to infectious bursal disease virus. Egg yolk these was tested for antibody titers by the neutralization test. Flock I had no antibodies, flock II medium level (1:200–1600; geometric mean = 1:975), and III a high (1:1600–6400; 1:3365). Chicks above challenged each 102 50% embryo infective dose IN serotype 1 variant at 1, 2, 4 wk age examined 5 11 days postchallenge. The average...
Until vaccines and effective therapeutics become available, the practical solution to transit safely out of current coronavirus disease 19 (CoVID-19) lockdown may include implementation an testing, tracing tracking system. However, this requires a reliable clinically validated diagnostic platform for sensitive specific identification SARS-CoV-2. Here, we report on development de novo, high-resolution comparative genomics guided reverse-transcribed loop-mediated isothermal amplification...
1. Abstract The triple reassortant H3N2 viruses were isolated for the first time from pigs in 1998 and are known to be endemic swine turkey populations United States. In 2004, we two breeder flocks Ohio Illinois. Infected hens showed no clinical signs, but experienced a complete cessation of egg production. this study, evaluated three isolates origin one isolate their transmission between turkeys. Although all 4 tested share high genetic similarity 8 genes, only strain...
This study was conducted to evaluate the rate of antibody transfer on a flock basis from hens their day-old chicks in meat-type chickens raised commercial setting. Fifteen randomly selected broiler-breeder were bled at 37, 40, and 45 wk age. At day bleeding, collected eggs identified tracked through hatching where 30 hatchlings sampled jugular vein. Antibodies against 10 different pathogens quantified serum samples, percentage maternal antibodies calculated chick titer divided by hen titer....
The effects of thermal manipulation (TM) at 38.5°C and 40°C for 6 h embryonic day (ED) 16, 9 ED 17, 12 18 on body weight (BW) cloacal temperature (Tb) during the first wk later post-hatch d 10, 14, 21, 28, 42 were evaluated. Furthermore, chicks' ability to cope with a challenge (TC; 41°C h) 14 was also A chick's response TC measured by determining temperature; plasma thyroid hormones (thyroxin (T4) triiodothyronine (T3)); packed cell volume (PCV); heterophil (H), lymphocyte (L), monocyte,...
Reported here are the effects of added formic acid on inhibitory effect Salmonella gallinarum in poultry feed.Two experiments were conducted to investigate viability S. and pH feed using different dietary levels (0.0, 0.5, 1.0 1.5%) broiler feed.Experiment one was artificially contaminated diet at 0, 1, 3, 5 7 days after treatment vitro.Formic showed a significant (p<0.05)reduction for all treatments with time treatment.Various vitro reduction depending upon concentration treated acid,...
An investigation was conducted in specific-pathogen-free chickens on the pathogenicity of bursa-derived and tissue culture-attenuated classic (STC) variant (IN) serotype 1 strains infectious bursal disease virus. The IN virus caused inflammation, necrosis, atrophy earlier than STC Both viruses lost their after four passages BGM-70 cells. A statistically significant level (P < 0.05) protection observed SPF vaccinated with attenuated used as a live or inactivated vaccine followed by homologous...
During the 1990s, several outbreaks of avian influenza (AI), caused by viruses H9N2 subtype, were reported in poultry various parts world. Currently, this infection seems to be endemic Middle and Far East, extensive circulation represents a risk factor for humans, because subtype have been sporadically introduced into human population. Little is known about gene constellation that are currently circulating East; thus, sequences eight IA isolated Jordan 2003 from analyzed. The results...
Thermal manipulation (TM) during broiler chicken embryogenesis has been shown to promote muscle development and growth. However, the molecular bases of promoting growth are not fully understood. The aim this study was investigate in chickens subjected TM. This included investigating changes mRNA expression levels marker genes, namely MyoD, myogenin, paired box transcription factor (Pax7) proliferating cell nuclear antigen (PCNA), factors insulin-like 1 (IGF-1), myostatin hormone (GH) on...
Mammalian cells utilize a wide spectrum of pathways to antagonize the viral replication. These are typically regulated by antiviral proteins and can be constitutively expressed but also exacerbated interferon induction. A myriad interferon-stimulated genes (ISGs) have been identified in mounting broad-spectrum responses. Members interferon-induced transmembrane (IFITM) family unique among these ISGs due their ability prevent virus entry through lipid bilayer into cell. In current study, we...
The global distribution of avian respiratory viruses highlights the need for effective surveillance programs and international collaboration to monitor viral circulation implement timely control measures. In current study, we aim provide a comprehensive overview infections in poultry flocks, focusing on major involved, their epidemiology, clinical manifestations, evolution based viroinformatics that will be helpful improve diagnostic methods, strategies including vaccines region. this...
Infectious bursal disease virus is a contagious, immunosuppressive of young chickens that controlled by vaccination. Cross-protection occurs between different strains the as result shared neutralizing epitopes. However, interactions two antigenically similar (a mild and pathogenic) coinfecting same host have not been investigated. Groups specific-pathogen-free were inoculated with strain followed pathogenic at 0, 16, 24, or 48 hr postinoculation (PI) strain. Virus persistence predominant...
Fresh eggshells collected from a local farm were subjected to different levels of surface contamination with feces containing (3 5 log₁₀) Escherichia coli O157:H7 or Staphylococcus aureus and incubated at 3 temperatures (10, 25, 32 °C). The penetration rates contaminating bacteria followed throughout the incubation period by tracing bacterial presence in shell, shell membranes, albumen, yolk. study revealed ability both E. enterotoxigenic S. grow on feces, penetrate move multiply within egg...
Abstract Until vaccines and effective therapeutics become available, the practical way to transit safely out of current lockdown may include implementation an testing, tracing tracking system. However, this requires a reliable clinically validated diagnostic platform for sensitive specific identification SARS-CoV-2. Here, we report on development de novo , high-resolution comparative genomics guided reverse-transcribed loop-mediated isothermal amplification (LAMP) assay. To further enhance...
Abstract Campylobacter jejuni is an important pathogen of significant public health importance. This associated with human infection and has been isolated from mammals birds. Ninety‐two cloacal C. isolates were identified 35 layer farms in Northern Jordan. Antimicrobial susceptibility was determined using minimal inhibitory concentration (MIC) disc diffusion techniques variable suggested breakpoints. Using MIC EUCAST cut‐off values, the study revealed a significantly high resistance level...
The highly pathogenic avian influenza H5N1 pandemic motivated countries around the World to be prepared for outbreaks within their borders. Well ahead of outbreak, in Jordan contingency plans were involving all relevant bodies both private and public sectors a practice was simulated test effectiveness task force applicability plan. virus detected confirmed one turkey backyard flock comprising 12 turkeys 25 chickens Kufranjah, 6 km from Ajloun city, on 23 March 2006. samples by following day....