A5055336708- Plant tissue culture and regeneration
- Seed Germination and Physiology
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant Taxonomy and Phylogenetics
- Biochemical Analysis and Sensing Techniques
- Plant Pathogens and Fungal Diseases
- Enzyme Production and Characterization
- Plant responses to water stress
- Plant Genetic and Mutation Studies
- Polysaccharides Composition and Applications
- Flowering Plant Growth and Cultivation
- Chromosomal and Genetic Variations
- Reproductive Biology and Fertility
- Plant Growth Enhancement Techniques
- Proteins in Food Systems
- Turfgrass Adaptation and Management
- Tissue Engineering and Regenerative Medicine
- Biochemical and Structural Characterization
- Plant Ecology and Taxonomy Studies
- Plant responses to elevated CO2
- Plant Gene Expression Analysis
- Botanical Research and Applications
Jagiellonian University
2013-2024
University of Bonn
2010
The formation of an extracellular matrix surface network (ECMSN), and associated changes in the distribution arabinogalactan-protein pectin epitopes, have been studied during somatic embryogenesis (SE) callogenesis Trifolium nigrescens Viv. Scanning electron microscopy observations revealed occurrence ECMSN on cotyledonary-staged embryos as well peripheral, non-regenerating callus cells. six AGP (JIM4, JIM8, JIM13, JIM16, LM2, MAC207) four (JIM5, JIM7, LM5, LM6) epitopes was analysed early...
Androgenesis-based methods of doubled haploid (DH) production show considerable variation in efficiency different barley genotypes. Arabinogalactan proteins (AGPs) have been shown to play a key role several developmental processes, including embryogenesis, plant species. In this study we investigated the effect exogenous AGPs from gum arabic on androgenesis and regeneration anther culture. Supplementation induction medium with 10 mg l−1 increased total rate up 2.8 times; when exposure GA was...
This work demonstrated how reactive oxygen species (ROS) are involved in the regulation of rhizogenesis from hypocotyls Mesembryanthemum crystallinum L. cultured on a medium containing 1-naphthaleneacetic acid (NAA). The increase NADPH oxidase activity was correlated with an hydrogen peroxide (H2O2) content and induction mitotic vascular cylinder cells, leading to root formation hypocotyls. Diphenylene iodonium (DPI), inhibitor oxidase, inhibited H2O2 production blocked rhizogenesis....
The aim of this study was to identify and examine the expression pattern ortholog SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE gene from Trifolium nigrescens (TnSERK) in embryogenic non-regenerative cultures immature cotyledonary-stage zygotic embryos (CsZEs). In presence 1-naphthaleneacetic acid N(6)-[2-isopentenyl]-adenine, CsZE regenerated embryoids directly a lengthy culture produced callus which or remained non-regenerative. As revealed by semi-quantitative reverse transcription...
Microscopy and flow cytometry (FCM) were used to study somatic embryogenesis (SE) from zygotic embryos of Trifolium nigrescens Viv. determine if there any relationships between characteristics (morphology, anatomy, genome size stability) their regenerability. Embryoids induced on Murashige Skoog (MS) medium containing 4 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) 2 N6-[2-isopentenyl]-adenine (2iP) either directly hypocotyls or via an intervening callus, depending the duration culture. The...
H2O2 is necessary to elicit rhizogenic action of auxin. Activities specific catalase and manganese superoxide dismutase forms mark roots development. Hypocotyl explants Mesembryanthemum crystallinum regenerated on medium containing 2,4-dichlorophenoxyacetic acid. Explants became competent respond the auxin day 3 culture, when hydrogen peroxide content in cultured tissue was highest. L-Ascorbic acid added at 5 μM lowered level, inhibited rhizogenesis induced non-regenerative callus,...
A comparison of the hydrogen peroxide (H2O2) content, proline and betacyanin concentration activities some antioxidant enzymes (catalase, superoxide dismutase, guaiacol ascorbate peroxidases) was made in Mesembryanthemum crystallinum L. calli differing rhizogenic potential. Callus induced from hypocotyls 10-day-old seedlings on a medium containing 1 mg l−1 2,4-dichlorophenoxyacetic acid 0.2 kinetin, which either supplemented with 40 mM NaCl (CIM-NaCl medium) or did not contain any salt (CIM...
<em>Helianthus tuberosus</em> is economically important species. To improve characters of this energetic plant via genetic modification, production callus tissue and regeneration are the first steps. A new, potentially cultivar Albik was used in study to test induction regeneration. Callus produced on leaves, petioles, apical meristems stems from field-harvested plants but totally non-morphogenic. Its started cortex vascular bundles as confirmed by histological analysis. The...
Abstract This study investigated the antioxidant activity of gum Arabic (GA) during androgenesis in barley anthers. After stress pretreatment anthers were cultured presence 10 mg L −1 GA (Gm) or on control medium (Cm) and compared with respect to selected respiratory enzymes endogenous hydrogen peroxide (H 2 O ) content. The from Cm Gm differed strategy against H overproduction, that total peroxidase (POX) number POX isoforms significantly higher Gm- than Cm-cultured material. High...
Whole plant regeneration via organogenesis in immature embryo culture of <em>Trifolium michelianum</em> Savi. was obtained. The shoots were induced directly from the hypocotyl or indirectly through callus on EC6 medium supplemented with 0.5 and 5 mg/l BAP, respectively. Sporadically formation preceded by shoot regeneration. Both obtained regenerated into plants when subcultured half-strength Murashige Skoog (MS) medium, free growth regulators. Histological observations revealed...
The enrichment with antioxidants (glutathione or ascorbate) prooxidants (alloxan, methylviologen, hydrogen peroxide) of root inducing medium significantly decreased rhizogenesis frequency inhibited roots regeneration (ascorbate, methylviologen) during the in vitro culture Mesembryanthemum crystallinum L. hypocotyls. adventitious morphology, hairs length and density, was also influenced. Changes course were related to differences peroxide concentration following days between explants...
Proteins belonging into cryptochrome/photolyase family act as either blue light photoreceptors or enzymes responsible for repair of pyrimidine dimers produced under UV. Surprisingly, the members plant-specific photolyase/blue-light receptor 2 (PPL/PHR2) subclade this family, are almost completely uncharacterized. Here, we focused on physiological role protein encoded by Arabidopsis At2g47590 gene. Our results demonstrate first time its crucial in cotyledon greening and maintenance plant...