A5077460068- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Parvovirus B19 Infection Studies
- Neonatal Health and Biochemistry
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Viral-associated cancers and disorders
- Adenosine and Purinergic Signaling
- Polyomavirus and related diseases
- Bacillus and Francisella bacterial research
- Burn Injury Management and Outcomes
- Drug-Induced Ocular Toxicity
- Diabetes and associated disorders
- Immune Cell Function and Interaction
- Bacterial biofilms and quorum sensing
- Biosensors and Analytical Detection
- Ophthalmology and Eye Disorders
- Cerebral Venous Sinus Thrombosis
- Vestibular and auditory disorders
- Immune Response and Inflammation
- Hearing, Cochlea, Tinnitus, Genetics
- Neutropenia and Cancer Infections
- Virology and Viral Diseases
- Medical Device Sterilization and Disinfection
- Kawasaki Disease and Coronary Complications
- Toxoplasma gondii Research Studies
Asahikawa Medical University
2010-2017
Gifu Pharmaceutical University
2017
Asahikawa Medical College Hospital
1996-2010
Centers for Disease Control and Prevention
2003-2004
Obihiro National Hospital
2004
Nayoro City General Hospital
2004
National Institute of Infectious Diseases
2004
Fukushima Medical University
2004
Emory University
2003
Winship Cancer Institute
2003
Background As congenital cytomegalovirus (CMV) infection causes significant clinical consequences not only at birth but also later as neurological sequelae, it is critical to establish a strategy for screening congenitally infected newborns. Previous studies have identified an insufficient sensitivity in methods based on the use of dried blood spots (DBSs). Objectives To evaluate feasibility authors' recently developed method large-scale CMV and identify risk factors infection. Methods More...
Sensorineural hearing loss (SNHL) is the most common congenital disease. Longitudinal studies of infants with cytomegalovirus (CMV) infection have demonstrated an association between CMV and SNHL. However, because lack suitable neonatally collected specimens, proportion CMV-associated SNHL has not been defined, nor relationship major genetic causes SNHL, such as mutations in GJB2 gene.Sixty-seven children severe were analyzed for human herpesvirus 6 (HHV-6) infections mutations. DNA...
Glycoproteins M (gM) and N (gN) are well conserved across the herpesvirus family their involvement in virus penetration egress is described, especially for alphaherpesviruses. Because there was no previous study on homologues of human 8 glycoproteins (gM8) (gN8), we analysed biochemical functional characteristics. We found that: (i) gM8 aggregated following heat treatment; (ii) a virion component; (iii) gN8 were N-glycosylated; (iv) formed specific complex with gN8; (v) required processing...
ABSTRACT To analyze the mechanisms of entry human herpesvirus 8 (HHV-8), we established a reporter cell line T1H6 that contains lac Z gene under control polyadenylated nuclear RNA promoter, known to be strongly activated by viral transactivator, Rta. We found infection with cell-free virus, as well cocultivation HHV-8-positive primary effusion lymphoma lines, in sensitive and dose-dependent manner. Addition Polybrene centrifugation enhanced, but polysulfonate compounds inhibited, HHV-8...
Cytomegalovirus (CMV) is the most common cause of congenital virus infection. However, risk factors for infection in utero and progression to a severe clinical outcome remain uncertain. Recent studies have identified associations specific single nucleotide polymorphisms (SNPs) Toll-like receptor (TLR) genes with susceptibility infections some viruses their outcome.Genetic TLR-2, TLR-4, TLR-9 were analyzed 87 children CMV by TaqMan allelic discrimination assay. The frequencies genotypes...
Investigation of sequence polymorphisms in the glycoprotein N (gN; gp4273), gO (gp4274) and gH (gp4275) genes human cytomegalovirus (HCMV) strains collected from 63 Japanese children revealed that their genotype distribution differed slightly Caucasian populations there was a significant linkage between gN genotypes. Linkage these genotypes obtained has been reported, so our similar findings infants are consistent with this, suggest generality this linkage. Sequence analysis suggests...
To clarify the impact of congenital cytomegalovirus (CMV) infection on developmental disabilities, 20 children with disabilities unknown cause were analyzed.Five CMV positive and had no clinical manifestations at birth.Intracranial calcification was observed in 4 cases.Thus, is a significant disabilities.Cytomegalovirus one most common causes intrauterine infection.The prevalence ranges from 0.3% to 2.4% live births developed countries.Approximately 10% infected neonates are symptomatic...
Although the use of dried blood spots has been proposed for screening newborns with congenital cytomegalovirus infection, viral loads in were significantly smaller than those urine (P < 0.001), and DNA recovery from using thermal shock procedure was inefficient. In contrast, our urine-based program identified asymptomatic cases low blood.
Since congenital cytomegalovirus (CMV) infection causes late-onset sequelae, the identification of CMV-infected newborns is important. For this purpose, we established a simple real-time PCR assay using filter disk. Combined with collection urine papers placed in diaper, can make CMV screening more feasible and cost-effective.
Abstract Background The aim of this study was to evaluate the outcome congenital cytomegalovirus ( CMV ) infection identified on urine‐filter screening assay at >2 years’ follow up, and observe clinical outcomes after anti‐ treatment. Methods Sixty 72 patients were enrolled clinically observed for years. Forty‐three asymptomatic birth; seven symptomatic birth but untreated with drugs; 10 treated drugs. Results Of 43 patients, three developed hearing loss or language disability which...
To evaluate the parameters associated with significant gastrointestinal (GI) involvement in Henoch-Schönlein Purpura (HSP), and construct a scoring system for identification of patients at high risk gross blood stools. Data HSP hospitalized each seven institutes were retrospectively analyzed. Patients divided into four groups according to consequent severity GI involvement. Identification laboratory time admission then used differentiate groups, predict intestinal bleeding was constructed....
The inhibitory activities of acyclovir (ACV), 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil (BV-araU), ganciclovir (GCV), 9-(2-deoxy-2-hydroxymethyl-beta-D-erythro-oxetanosyl)guanine (OXT-G), and (+)-9-[(1R,2R,3S)-2,3-bis(hydroxymethyl)Cyclobutyl]guanine (cOXT-G) on the replication wild-type thymidine kinase (TK)-negative strains herpes simplex virus types 1 2 varicella-zoster (VZV) strain human cytomegalovirus were tested to clarity whether phosphorylation these compounds is catalyzed...
Most causative organisms of sepsis in immunocompromised patients are the same species as those that colonize their own nasopharynx or intestinal tract. To determine whether strains recovered from blood originate mainly patients' flora, isolates and throat and/or stool were investigated by genomic analyses. Surveillance cultures taken prospectively cancer being treated with intensive chemotherapy followed haematopoietic stem-cell transplantation. In cases which isolate was stool, profiles...
ABSTRACT To simplify the detection of infectious human cytomegalovirus (HCMV), we generated a cell line that produced luciferase in dose-dependent manner upon HCMV infection. Using this line, identified anti-HCMV compounds from diverse library 9,600 compounds. One them, 1-(3,5-dichloro-4-pyridyl)piperidine-4-carboxamide (DPPC), was effective against (Towne strain) infection lung fibroblast cells at 50% concentration 2.5 μM. DPPC also inhibited growth clinical isolates and guinea pig mouse...
Abstract The virulence of thymidine kinase‐negative herpes simplex virus type 1 (HSV‐1; VRTK − strain) and 2 (HSV‐2; UWTK was studied in comparison with that their parental strains (VR‐3 UW‐268, respectively) an encephalitis model adult (4‐week‐old) newborn (3‐day‐old) mice. Viral kinase (TK) activity essential for the maximum expression HSV‐1, because 50% lethal dose (LD 50 ) 60 times higher than VR‐3 brains mice expressing high levels cellular TK activity. However, strain showed same as...
To investigate reinfection in patients with congenital cytomegalovirus (CMV) infection, we established a CMV subtype-specific real-time quantitative PCR method targeting the gH epitope region that can be used for evaluating pathogenic strains cases of mixed infection.