A5032104718- Monoclonal and Polyclonal Antibodies Research
- Hepatitis C virus research
- Viral Infectious Diseases and Gene Expression in Insects
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Transgenic Plants and Applications
- Protein purification and stability
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- HIV Research and Treatment
- Respiratory viral infections research
- Diabetes and associated disorders
- Galectins and Cancer Biology
- Vector-Borne Animal Diseases
- Dermatology and Skin Diseases
- Helicobacter pylori-related gastroenterology studies
- Immunodeficiency and Autoimmune Disorders
- Viral Infections and Vectors
- Liver Disease and Transplantation
- Microbial Inactivation Methods
- Smart Grid and Power Systems
Karolinska Institutet
2006-2023
Karolinska University Hospital
2006-2023
Hepatitis C virus (HCV), a major cause of chronic liver disease in humans, is the focus intense research efforts worldwide. Yet structural data on viral envelope glycoproteins E1 and E2 are scarce, spite their essential role life cycle. To obtain more information, we developed an efficient production system recombinant ectodomain (E2e), truncated immediately upstream its trans-membrane (TM) region, using Drosophila melanogaster cells. This yields majority monomeric protein, which can be...
ABSTRACT Chikungunya virus (CHIKV) is a reemerging mosquito-borne alphavirus that has caused severe epidemics in Africa and Asia occasionally Europe. As of today, there no licensed vaccine available to prevent CHIKV infection. Here we describe the development evaluation novel candidates were attenuated by deleting large part gene encoding nsP3 or entire 6K administered as viral particles infectious genomes launched DNA. The resulting mutants genetically stable elicited high magnitudes...
One way to dissect the antibody response an invading microorganism is clone repertoire from immune donors and subsequently characterize specific antibodies. Recently, methodological advances have allowed investigations of neutralizing antibodies against hepatitis C virus (HCV) in vitro . We investigated three human mAbs, previously isolated individual infected with HCV genotype 2b, that are known cross-react a binding assay envelope E2 protein genotypes 1a 1b. now report two them activity...
RNA-based vaccines represent an interesting immunization modality, but suffer from poor stability and a lack of efficient clinically feasible delivery technologies. This study evaluates the immunogenic potential naked in vitro transcribed Semliki Forest virus replicon RNA (RREP) delivered intradermally combination with electroporation. Replicon-immunized mice showed strong cellular humoral response, contrary to immunized regular mRNA. RREP-elicited induction interferon-γ secreting CD8+ T...
Abstract The threat of emergence further SARS-CoV-2 variants, and the future spillover potential other sarbecoviruses has prompted continued efforts to isolate broadly reactive monoclonal antibodies for therapeutic use. In this study, we generated from immunised cattle, primarily because their ability produce with ultra-long heavy chain complementarity determining region 3 (CDRH3) domains. Such have been shown potent cross-reactive neutralisation phenotypes in virus infections. Following...
ABSTRACT Vaccination using “naked” DNA is a highly attractive strategy for induction of pathogen-specific immune responses; however, it has been only weakly immunogenic in humans. Previously, we constructed DNA-launched Semliki Forest virus replicons (DREP), which stimulate pattern recognition receptors and induce augmented responses. Also, vivo electroporation was shown to enhance responses induced by conventional vaccines. Here, combine these two approaches show that increases CD8 + T cell...
Abstract Immunological and inflammatory reactions have been suggested to a role in the development of schizophrenia, hypothesis that has recently supported by genetic data. The aim our study was perform an unbiased search for autoantibodies patients with first psychotic episode, explore association between any seroreactivity Diagnostic Statistical Manual Mental Disorders, fourth edition (DSM-IV) disorder characterized chronic or relapsing symptoms. We collected plasma samples from 53 when...
Fab molecules are used as therapeutic agents, and invaluable tools in structural biology. We report here a method for production of recombinant Drosophila S2 cells use Stably transfected cell lines expressing the were created within weeks. The was secreted, after affinity size exclusion chromatography, 16 mg pure protein obtained from liter culture. functional formed complex with its cognate antigen demonstrated by co-precipitation chromatography. Biochemical characterization indicated that...
Pancreas disease (PD) of Atlantic salmon is an emerging caused by Salmonid alphavirus (SAV) which mainly affects salmonid aquaculture in Western Europe. Although genome structure SAV has been characterized and each individual viral protein identified, the role 6K replication infectivity remains undefined. The alphaviruses a small hydrophobic involved membrane permeabilization, processing virus budding. Because these common features are shared across many species, they have named viroporins....
Ligands of pattern recognition receptors (PRRs) including Toll-like (TLRs) stimulate innate and adaptive immune responses are considered as potent adjuvants. Combinations ligands might act in synergy to induce stronger broader compared stand-alone ligands. Alphaviruses endosomal TLRs 3, 7 8 well the cytoplasmic PRR MDA-5, resulting induction a strong type I interferon (IFN) response. Bacterial flagellin stimulates TLR5 when delivered intracellularly cytosolic NLRC4, leading secretion...
Eliciting effective immune responses using non-living/replicating DNA vaccines is a significant challenge. We have previously shown that ballistic dermal plasmid DNA-encoded flagellin (FliC) promotes humoral as well cellular immunity to co-delivered antigens. Here, we observe encoding secreted FliC (pFliC(-gly)) produces capable of activating two innate receptors known detect flagellin; Toll-like Receptor 5 (TLR5) and Nod-like family CARD domain-containing protein 4 (NRLC4). To test the...
A salmonid alphavirus (SAV) replicon has been developed to express heterologous antigens but protein production was low modest compared with terrestrial replicons. In this study, we have several modifications a SAV construct and analysed their influence on foreign gene expression. We found that an insertion of translational enhancer consisting the N-terminal 102 nt capsid gene, together nucleotide sequence encoding foot-and-mouth disease virus (FMDV) 2A peptide, caused significant increase...
The majority of the world population carry gastric pathogen
Immunological reactions may have a role in subgroups of patients suffering from psychiatric disorders. Possible markers for such be autoantibodies currently unknown nature. If identified, they could indicate which that would benefit immunomodulatory treatment addition to standard interventions. Modern proteomic methods allow analyses antibody binding thousands different human proteins, facilitating the identification undiscovered autoantibodies. We explored association between any...