A5004226873- Heme Oxygenase-1 and Carbon Monoxide
- Platelet Disorders and Treatments
- Neonatal Health and Biochemistry
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Blood Coagulation and Thrombosis Mechanisms
- Erythrocyte Function and Pathophysiology
- Alcohol Consumption and Health Effects
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Eosinophilic Disorders and Syndromes
- COVID-19 Clinical Research Studies
- Kruppel-like factors research
- Mast cells and histamine
- Healthcare Policy and Management
- Venous Thromboembolism Diagnosis and Management
- COVID-19 and healthcare impacts
- Chronic Disease Management Strategies
- Antiplatelet Therapy and Cardiovascular Diseases
- Healthcare Systems and Technology
- Lipid metabolism and disorders
- Hemoglobinopathies and Related Disorders
Medical College of Wisconsin
2023
Versiti Blood Center of Wisconsin
2023
Stony Brook University
2015-2022
Children's Health Ireland at Crumlin
2022
State University of New York
2018
Biliverdin IXβ reductase (BLVRB) is an NADPH-dependent enzyme previously implicated in a redox-regulated mechanism of thrombopoiesis distinct from the thrombopoietin (TPO)/c-MPL axis. Here, we apply computational modeling to inform molecule design, followed by de novo syntheses and screening unique small molecules retaining capacity for selective BLVRB inhibition as novel platelet-enhancing strategy. Two classes are identified, NMR spectroscopy co-crystallization studies confirm binding...
Despite the transient hyporeactivity of neonatal platelets, full-term neonates do not display a bleeding tendency, suggesting potential compensatory mechanisms which allow for balanced and efficient hemostasis. This study aimed to utilize small-volume, whole blood platelet functional assays assess response downstream hemostatic agonists thrombin adenosine diphosphate (ADP). Thrombin activates platelets via protease-activated receptors (PARs) 1 4, whereas ADP signals P2Y1 P2Y12 as positive...
Bioenergetic requirements of hematopoietic stem cells and pluripotent (PSCs) vary with lineage fate, cellular adaptations rely largely on substrate (glucose/glutamine) availability mitochondrial function to balance tricarboxylic acid (TCA)-derived anabolic redox-regulated antioxidant functions. Heme synthesis degradation converge in a linear pathway that utilizes TCA cycle-derived carbon cataplerotic reactions tetrapyrrole biosynthesis, terminated by NAD(P)H-dependent biliverdin reductases...
Inflammatory stimuli have divergent effects on peripheral platelet counts, although the mechanisms of thrombocytopenic and thrombocytotic responses remain poorly understood. A candidate gene approach targeting 326 polymorphic genes enriched in thrombopoietic cytokine signaling pathways was applied to identify single nucleotide variants (SNVs) implicated enhanced cohorts with reactive thrombocytosis (RT) or essential (myeloproliferative neoplasm [MPN]) (ET). Cytokine profiles incorporating a...