A5075353956- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Retinal Development and Disorders
- Photoacoustic and Ultrasonic Imaging
- Functional Brain Connectivity Studies
- Ultrasound and Hyperthermia Applications
- Neuroscience and Neuropharmacology Research
Inserm
2021-2023
Institut de la Vision
2021-2023
Centre National de la Recherche Scientifique
2021-2023
Sorbonne Université
2021-2023
Abstract Remote and precisely controlled activation of the brain is a fundamental challenge in development brain–machine interfaces for neurological treatments. Low-frequency ultrasound stimulation can be used to modulate neuronal activity deep brain, especially after expressing ultrasound-sensitive proteins. But so far, no study has described an ultrasound-mediated strategy whose spatiotemporal resolution acoustic intensity are compatible with mandatory needs interfaces, particularly visual...
After revolutionizing neuroscience, optogenetic therapy has entered successfully in clinical trials for restoring vision to blind people with degenerative eye diseases, such as retinitis pigmentosa. These still have evaluate the visual acuity achieved by patients and determine if it reaches its theoretical limit extrapolated from ex vivo experiments. Different strategies are developed parallel reduce required light levels improve information processing targeting various cell types. For loss...
To better understand how the brain allows primates to perform various sets of tasks, ability simultaneously record neural activity at multiple spatiotemporal scales is challenging but necessary. However, contribution single-unit activities (SUAs) neurovascular remains be fully understood. Here, we combine functional ultrasound imaging cerebral blood volume (CBV) and SUA recordings in visual fronto-medial cortices behaving macaques. We show that provides a significant estimate response below...
Over the last 15 years, optogenetics has changed fundamental research in neuroscience and is now reaching toward therapeutic applications. Vision restoration strategies using are at forefront of these new clinical opportunities. But applications to human patients suffering from retinal diseases leading blindness raise important concerns on long-term functional expression optogenes efficient signal transmission higher visual centers. Here, we demonstrate non-human primates continued...
Abstract Optogenetics has revolutionized neurosciences by allowing fine control of neuronal activity. An important aspect for this is assessing the activation and/or adjusting stimulation, which requires imaging entire volume optogenetically-induced ideal technique aim fUS imaging, allows one to generate brain-wide maps with submesoscopic spatial resolution. However, optical stimulation brain blue light might lead non-specific activations at high irradiances. optogenetic can be obtained...
Abstract Remote, precisely controlled activation of the brain is a fundamental challenge in development brain-machine interfaces providing feasible rehabilitation strategies for neurological disorders. Low-frequency ultrasound stimulation can be used to modulate neuronal activity deep 1–7 , but this approach lacks spatial resolution and cellular selectivity loads with high levels acoustic energy. The combination expression ultrasound-sensitive proteins (‘sonogenetic stimulation’) provide...
Optogenetic stimulation of the primary visual cortex (V1) is a promising therapy for sight restoration, but it remains unclear what total cerebral volume activated after surface stimulation. In this study, we expressed red-shifted opsin ChrimsonR in excitatory neurons within V1 rats, and used fine spatial resolution provided by functional ultrasound imaging (fUS) over whole depth brain to investigate response focal We observed optogenetic activation high proportion V1. Extracellular...
ABSTRACT To better understand how the brain allows primates to perform various set of tasks, ability simultaneously record activity at multiple temporal and spatial scales is challenging but necessary. In non-human primates, combined fMRI electrophysiological recordings have not disentangle contributions spiking neurovascular response. Here, we functional ultrasound imaging (fUS) cerebral blood volume (CBV) recording single-unit activities (SUA) in visual fronto-medial cortices behaving...
Abstract Over the last 15 years, optogenetics has changed fundamental research in neuroscience, and is now reaching toward therapeutic applications. Vision restoration strategies using are at forefront of these new clinical opportunities. But applications to human patients suffering from retinal diseases leading blindness rise important concerns on long-term functional expression optogenes efficient signal transmission higher visual centers. Here we demonstrate non-human primates, continued...