A5017756518- Neural dynamics and brain function
- Visual perception and processing mechanisms
- Neural and Behavioral Psychology Studies
- Neuroscience and Neural Engineering
- Photoacoustic and Ultrasonic Imaging
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Functional Brain Connectivity Studies
- EEG and Brain-Computer Interfaces
- Color perception and design
- Ultrasound Imaging and Elastography
- Olfactory and Sensory Function Studies
- Visual Attention and Saliency Detection
- Ultrasound and Hyperthermia Applications
- Face Recognition and Perception
- Gaze Tracking and Assistive Technology
- Advanced Memory and Neural Computing
- Optical Imaging and Spectroscopy Techniques
- Neuroscience and Neuropharmacology Research
- Color Science and Applications
Inserm
2018-2023
Sorbonne Université
2019-2023
Institut de la Vision
2019-2023
Centre National de la Recherche Scientifique
2009-2023
National Eye Institute
2014-2018
Institut du Cerveau
2018
University of California, Los Angeles
2010-2017
National Institutes of Health
2014
Centre de recherche cerveau et cognition
2007-2009
Université Toulouse III - Paul Sabatier
2009
Abstract Remote and precisely controlled activation of the brain is a fundamental challenge in development brain–machine interfaces for neurological treatments. Low-frequency ultrasound stimulation can be used to modulate neuronal activity deep brain, especially after expressing ultrasound-sensitive proteins. But so far, no study has described an ultrasound-mediated strategy whose spatiotemporal resolution acoustic intensity are compatible with mandatory needs interfaces, particularly visual...
Neuroimaging modalities such as MRI and EEG are able to record from the whole brain, but this comes at price of either limited spatiotemporal resolution or sensitivity. Here, we show that functional ultrasound imaging (fUS) brain is assess local changes in cerebral blood volume during cognitive tasks, with sufficient temporal measure directional propagation signals. In two macaques, observed an abrupt transient change supplementary eye field (SEF) activity when animals were required modify...
Abstract Vision restoration is an ideal medical application for optogenetics, because the eye provides direct optical access to retina stimulation. Optogenetic therapy could be used diseases involving photoreceptor degeneration, such as retinitis pigmentosa or age-related macular degeneration. We describe here selection, in non-human primates, of a specific optogenetic construct currently tested clinical trial. microbial opsin ChrimsonR, and showed that AAV2.7m8 vector had higher...
When exploring a visual scene, some objects perceptually popout because of difference color, shape, or size. This bottom-up information is an important part many models describing the allocation attention. It has been hypothesized that lateral intraparietal area (LIP) acts as "priority map," integrating and top-down to guide Despite large literature influences in LIP, presence pure salience response salient stimulus defined by its static features alone not reported. We compared LIP responses...
Deep regions of the brain are not easily accessible to investigation at mesoscale level in awake animals or humans. We have recently developed a functional ultrasound (fUS) technique that enables imaging hemodynamic responses visual tasks. Using fUS on two nonhuman primates performing passive fixation task, we constructed retinotopic maps depth cortex (V1, V2, and V3) calcarine lunate sulci. The could be acquired single-hour session with relatively few presentations stimuli. spatial...
In everyday life, we efficiently find objects in the world by moving our gaze from one location to another. The efficiency of this process is brought about ignoring items that are dissimilar target and remembering which target-like have already been examined. We trained two animals on a visual foraging task they had reward-loaded among five task-irrelevant distractors potential targets. found both performed efficiently, rarely examining particular twice. recorded single unit activity 54...
Since the late 2010s, Transcranial Ultrasound Stimulation (TUS) has been used experimentally to carryout safe, non-invasive stimulation of brain with better spatial resolution than Magnetic (TMS). This innovative method emerged as a novel and valuable device for studying function in humans animals. In particular, single pulses TUS directed oculomotor regions have shown modulate visuomotor behavior non-human primates during 100 ms ultrasound pulses. present study, sustained effect was induced...
To better understand how the brain allows primates to perform various sets of tasks, ability simultaneously record neural activity at multiple spatiotemporal scales is challenging but necessary. However, contribution single-unit activities (SUAs) neurovascular remains be fully understood. Here, we combine functional ultrasound imaging cerebral blood volume (CBV) and SUA recordings in visual fronto-medial cortices behaving macaques. We show that provides a significant estimate response below...
The shading of an object provides important cue for recognition, especially determining its 3D shape. However, neuronal mechanisms that allow the recovery shape from are poorly understood. aim our study was to determine basis coding in area V4 awake macaque monkey. We recorded responses cells stimuli presented parafoveally while monkeys fixated a central spot. used set made 8 different shapes illuminated 4 directions (from above, left, right and below) 2D controls each stimulus. results show...
Over the last 15 years, optogenetics has changed fundamental research in neuroscience and is now reaching toward therapeutic applications. Vision restoration strategies using are at forefront of these new clinical opportunities. But applications to human patients suffering from retinal diseases leading blindness raise important concerns on long-term functional expression optogenes efficient signal transmission higher visual centers. Here, we demonstrate non-human primates continued...
The basal ganglia are important for action selection. They also implicated in perceptual and cognitive functions that seem far removed from motor control. Here, we tested whether the role of selection extends to nonmotor aspects behavior by recording neuronal activity caudate nucleus while animals performed a covert spatial attention task. We found neurons strongly select location relevant stimulus throughout task even absence any overt action. This spatially selective was dependent on...
Recent work has implicated the primate basal ganglia in visual perception and attention, addition to their traditional role motor control. The ganglia, especially caudate nucleus ‘head’ (CDh) of striatum, receive indirect anatomical connections from superior colliculus (SC), a midbrain structure that is known play crucial control attention. To test possible functional relationship between these subcortical structures, we recorded CDh neuronal activity macaque monkeys before during unilateral...
Abstract Optogenetics has revolutionized neurosciences by allowing fine control of neuronal activity. An important aspect for this is assessing the activation and/or adjusting stimulation, which requires imaging entire volume optogenetically-induced ideal technique aim fUS imaging, allows one to generate brain-wide maps with submesoscopic spatial resolution. However, optical stimulation brain blue light might lead non-specific activations at high irradiances. optogenetic can be obtained...
The enhancement of neuronal responses in many visual areas while animals perform spatial attention tasks has widely been thought to be the neural correlate attention, but it is unclear whether presence or absence this modulation contributes our striking inability notice changes change blindness examples. We asked area V4 and lateral intraparietal (LIP) posterior parietal cortex could explain limited ability subjects attend multiple items a display. trained detection task which they had...
Abstract Restoring vision using optogenetics is an ideal medical application because the eye offers a direct window to access and stimulate pathological area: retina. Optogenetic therapy could be applied diseases with photoreceptor degeneration such as retinitis pigmentosa. Here, we select specific optogenetic construct that now used in clinical trial assess opsin functional efficacy on non-human primate’s retinal ganglion cells (RGCs). We chose microbial ChrimsonR showed vector AAV2.7m8...
In both human and nonhuman primates (NHP), the medial prefrontal region, defined as supplementary eye field (SEF), can indirectly influence behavior selection through modulation of primary process in oculomotor structures. To perform this control, SEF integrates multiple cognitive signals such attention, memory, reward, error. As changes pupil responses assess these efforts, a better understanding precise dynamics by which diameter cortex activity interact requires thorough investigations...
Abstract Remote, precisely controlled activation of the brain is a fundamental challenge in development brain-machine interfaces providing feasible rehabilitation strategies for neurological disorders. Low-frequency ultrasound stimulation can be used to modulate neuronal activity deep 1–7 , but this approach lacks spatial resolution and cellular selectivity loads with high levels acoustic energy. The combination expression ultrasound-sensitive proteins (‘sonogenetic stimulation’) provide...
When looking around at the world, we can only attend to a limited number of locations. The lateral intraparietal area (LIP) is thought play role in guiding both covert attention and eye movements. In this study, tested involvement LIP mechanisms with change detection task. task, animals had indicate whether an element changed during blank trial by making saccade it. If no changed, they maintain fixation. We examine how animal's behavior biased based on activity prior presentation stimulus...
Summary Deep regions of the brain are not easily accessible to investigation at mesoscale level in awake animals or humans. We have recently developed functional Ultrasound (fUS) imaging fUS technique uncover deep hemodynamic responses. Applying on two non-human primates performing a passive fixation task, we reconstructed their retinotopic maps down calcarine and lunate sulci visual areas (V1, V2 V3). These were acquired single hour session with very few stimuli presentation. The spatial...
ABSTRACT Pupil dilation is known to be an index of cognitive effort. Nevertheless, our lack knowledge the precise dynamics through which pupil size and activity medial prefrontal cortex are conjugated during tasks highlights need for its further investigation before, during, after changes in size. Here, we tested whether related supplementary eye field (SEF) a mixed pro/anti-saccade oculomotor task two macaque monkeys. We used functional ultrasound imaging (fUS) examine temporal brain at...