A5013652088- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- CCD and CMOS Imaging Sensors
- Ion channel regulation and function
- Crystallography and molecular interactions
- Cell Image Analysis Techniques
- Retinal Diseases and Treatments
- Neurogenesis and neuroplasticity mechanisms
- GABA and Rice Research
- Optical Imaging and Spectroscopy Techniques
- Molecular Communication and Nanonetworks
- Cardiac electrophysiology and arrhythmias
- Ultrasound and Hyperthermia Applications
- Visual perception and processing mechanisms
- Molecular Sensors and Ion Detection
- Chemical Synthesis and Analysis
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
Sorbonne Université
2009-2024
Institut de la Vision
2016-2024
Inserm
2009-2023
Centre National de la Recherche Scientifique
2017-2023
Howard Hughes Medical Institute
2015-2016
Janelia Research Campus
2016
Institut du Fer à Moulin
2009-2012
The mammalian retina conveys the vast majority of information about visual stimuli to two brain regions: dorsal lateral geniculate nucleus (dLGN) and superior colliculus (SC). degree which retinal ganglion cells (RGCs) send similar or distinct areas remains unclear despite important constraints that different patterns RGC input place on downstream processing. To resolve this ambiguity, we injected a glycoprotein-deficient rabies virus coding for expression fluorescent protein into dLGN SC;...
Abstract Remote and precisely controlled activation of the brain is a fundamental challenge in development brain–machine interfaces for neurological treatments. Low-frequency ultrasound stimulation can be used to modulate neuronal activity deep brain, especially after expressing ultrasound-sensitive proteins. But so far, no study has described an ultrasound-mediated strategy whose spatiotemporal resolution acoustic intensity are compatible with mandatory needs interfaces, particularly visual...
The K-Cl cotransporter KCC2 plays an essential role in neuronal chloride homeostasis, and thereby influences the efficacy polarity of GABA signaling. Although is expressed throughout somatodendritic membrane, it remarkably enriched dendritic spines, which host most glutamatergic synapses cortical neurons. has been shown to influence spine morphogenesis functional maturation developing neurons, but its function mature spines remains unknown. Here, we report that suppressing expression...
Abstract Vision restoration is an ideal medical application for optogenetics, because the eye provides direct optical access to retina stimulation. Optogenetic therapy could be used diseases involving photoreceptor degeneration, such as retinitis pigmentosa or age-related macular degeneration. We describe here selection, in non-human primates, of a specific optogenetic construct currently tested clinical trial. microbial opsin ChrimsonR, and showed that AAV2.7m8 vector had higher...
Heterozygous mutations in the CLCN2 gene encoding voltage-gated chloride channel CLC2 have been identified patients with idiopathic generalized epilepsy (IGE). Yet involvement of remains controversial. To investigate another independent sample, we screened 52 unrelated from IGE families and 23 Doose syndrome for CLCN2. No were found syndrome. In three families, two novel missense mutations, p.Arg235Gln p.Arg577Gln, which absent large ethnically-matched control populations, one p.Arg644Cys...
The brain receives information about the direction of object motion from several types retinal ganglion cells (RGCs). On-Off direction-selective (DS) RGCs respond preferentially to stimuli moving quickly in one four directions and provide a significant (but difficult quantify) fraction RGC input SC. On DS RGCs, comparison, slowly three are thought only target retinorecipient nuclei comprising accessory optic system, e.g., medial terminal nucleus (MTN). To determine SC-projecting that exhibit...
Dentate gyrus granule cells have been suggested to corelease GABA and glutamate both in juvenile animals under pathological conditions adults. Although mossy fiber terminals (MFTs) are known express glutamic acid decarboxylase (GAD) early postnatal development, the functional role of synthesis MFTs remains controversial, direct evidence for synaptic release from is missing. Here, using GAD67-GFP transgenic mice, we show that GAD67 expressed only a population immature animals. We demonstrate...
Over the last 15 years, optogenetics has changed fundamental research in neuroscience and is now reaching toward therapeutic applications. Vision restoration strategies using are at forefront of these new clinical opportunities. But applications to human patients suffering from retinal diseases leading blindness raise important concerns on long-term functional expression optogenes efficient signal transmission higher visual centers. Here, we demonstrate non-human primates continued...
Complete congenital stationary night blindness (cCSNB) due to mutations in TRPM1, GRM6, GPR179, NYX, or leucine-rich repeat immunoglobulin-like transmembrane domain 3 (LRIT3) is an incurable inherited retinal disorder characterized by ON-bipolar cell (ON-BC) defect. Since the disease non-degenerative and stable, treatment could theoretically be administrated at any time life, making it a promising target for gene therapy. Until now, adeno-associated virus (AAV)-mediated therapies lead...
Abstract Optogenetics has revolutionized neurosciences by allowing fine control of neuronal activity. An important aspect for this is assessing the activation and/or adjusting stimulation, which requires imaging entire volume optogenetically-induced ideal technique aim fUS imaging, allows one to generate brain-wide maps with submesoscopic spatial resolution. However, optical stimulation brain blue light might lead non-specific activations at high irradiances. optogenetic can be obtained...
Abstract Single-photon optogenetic stimulation is a crucial tool in neuroscience, enabling precise, cell-type-specific modulation of neuronal circuits. Miniaturization this technique the form fully implantable wide-field stimulator arrays enables interrogation cortical circuits long-term experiments and promises to enhance Brain-Machine Interfaces for restoring sensory motor functions. However, both basic science clinical applications, it essential that achieves precision needed selective...
Single-photon optogenetics enables precise, cell-type-specific modulation of neuronal circuits, making it a crucial tool in neuroscience. Its miniaturization the form fully implantable wide-field stimulator arrays long-term interrogation cortical circuits and bares promise for Brain-Machine Interfaces sensory motor function restoration. However, achieving selective activation functional representations poses challenge, as studies show that targeted optogenetic stimulation results activity...
Abstract Restoring vision using optogenetics is an ideal medical application because the eye offers a direct window to access and stimulate pathological area: retina. Optogenetic therapy could be applied diseases with photoreceptor degeneration such as retinitis pigmentosa. Here, we select specific optogenetic construct that now used in clinical trial assess opsin functional efficacy on non-human primate’s retinal ganglion cells (RGCs). We chose microbial ChrimsonR showed vector AAV2.7m8...
Abstract Optogenetic activation of neurons [1] have greatly contributed to our understanding how neural circuits operate, and holds huge promise in the field prosthetics, particularly sensory restoration. The discovery new channelrhodopsins, Chrimson — which is 45 nm more red-shifted than any previously discovered or engineered channelrhodopsin its mutant ChrimsonR with faster kinetics [2] made this technology available for medical applications. However, a detailed model that would be able...
Abstract Remote, precisely controlled activation of the brain is a fundamental challenge in development brain-machine interfaces providing feasible rehabilitation strategies for neurological disorders. Low-frequency ultrasound stimulation can be used to modulate neuronal activity deep 1–7 , but this approach lacks spatial resolution and cellular selectivity loads with high levels acoustic energy. The combination expression ultrasound-sensitive proteins (‘sonogenetic stimulation’) provide...
Optogenetic stimulation of the primary visual cortex (V1) is a promising therapy for sight restoration, but it remains unclear what total cerebral volume activated after surface stimulation. In this study, we expressed red-shifted opsin ChrimsonR in excitatory neurons within V1 rats, and used fine spatial resolution provided by functional ultrasound imaging (fUS) over whole depth brain to investigate response focal We observed optogenetic activation high proportion V1. Extracellular...