A5046662567- Toxin Mechanisms and Immunotoxins
- RNA modifications and cancer
- Cell death mechanisms and regulation
- Transgenic Plants and Applications
- Effects of Radiation Exposure
- Biomarkers in Disease Mechanisms
- Plant pathogens and resistance mechanisms
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Graphene and Nanomaterials Applications
- Gene expression and cancer classification
- Carbon and Quantum Dots Applications
- Plant Gene Expression Analysis
- Cancer Immunotherapy and Biomarkers
- Cancer, Stress, Anesthesia, and Immune Response
- Renal cell carcinoma treatment
- Nanoparticle-Based Drug Delivery
- Hepatitis B Virus Studies
- Natural product bioactivities and synthesis
- Hydrogen's biological and therapeutic effects
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Glycosylation and Glycoproteins Research
China Three Gorges University
2010-2024
Southwest Minzu University
2023
Zhongnan Hospital of Wuhan University
2018-2022
Wuhan University
2018-2022
Hubei Cancer Hospital
2019
Cancer Clinic
2019
10 B agents are critical components in the application of Boron Neutron Capture Therapy (BNCT) for tumor treatment. The development a multi-functional agent plays an important role the...
Objective: Increasing evidence suggested that dysregulated small nucleolar RNAs (snoRNAs) were involved in tumor development.The roles of snoRNA 71A (SNORA71A) the progression non-small cell lung cancer (NSCLC) remained unclear.Methods: Dataset GSE19188 from Gene Expression Omnibus (GEO) database was downloaded to detect expression levels SNORA71A NSCLC tissues.The biological significance explored by loss-of-function analysis both vitro and vivo.Results: overexpressed tissues compared with...
Epigenetic silencing of tumor suppressor genes is a well-established oncogenic process and the reactivation that have been silenced by promoter methylation an attractive molecular target for cancer therapy. In this study, we investigated demethylation activity trichosanthin (TCS, main bioactive component isolated from Chinese medicinal herb) its possible mechanism action in cervical cell lines. HeLa human adenocarcinoma CaSki squamous carcinoma cells were treated with various concentrations...
With the advancement of nanotechnology, various nanocomposites have been applied in diagnostics and treatment cancer.We synthetized FePt nanoparticles which were assembled on surface graphene oxide (GO).These novel FePt/GO nanosheets simultaneously act as a chemotherapy drug enhance radiosensitivity.In this study, transmission electron microscope, dynamic light scattering, X-ray photoelectron spectroscope Fourier transform infrared spectroscopy used to characterize morphology chemical...
Pneumonitis is a common adverse effect found in non-small cell lung cancer patients after radiotherapy or immune checkpoint inhibitor treatment. We investigated the effects of these two therapies, combined, tissue an orthotopic tumor-bearing mouse model. The mice received 8 Gy dose three times with without 200 µg anti-programmed death-1 (anti-PD-1) antibody intraperitoneal injection every days. Lung tissues were H&E stained to determine histological changes. serum levels cytokines, such as...
Radiation-induced lung injury (RILI) is one of the most common and fatal complications thoracic radiotherapy. Inflammatory cell infiltration, imbalance inflammatory cytokines, oxidative damage were reported to be involved during RILI pathogenesis, especially in early phase RILI. Nuclear factor-erythroid 2-related factor 2 (Nrf2) a key transcriptional regulator antioxidative cascades, regulates life span mice after administration irradiation. We investigated effects Nrf2 on inflammation using...
Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) has a specific antitumour activity against many malignant tumours.However, more than half of lung cancer cells are resistant to TRAIL-relevant drugs.Trichosanthin (TCS) is traditional Chinese medicine with strong inhibitive effects on various malignancies.Nevertheless, its function TRAIL resistance not been revealed in non-small cell (NSCLC).To examine the molecular mechanisms TCS-induced sensitivity, we administrated TCS...
The rabbit hemorrhagic disease virus 2 (RHDV2 or GI.2) is a highly contagious agent leading to lethal in rabbits. It frequently recombines with other Lagovirus genus, generating epidemical variants high pathogenicity. In this study, twenty-two liver samples tested positive for GI.2 VP60 gene, were collected farms from several geographical regions China. All specimens submitted RT-PCR detection, nucleotide sequencing and phylogenetic analysis. addition, suspected recombinants evaluated...
Mucin 3A (MUC3A) is highly expressed in non-small cell lung cancer (NSCLC), but its functions and effects on clinical outcomes are not well understood. Tissue microarray of 92 NSCLC samples indicated that high levels MUC3A were associated with poor prognosis, advanced staging, low differentiation. knockdown significantly suppressed proliferation induced G1/S accumulation via downregulating cycle checkpoints. also inhibited tumor growth vivo had synergistic radiation. increased...
Although recombinant human TNF‑related apoptosis‑inducing ligand (TRAIL) protein exhibits antitumor activity in a number of lung and liver cancer cells tumor‑bearing animals, TRAIL resistance has substantially restricted its clinical application. Pyrroline‑5‑carboxylate reductase 1 (PYCR1) is key enzyme the regulation proline synthesis. PYCR1 highly expressed various types malignant tumor, which it been implicated 5‑fluorouracil resistance. However, possible relationship between remains...
To observe the effects of high expression recombinant trichosanthin (rTCS) on cell proliferation and cycle human cervical cancer Caski cells.Eukaryotic plasmid pcDNA3.1(-)/6His-TCS was constracted stably transfected into cells. RT-PCR,Western-blot were used to select clones with rTCS high-expressing. Using pcDNA3.1(-)-transfected cells as control, MTT assay flowcytometry elucidate growth regulation in cells.The stable successfully established, which could inhibit (P<0.01) arrest G1 G2 phases...
Objectives. The most common subtype of renal cell carcinoma, clear carcinoma (ccRCC), has a high heterogeneity and aggressive nature. basement membrane (BM) is known to play vital role in tumor metastasis. BM-related genes remain untested ccRCC, however, terms their prognostic significance. Methods. were gleaned from the recent cutting-edge research. RNA-seq clinical data ccRCC obtained TCGA GEO databases, respectively. multigene signature was constructed using univariate Cox regression...
This research investigated the effect and mechanism of trichosanthin(TCS) in inducing autophagy apoptosis HeLa cells cervical cancer. Two-step chromatography was used to prepare TCS. MTT assay detect inhibition TCS on proliferation cells. The formation autophagic vesicles apoptotic bodies treated with observed under transmission electron microscopy, green fluorescent protein(GFP)-microtubule-associated protein 1 light chain beta 3(LC3B) fusion location laser confocal microscopy. expression...
Tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL) exhibits antitumor activity in various types of tumor cell and tumor‑bearing animals. However, acquired TRAIL resistance is a common issue that restricts its clinical application. Previous studies have revealed β‑catenin associated with melanoma colorectal tumors. In the present study, an acquired‑resistance non‑small‑cell lung cancer (NSCLC) line (H460‑TR) was established from parental TRAIL‑sensitive H460 cells using gradient...
Endoplasmic reticulum stress(ERS)is a kind of sub-cellular pathological process which result from calcium disorderliness and protein improper folding. And endoplasmic stress induced apoptosis is novel pathway different the mitochondria-initiated intrinsic death receptor-triggered extrinsic pathway. Trichosanthin (TCS), type I ribosome-inactivating protein, induces cell in various types including several tumor lines. However, mechanism remains largely uncharacterized. The aim present study to...
Abstract MUC3A is highly expressed in 40% of lung adenocarcinoma, but its functions and effects on clinical outcomes are not well understood. Tissue microarray 92 patients' tumor samples from Chinese National Human Genetic Resources Sharing Service Platform indicated that expression was associated with poor prognosis, advanced staging low differentiation. IP-MS co-IP results revealed interacted RELA activated the NFκB pathway via promoting phosphorylation interfering binding to IκB....
Abstract Background: MUC3A is highly expressed in lung adenocarcinoma, but its functions and effects on clinical outcomes are not well understood. Methods: Tissue microarray was used to analysis the relathionship between clinicalpathological charcristics of adenocarcinoma; Colony formation MTT array ware detect cell proliferation; WB applied investigate protein amount; Transwell evaluate invasion; IF observe location expression targeted proteins; IP use prove binding proteins....