A5103233209- Head and Neck Surgical Oncology
- Asthma and respiratory diseases
- Ion Transport and Channel Regulation
- Sinusitis and nasal conditions
- Oral and Maxillofacial Pathology
- Cystic Fibrosis Research Advances
- Bone Tumor Diagnosis and Treatments
- Tumors and Oncological Cases
- Head and Neck Cancer Studies
- Ocular Surface and Contact Lens
- Allergic Rhinitis and Sensitization
- Ion channel regulation and function
- Infectious Diseases and Mycology
- Corneal Surgery and Treatments
- Vascular Malformations and Hemangiomas
- Dermatology and Skin Diseases
- Ion Channels and Receptors
- Enzyme function and inhibition
- Salivary Gland Tumors Diagnosis and Treatment
- Respiratory and Cough-Related Research
- IgG4-Related and Inflammatory Diseases
- Neurofibromatosis and Schwannoma Cases
- Nasolacrimal Duct Obstruction Treatments
- Corneal surgery and disorders
- Polyamine Metabolism and Applications
Kyoto Prefectural University of Medicine
2016-2025
Ritsumeikan University
2023
Kyoto University
2004-2019
T cell phenotypes and kinetics are emerging as crucial factors associated with immunotherapeutic responses in a wide range of solid cancer types. However, challenges remain understanding the spatial temporal profiles cells differential due to difficulties single-cell analysis preserved tissue structures. Here, we provide an optimized 12-marker multiplex immunohistochemical (IHC) panel single-cell-based quantitative assessment identify distributions formalin-fixed paraffin-embedded sections....
Abstract Objectives Chronic low back pain (LBP) is known to cause various disorders compared with acute LBP. However, there was no study evaluating presenteeism due LBP divided into subcategories by the duration of Therefore, this aims investigate relationship between or chronic and in hospital nursing staff. Methods Overall, 1100 nurses filled a questionnaire on basic attributes, symptoms, depression work productivity. The subjects were three groups based period Work Limitation...
An association between bronchial asthma and sinusitis has long been suspected. Our aim is to study the clinical features of chronic associated with as two manifestations one airway disease.We conducted a prospective analysis outcome 88 patients, or without asthma, who underwent endoscopic sinus surgery (ESS) for sinusitis. Patients were divided into groups by presence absence evaluated. One surgeon performed ESS, same postoperative treatment was given both groups. The outcomes symptoms...
We investigated regulatory mechanisms of Cl(-) secretion playing an essential role in the maintenance surface fluid human airway epithelial Calu-3 cells. The present study reports that quercetin (a flavonoid) stimulated bumetanide-sensitive with reduction apical conductance, suggesting stimulates by activating entry step across basolateral membrane through Na(+)/K(+)/2Cl(-) cotransporter (NKCC1). To clarify mechanism stimulating NKCC1 quercetin, we verified involvement protein kinase (PK)A,...
Abstract Severe ocular surface diseases (OSDs) with severe dry eye can be devastating and are currently some of the most challenging disorders to treat. To investigate feasibility using an autologous tissue-engineered cultivated nasal mucosal epithelial cell sheet (CNMES) for reconstruction, we developed a novel technique culture cells expanded ex vivo from biopsy-derived human tissues. After protocol, CNMESs had 4–5 layers stratified, well-differentiated cells, successfully generated...
Nasal cavity and paranasal sinus have various functions. However, little information is available on ion transport in these upper airway epithelia. In the present study, we measured anion secretion channel activity to characterize epithelial cells prepared from human mucosa (PSM) nasal polyp (NP). To estimate activity, short-circuit current (Isc) transepithelial conductance (Gt) sensitive NPPB (a Cl(-) blocker). The NPPB-sensitive Isc PSM was larger than that NP, correlating Gt (Cl(-)...
An application of CO2/HCO3--free solution (Zero-CO2) did not increase intracellular pH (pHi) in ciliated human nasal epithelial cells (c-hNECs), leading to no the beating frequency (CBF) or bend distance (CBD, an index amplitude). This study demonstrated that pHi c-hNECs expressing carbonic anhydrase IV (CAIV) is high (7.64), while bronchial (c-hBECs) CAIV low (7.10). Upon applying Zero-CO2, extremely level caused pHi, CBF and CBD decrease, a them increase. The was generated by rate HCO3-...
The effects of the isoflavone daidzein on ciliary beat distance (CBD, which is a parameter assessing amplitude beating) and frequency (CBF) were examined in ciliated human nasal epithelial cells (cHNECs) primary culture. Daidzein decreased [Cl−]i enhanced CBD cHNECs. increase that was stimulated by mimicked Cl−-free NO3− solution bumetanide (an inhibitor Na+/K+/2Cl− cotransport), both [Cl−]i. Moreover, inhibited 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, Cl− channel blocker),...
Invasive fungal rhinosinusitis (FRS) is a rare but intractable infectious disease of the sinonasal region with destructive direct infiltration into surrounding tissues, such as bone, orbit and brain, potential dissemination to systemic organs. Symptomatic assessments imaging are frequently not sufficiently diagnostic, histopathological examination essential for definite diagnosis FRS. We herein report case chronic invasive FRS (CIFRS) in 58-year-old Japanese male end-stage diabetic...
Objective Carbocisteine (CCis), a mucoactive agent, is used to improve the symptoms of sinonasal diseases. However, effect CCis on nasal ciliary beating remains uncertain. We examined effects beat distance (CBD, an index amplitude), and frequency (CBF) in ciliated human epithelial cells (cHNECs) primary culture. Methods The cHNECs were prepared from tissue resected patients required surgery for chronic sinusitis (CS) or allergic rhinitis (AR). CBD CBF measured using videomicroscopy equipped...
We evaluated the efficacy and toxicity of reirradiation nasal cavity or paranasal sinus tumors. collected analyzed multi-institutional data cases. Seventy-eight patients with tumors underwent reirradiation. The median survival time was 20 months a medial follow-up 10.7 months. 2-year local control overall rates were 43% 44%, respectively. Tumor volume (≤25 cm3), duration between previous radiotherapy (≤12 months), histology (squamous cell carcinoma), male sex, lymph node involvement...
An application of CO2/HCO3−-free solution (Zero-CO2) did not increase intracellular pH (pHi) in ciliated human nasal epithelial cells (c-hNECs), leading to no frequency (CBF) or amplitude (CBA) the ciliary beating. This study demonstrated that pHi c-hNECs expressing carbonic anhydrase IV (CAIV) is high (7.64), while bronchial (c-hBECs) CAIV low (7.10). extremely caused pHi, CBF and CBA decrease upon Zero-CO2 application, a c-hBECs them increase. was generated by rate HCO3− influx via...
We report a 50-year old man with chronic sinusitis frontal sinus cyst showing intracranial development. He complained of swelling in the medial angle right eyelid. Preoperative CT and MRI demonstrated ethmoid-maxillary sinusitis. The compressed lobe cerebrum, but no symptom was accompanied by impairment. Under general anesthesia, were accessed intranasal ESS. gently fenestrated wall microdebrider drained cystic component. patient has been event free recurrence for 15 months postoperatively.
When animals are infected with helminthic parasites, resistant hosts mount type II helper T (Th2) immune responses to expel worms. Recent studies have clearly shown that epithelial cell-derived cytokines contribute the induction of Th2 responses. Here we demonstrate role endogenous thymic stromal lymphopoietin (TSLP) for protection against Strongyloides venezuelensis (S. venezuelensis) infection, utilizing TSLP receptor-deficient Crlf2−/− mice. The number eggs per gram feces (EPG) and worm...
We report two cases of a foreign body in the hypopharynx and esophagus, removed by an external incision neck. The first case was 71-year-old male who complained sore throat after eating fish. No detected pharyngolaryngoscopy, but CT scan revealed fish bone his abscess formation detected. following transcervical drainage abscess. second 62-year-old female with extrapharyngeal due to swallowing bone. A demonstrated her space. successfully lateral neck incision.
We present a case of primary systemic amyloidosis diagnosed by endoscopic sinus surgery. A 75-year-old woman had blurred vision in her left eye; computed tomography and magnetic resonance imaging showed shadows the bilateral paranasal sinuses. Endoscopic surgery was performed, histopathology. She previously been with stomach, therefore, she amyloidosis. workup for additional amyloid deposits revealed no evidence other diseases. The patient remained under follow-up without further treatment,...
2005年4月から2012年3月に京都府立医科大学附属病院耳鼻咽喉科で治療した上顎洞扁平上皮癌T3，T4の19症例について，その治療成績や再発形式を後ろ向きに調べ，動注化学療法併用放射線治療後の切除標本における腫瘍細胞の残存率と予後の関係について検討した。疾患特異的5年生存率が68.4.%，特にT3，T4aは75%程度と良好であった。再発は9症例で，原発巣再発は2例，遺残が2例であった。動注併用放射線治療後の摘出標本で腫瘍細胞が少ないほど，局所制御率が向上し，生存率が良くなる傾向であった。動注併用放射線治療と手術を組み合わせた本治療は，優れた局所制御率，生存率を有すると考えられた。
軟骨肉腫のうち頭頸部領域に発生するものは全体の1~12%であり,鼻中隔に発生するものは稀である。軟骨肉腫は放射線療法や化学療法の感受性が一般に低いとされ,外科的切除が基本となる。我々は鼻中隔に発生した軟骨肉腫の1例を経験した。症例は56歳男性,主訴は左鼻閉であった。CTで鼻中隔後方に最大径36.5mmの内部斑状の石灰化を伴う腫瘤を認めた。前医での生検で軟骨腫瘍の疑いにて当科紹介となり,当院で再検討し軟骨肉腫の診断となった。本症例は低悪性度の軟骨肉腫が疑われたこと,術前の画像診断で頭蓋底への進展を認めなかったことより,内視鏡操作で安全域をつけて切除可能と判断したため術前に腫瘍の栄養血管塞栓術を行い,術中迅速病理診断を利用することにより分割切除で内視鏡下に腫瘍を摘出した。本症例では現在術後2年経過しているが,再発や転移は認めていない。