A5101986851- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Magnetic Properties of Alloys
- Carbohydrate Chemistry and Synthesis
- Power Systems and Renewable Energy
- Magnetic and transport properties of perovskites and related materials
- Advanced Condensed Matter Physics
- Microgrid Control and Optimization
- Epigenetics and DNA Methylation
- Rare-earth and actinide compounds
- Asymmetric Hydrogenation and Catalysis
- Ubiquitin and proteasome pathways
- Power Quality and Harmonics
- Cancer, Hypoxia, and Metabolism
- High-Voltage Power Transmission Systems
- Neuroblastoma Research and Treatments
- Asymmetric Synthesis and Catalysis
- Metallic Glasses and Amorphous Alloys
- Advanced DC-DC Converters
- Supramolecular Self-Assembly in Materials
- Autophagy in Disease and Therapy
- Glycosylation and Glycoproteins Research
- Geomagnetism and Paleomagnetism Studies
- Biochemical and Structural Characterization
- Advanced Glycation End Products research
Shanghai Jiao Tong University
2020-2024
Shanghai Children's Medical Center
2020-2024
Qingdao University
2024
Affiliated Hospital of Qingdao University
2024
Tianjin Central Hospital of Gynecology Obstetrics
2021-2022
Nankai University
2022
Naval University of Engineering
2015-2021
Air Force Engineering University
2021
Beijing Friendship Hospital
2020
Capital Medical University
2020
An efficient method has been developed for the salicylaldehyde ester-mediated ligation of unprotected peptides at serine (Ser) or threonine (Thr) residues. The utility this peptide approach demonstrated through convergent syntheses two therapeutic peptides––ovine-corticoliberin and Forteo––and human erythrocyte acylphosphatase protein (∼11 kDa). requisite ester precursor is prepared in an epimerization-free manner via Fmoc–solid-phase synthesis.
A serine/threonine-based chemoselective ligation method is described. It uses an O-salicylaldehyde ester at the C-terminus, reacting with N-terminal serine or threonine to realize peptide ligations. The utility of enables rapid coupling reaction and production N,O-benzylidene acetal intermediate, which readily converted into natural peptidic linkages (Xaa-Ser/Thr) site.
A total synthesis of daptomycin, the first natural product antibiotic launched in a generation, was achieved. This convergent relies on an efficient macrocyclization via serine ligation to assemble 31-membered cyclic depsipeptide. The difficult esterification by nonproteinogenic amino acid kynurenine accomplished threonine residue suitably protected Trp ester, followed ozonolysis. provides foundation and framework prepare varied analogues daptomycin establish its structure-activity profile.
Drug efflux represents an important protection mechanism in bacteria to withstand antibiotics and environmental toxic substances. Efflux genes constitute 6-18% of all transporters bacterial genomes, yet the expression functions only a handful them have been studied. Among 20 encoded Escherichia coli K-12 genome, AcrAB-TolC system is constitutively expressed. The expression, activities, physiological remaining are poorly understood. In this study we identified dramatic up-regulation...
E2 conjugating enzymes are the key catalytic actors in transfer of ubiquitin, SUMO, and other ubiquitin-like modifiers to their substrate proteins. Their high rates promiscuous binding complicate studies interactions partners. To access specific, covalently linked conjugates SUMO enzyme Ubc9, we prepared synthetic variants bearing site-specific non-native modifications including following: (1) replacement Cys93 2,3-diaminopropionic acid form amide-linked stable E2–SUMO conjugate, which is...
Syntheses of MUC1 glycopeptides (40-mer and 80-mer) are described. The convergent synthesis was achieved by native serine ligation using side-chain unprotected glycopeptide segments.
WDR5 specifically recognizes histone H3Q5ser to promote gene transcription and neuroblastoma cell proliferation.
Serine/Threonine ligation (STL) has emerged as an alternative tool for protein chemical synthesis, bioconjugations well macrocyclization of peptides various sizes. Owning to the high abundance Ser/Thr residues in natural and proteins, STL is expected find a wide range applications biology research. Herein, we have fully investigated compatibility strategy X-Ser/Thr sites, where X any 20 naturally occurring amino acids. Our studies shown that 17 acids are suitable ligation, while Asp, Glu,...
The synthesis of human growth hormone-releasing hormone (hGH-RH), by the chemoselective serine/threonine ligations (STLs) three unprotected peptide fragments, is reported. To allow for multiple-fragment ligation, we chose Msz (p-(methylsulfinyl)benzyloxycarbonyl) group, which compatible with preparation salicylaldehyde esters via Fmoc-SPPS and readily removed reductive acidolysis, to protect serine threonine residue at N-terminus.
The grain boundary diffusion treatment using the low melting point Pr-Cu eutectic alloy was applied to enhance coercivity of anisotropic die-upset Nd-Fe-B powders. It found that room temperature powders increases from 13 kOe 24 kOe. thickness layer increased and Fe Co content in phases decreased. modified layers may thus increase domain wall nucleation field, leading an enhancement coercivity. prevents loss continuity Nd-rich phase retains crystal texture initial grains.
Although periconception vaccination is important to maternal and neonatal health, little known about the COVID-19 vaccine hesitancy among infertile couples seeking fertility treatment. Thus, we conducted this survey patients in a reproductive medicine center, between September 2021 December 2021, estimate prevalence of its influencing factors. Information was collected through face-to-face interviews volunteers. Among 987 included interviewees, 17.33% reported primary vaccination, 25.63%...
Neuroblastoma (NB) is the most common extracranial solid tumor in children. Despite treatment advances, survival rates of high-risk NB patients remain low. This highlights urgent need for a deeper understanding molecular mechanisms driving progression to support development new therapeutic strategies. In this study, we demonstrated that reduced levels DNAJC12, protein involved metabolic regulation, are associated with poor prognosis patients. Our data indicate low DNAJC12 expression...
Three genes from a shotgun genomic library of Flavobacterium columnare virulent strain ALG-00-530 were identified, characterized, and selected for differential expression analysis based on sequence similarity to putative virulence related species.These were: glycosyltransferase (gtf), nitric oxide reductase (norB) thioredoxin (trx).A collection 30 F. strains, including strains genomovars I II, tested the presence these genes.Distribution patterns gtf, norB, trx across species not...
Nd2Fe14B/α-Fe nanocomposite magnets are prepared through electron beam exposure with a greatly reduced annealing time of 0.1 s. This is by far the most effective approach due to effect an extremely high heating rate featuring rapid thermal process. The impact that process has on crystallization expounded introduction Landau model and Langevin dynamical simulations. change sequence from α-Fe phase preceding Nd2Fe14B under conventional conditions, synergetic conditions investigated. Synergetic...
In many aspects, harmonics will cause a series of problems such as electromagnetic interference (EMI), mechanical vibration and harsh noise. Especially when the amplitude is high, more serious problem arise. For these reasons, this paper has made an analysis theory random carrier modulation presented practical technique which applied to pulse-width (PWM). Random (RCM) unique superiority in expanding distribution harmonic spectrum, great value dispersing balancing energy whole frequency...
In this paper, we present a novel hybrid driving method to improve pitting issue caused by inhomogeneous efficiency of LED device in low luminous situation. By applying PWM (Pulse‐Width‐Modulation) gray scale region and PAM (Pulse‐Amplitude‐Modulation) middle high region, achieved verifying the PHM (Pulse‐Hybrid‐Modulation) mini display platform.
<title>Abstract</title> Dopaminylation, the covalent attachment of dopamine to side chain glutamine (Gln, Q) in proteins, has been identified as a class posttranslational modification. Due limited number substrates, functions and underlying molecular mechanisms dopaminylation are not fully characterized. Utilizing an alkyne-functionalized probe, we have developed method selectively enrich dopaminylated proteins whole-cell context. We 4,133 potentially modified with validated modification...
Abstract According to the linear quadratic optimal control principle, idea of tracking is applied three-phase inverter system, which can effectively improve output characteristics system. Based on establishment mathematical model, nonlinear load and mutation are regarded as uncertain factors affecting performance inverter, parameter optimization design state feedback input feedforward carried out based method. The simulation results show that LQR better adapt adverse effects than traditional...
It is crucial to characterize early Maillard reaction products and the important compounds formed in stage of reactionas Amadori rearrangement are most modifications food science.We report here that using electrospray ionizationmass spectrometry (ESI-MS) directly fragmentation behaviour a model system six selected amino acids (arginine, asparagine, glutamine, histidine, lysine tryptophan) their N-terminal acetylated forms with two reducing disaccharides, lactose maltose.The was illustrated...