A5052153025- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Antimicrobial Resistance in Staphylococcus
- Cancer-related molecular mechanisms research
- Ion Transport and Channel Regulation
- Enzyme Structure and Function
- Atherosclerosis and Cardiovascular Diseases
- interferon and immune responses
- Sarcoma Diagnosis and Treatment
- Bacillus and Francisella bacterial research
- Radioactive element chemistry and processing
- Cell death mechanisms and regulation
- PARP inhibition in cancer therapy
- RNA Interference and Gene Delivery
- Trypanosoma species research and implications
- Cancer-related gene regulation
- Polyamine Metabolism and Applications
- Bacterial Genetics and Biotechnology
- Sirtuins and Resveratrol in Medicine
- Trace Elements in Health
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Membrane-based Ion Separation Techniques
- Protein Structure and Dynamics
Chinese Academy of Sciences
2017-2024
Shenzhen Institutes of Advanced Technology
2024
University of Science and Technology of China
2017-2022
Shanghai Children's Medical Center
2020-2022
Center for Excellence in Molecular Cell Science
2022
Shanghai Jiao Tong University
2020-2022
Hefei National Center for Physical Sciences at Nanoscale
2021
Hefei University
2021
Hefei Institutes of Physical Science
2017-2018
Tianjin Medical University
2018
Tetrathiomolybdate (TM) is used in the clinic for treatment of Wilson's disease by targeting cellular copper efflux protein ATP7B (WLN). Interestingly, both TM and WLN are associated with efficacy cisplatin, a widely anticancer drug. Herein, we show that induces dimerization metal-binding domain (WLN4) through unique sulfur-bridged Mo2S6O2 cluster. expels ions from Cu-WLN4 forms copper-free dimer. The binding Mo to cysteine residues WLN4 inhibits platination protein. Reaction multi-domain...
WDR5 specifically recognizes histone H3Q5ser to promote gene transcription and neuroblastoma cell proliferation.
Abstract Pentamidine and melarsoprol are primary drugs used to treat the lethal human sleeping sickness caused by parasite Trypanosoma brucei . Cross-resistance these two has recently been linked aquaglyceroporin 2 of trypanosome (TbAQP2). TbAQP2 is first member aquaporin family described as capable drug transport; however, underlying mechanism remains unclear. Here, we present cryo-electron microscopy structures bound pentamidine or melarsoprol. Our structural studies, together with...
Abstract Epigenetic modifiers have emerged as critical factors governing the biology of different cancers. Herein we show that FBXL10 (also called KDM2B or JHDM1B), an important member Polycomb repressive complexes, is overexpressed in human diffuse large B-cell lymphoma (DLBCL) tissues and derived cell lines. Knocking down by specific short hairpin RNAs DLBCL cells inhibits proliferation induces apoptosis vitro . Moreover, depletion abrogates tumor growth mouse xenograft models. Through...
Histone modification is a ubiquitous regulatory mechanism involved in variety of biological processes, including gene expression, DNA damage repair, cell differentiation, and ontogenesis. Succinylation sites on histones have been identified may functional consequences. Here, we demonstrate that human sirtuin 5 (Sirt5) catalyzes the sequence-selective desuccinylation numerous histone succinyl sites. Structural studies Sirt5 complex with four peptides indicate an essential role for conserved...
Abstract Staphylococcal aureus ( S. ) infection can lead to a wide range of diseases such as sepsis and pneumonia. superantigen-like (SSL) proteins, expressed by all known strains, are shown be involved in immune evasion during infection. Here, we show that SSL10, an SSL family protein, exhibits potent cytotoxicity against human cells (HEK293T HUVEC) inducing necroptosis upon binding its receptor TNFR1 on the cell membrane. After binding, two distinct signaling pathways activated downstream...
Background: Rhabdomyosarcoma (RMS) is one of the most common types soft-tissue sarcomas in children, and it exhibits a low 5-years survival rate. The outcome has shown no significant improvements past 30 years miRNA profiling RMS might therefore provide novel insight into uncovering new molecular targets for therapy. Methods: We analyzed RNA sequencing data from patients TARGET database to reveal potential miRNA-mRNA axes validated them patients' samples. After antagomirs were used silence...
Abstract Serotonylation of histone H3Q5 (H3Q5ser) is a recently identified posttranslational modification histones that apparently acts as permissive marker for gene activation in synergy with H3K4me3 during neuronal cell differentiation. However, any proteins which specifically recognize H3Q5ser remain unknown. Here, we discovered WDR5 interacts the N-terminal tail H3 and functions ‘reader’ H3Q5ser. Crystal structures complex H3K4me3Q5ser peptides revealed serotonyl group accommodated...
Abstract Staphylococcal aureus ( S. ) infection can lead to a wide range of diseases such as sepsis and pneumonia. superantigen like (SSL) proteins, specifically expressed by , are shown be involved in immune evasion during infection. Here, we show that SSL10, an SSL family protein, exhibits potent cytotoxicity against human cells (HEK 293T HUVEC) inducing necroptosis upon binding its receptor TNFR1 on the cell membrane. After binding, two distinct signaling pathways activated downstream...
Background: Henoch-Schönlein Purpura (HSP) is one of the most common systemic small vasculitis in children. At present, it believed that genetic factors play an important role etiology and pathogenesis HSP.Methods: In order to find susceptible genes associated with purpura (HSP), whole exome sequencing (WES) was carried out 4 Chinese families HSP. Then, ULBP5 sequenced by Polymerase Chain Reaction (PCR) 123 unrelated patients HSP group (UHSPG), 115 connective tissue disease (CTDG, not...