A5021502041- Plant Molecular Biology Research
- Enzyme Production and Characterization
- DNA Repair Mechanisms
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- DNA and Nucleic Acid Chemistry
- Phytochemical compounds biological activities
- Histone Deacetylase Inhibitors Research
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Neonatal Health and Biochemistry
- Biochemical and Molecular Research
- Legume Nitrogen Fixing Symbiosis
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Heat shock proteins research
- Plant nutrient uptake and metabolism
- Peptidase Inhibition and Analysis
- Cell death mechanisms and regulation
- Parasite Biology and Host Interactions
- Microbial Metabolites in Food Biotechnology
- Plant Pathogenic Bacteria Studies
- Cancer therapeutics and mechanisms
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
Zhejiang Ocean University
2025
National Taiwan University
2014-2024
Academia Sinica
1999-2023
National Taiwan Normal University
2021
Central China Normal University
2017
Institute of Plant and Microbial Biology, Academia Sinica
2011
Institute of Molecular Biology, Academia Sinica
2000-2007
National Yang Ming Chiao Tung University
2004
National Defense Medical Center
1999-2000
National Taipei University of Technology
2000
Mcl-1 is one Bcl-2 family member that plays a pivotal role in animal development. The extremely labile nature of the protein itself and fact level critical determinant various cell survival pathways suggest cellular processes regulate stability are as important those synthesis. Although transcriptional stimulation synthesis response to stimuli has been well documented, regulation hardly explored. In this study, we identified translationally controlled tumor (TCTP) was factor interacted with...
Histone deacetylases (HDACs) play important roles in regulating gene expression. In yeast and animals, HDACs act as components of multiprotein complexes that modulate transcription during various biological processes. However, little is known about the interacting proteins plant HDACs. To identify HDAC proteins, we developed an optimized workflow using immunopurification coupled to mass spectrometry-based proteomics Arabidopsis thaliana We found histone deacetylase HDA6 can interact with...
Whole-cell biosensors are useful for monitoring heavy metal toxicity in public health and ecosystems, but their development has been hindered by intrinsic trade-offs between sensitivity specificity. Here, we demonstrated an effective engineering solution building a sensitive, specific, high-response biosensor carcinogenic cadmium ions. We genetically programmed the transport system of Escherichia coli to enrich intracellular ions deprive interfering species. then selected 16 cadmium-sensing...
Against the backdrop of rapid development modern logistics, chemical logistics industry is one important sub-sectors and also developing rapidly. The country a major producer user dangerous goods such as hazardous chemicals, explosives, radioactive materials, in road transportation goods. In recent years, enterprises governments have gradually upgraded their awareness safety transportation, paid more attention to efficiency transportation. This paper selects Yonggang Haian Logistics Co.,...
We designed and synthesized quinazolin-2,4-dione-based hydroxamic acids to serve as selective competitive inhibitors of histone deacetylase-6 (HDAC6). The most potent compound, 3d (IC50, 4 nM, HDAC6; IC50 > 10 μM, HDAC1), substantially increased acetylation α-tubulin instead histones in the lung cancer cell line, LL2. Paclitaxel combination with had a synergistic anticancer effect on reduction programmed death-ligand 1 expression LL/2 cells. When given orally, was mainly found locate liver...
This article presents the design of a sequence-based predictor named ProteDNA for identifying sequence-specific binding residues in transcription factor (TF). Concerning protein–DNA interactions, there are two types mechanisms involved, namely and nonspecific binding. Sequence-specific bindings occur between protein sidechains nucleotide bases correspond to recognition genes. Therefore, essential correct gene regulation. In this respect, is distinctive since it has been designed identify...
Abstract The membrane protein AtTLP18.3 of Arabidopsis (Arabidopsis thaliana) contains a domain unknown function, DUF477; it forms polysome with photosynthetic apparatuses in the thylakoid lumen. To explore molecular function AtTLP18.3, we resolved its crystal structures residues 83 to 260, DUF477 only, and performed series biochemical analyses discover function. gene expression followed circadian rhythm. X-ray crystallography revealed folding as three-layer sandwich three α-helices upper...
Tarocystatin (CeCPI) from taro (Colocasia esculenta cv. Kaohsiung no. 1), a group-2 phytocystatin, shares conserved N-terminal cystatin domain (NtD) with other phytocystatins but contains C-terminal cystatin-like extension (CtE). The structure of the tarocystatin-papain complex and interaction between NtD CtE in tarocystatin have not been determined. We resolved crystal phytocystatin-papain at resolution 2.03 Å. Surprisingly, NtD-papain stoichiometry 1:1 could be built, no observed. Only two...
α-L-Rhamnosidase is a glycoside hydrolase capable of removing naringin from citrus juice. However, α-L-rhamnosidases always have broad substrate spectra, causing negative effects on In this study, α-L-rhamnosidase-expressing fungal strain, JMU-TS529, was identified, and its α-L-rhamnosidase characterized. As result, JMU-TS529 identified as Aspergillus tubingensis via morphological molecular characteristics. The predicted protein sequence shared an amino acid identity less than 30% with...
The Arabidopsis H3K9 methyltransferases KRYPTONITE/SUPPRESSOR OF VARIEGATION 3-9 HOMOLOG 4 (KYP/SUVH4), SUVH5 and SUVH6 are redundantly involved in silencing of transposable elements (TEs). Our recent study indicated that KYP/SUVH5/6 can directly interact with the histone deacetylase HDA6 to synergistically regulate TE expression. However, function plant development is still unclear. transcriptional factors ASYMMETRIC LEAVES1 (AS1) AS2 form a transcription complex, which leaf by repressing...
Tarocystatin from Colocasia esculenta, a group-2 phytocystatin, is defense protein against phytopathogenic nematodes and fungi. It composed of highly conserved N-terminal region, which homological to group-1 cystatin, repetitive peptide at the C-terminus. The purified recombinant proteins tarocystatin, such as full-length (FL), N-terminus (Nt) C-terminus (Ct) peptides, were produced their inhibitory activities papain well antifungal effects investigated. Kinetic analysis revealed that FL...
Abstract The phytohormone ethylene is widely involved in many developmental processes and a crucial regulator of defense responses against biotic abiotic stresses plants. Ethylene-responsive element binding protein, member the APETALA2/ethylene response factor (AP2/ERF) superfamily, transcription that regulates stress-responsive genes by recognizing specific cis -acting target DNA. A previous study showed only NMR structure AP2/ERF domain AtERF100 complex with GCC box DNA motif. In this...
Catalytic efficiency and thermostability are the two most important characteristics of enzymes. However, it is always tough to improve both catalytic enzymes simultaneously. In present study, a computational strategy with double-screening steps was proposed simultaneously catalysis enzymes; fungal α-l-rhamnosidase used validate strategy. As result, by molecular docking sequence alignment analysis within binding pocket, seven mutant candidates were predicted better efficiency. By energy...
WRKY transcription factors (TFs), which make up one of the largest families TFs in plant kingdom, are key players modulating gene expression relating to embryogenesis, senescence, pathogen resistance, and abiotic stress responses. However, phylogeny grouping how their binding ability is affected by flanking regions W-box sequences remain unclear. In this study, we reconstructed across kingdom characterized DNA-binding profile Arabidopsis thaliana (WRKY54) based on its recognition sequence....
The nuclease domain of ColE7 (N-ColE7) contains an H-N-H motif that folds in a beta alpha-metal topology. Here we report the crystal structures Zn2+-bound N-ColE7 (H545E mutant) complex with 12-bp duplex DNA and Ni2+-bound inhibitor Im7 at resolution 2.5 A 2.0 A, respectively. Metal-dependent cleavage assays showed cleaves double-stranded single metal ion cofactor, Ni2+, Mg2+, Mn2+, Zn2+. purified from Escherichia coli endogenous zinc was not replaced by Mg2+ concentrations <25 mM,...
Many proteins are induced in the plant defense response to biotic stress or mechanical wounding.One group is lectins.Ipomoelin (IPO) one of wound-inducible sweet potato (Ipomoea batatas cv.Tainung 57) and a Jacalinrelated lectin (JRL).In this study, we resolved crystal structures IPO its apo form complex with carbohydrates such as methyl a-D-mannopyranoside (Me-Man), a-D-glucopyranoside (Me-Glc), a-D-galactopyranoside (Me-Gal) different space groups.The packing diagrams indicated that might...
HDAC6 receives great attention because of its therapeutic potential for the treatment various diseases.
Reactive oxygen species (ROS) play a crucial role in regulating numerous functions organisms. Among the key regulators of ROS production are NADPH oxidases, primarily referred to as respiratory burst oxidase homologues (RBOHs). However, our understanding whether and how pathogens directly target RBOHs has been limited. In this study, we revealed that effector protein RipBJ, originating from phytopathogenic bacterium Ralstonia solanacearum, was present low- medium-virulence strains but absent...