A5022534800- Alzheimer's disease research and treatments
- Advanced Proteomics Techniques and Applications
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Neuropeptides and Animal Physiology
- Metabolomics and Mass Spectrometry Studies
- S100 Proteins and Annexins
- COVID-19 epidemiological studies
- Brain Metastases and Treatment
- Cellular transport and secretion
- Anesthesia and Neurotoxicity Research
- Biotin and Related Studies
- RNA Research and Splicing
- Tryptophan and brain disorders
- Anesthesia and Sedative Agents
- Ferroptosis and cancer prognosis
- Intensive Care Unit Cognitive Disorders
- Biochemical and Structural Characterization
- Protein Hydrolysis and Bioactive Peptides
- Histone Deacetylase Inhibitors Research
- Neurological Disorders and Treatments
- Neuroscience and Neuropharmacology Research
Yale University
2021-2024
Massachusetts General Hospital
2019-2022
Harvard University
2019-2022
Brigham and Women's Hospital
2019
Hebrew SeniorLife
2019
Hadassah Medical Center
2019
Beth Israel Deaconess Medical Center
2019
Alzheimer's disease (AD) is defined by the presence of abundant amyloid-β (Aβ) and tau neuropathology. While this neuropathology necessary for AD diagnosis, it not sufficient causing cognitive impairment. Up to one third community dwelling older adults harbor intermediate high levels at death yet demonstrate no significant Conversely, there are individuals who exhibit dementia with gross explanatory In prior studies, synapse loss correlated To understand how synaptic composition changes in...
COVID-19 has resulted in significant morbidity and mortality worldwide. Lateral flow assays can detect anti-Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) antibodies to monitor transmission. However, standardized evaluation of their accuracy tools aid interpreting results are needed.We evaluated 20 IgG IgM selected from available tests April 2020. We the assays' performance using 56 pre-pandemic negative SARS-CoV-2-positive plasma samples, collected 10-40 days after symptom...
The Role of Inflammation after Surgery for Elders study correlates novel inflammatory markers measured in blood, cerebrospinal fluid (CSF) assays, and [11C]-PBR28 positron-emission tomography imaging. This involved a prospective cohort design with patients who underwent elective hip knee arthroplasty under spinal anesthesia. Sixty-five adults participated their family members. Inflammatory biomarker assays were preoperatively on day 1 postoperatively at one month. On average, participants 75...
Proteomic characterization of human brain tissue is increasingly utilized to identify potential novel biomarkers and drug targets for a variety neurological diseases. In whole-tissue studies, results may be driven by changes in the proportion largest most abundant organelles or cell-type composition. Spatial proteomics approaches enhance our knowledge disease mechanisms changing signalling pathways at subcellular level taking into account importance cellular localization, which critically...
Abstract Leptomeningeal disease (LMD) is a rare form of central nervous system (CNS) metastasis wherein tumor cells invade the cerebrospinal fluid (CSF) filled space that surrounds brain and spinal cord. For patients with LMD, prognosis extremely poor even aggressive treatment. The mechanisms progression to LMD adaptations make survive in this metabolically sparse microenvironment are poorly understood. As 60% have concurrent or prior parenchymal metastases, our laboratory examined...
Alzheimer's disease (AD) is a complex neurodegenerative defined by the presence of amyloid-beta (Aβ) plaques and tau neurofibrillary tangles, driven dysproteostatis, inflammation, metabolic dysfunction, oxidative injury, eventually leading to synapse loss cell death. Synapse correlates with cognitive impairment may occur independently extent AD pathology. To understand how synaptic composition changed in relation neuropathology cognition, highly sensitive multiplexed liquid chromatography...
Abstract Background COVID-19 has resulted in significant morbidity and mortality worldwide. Lateral flow assays can detect anti-Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) antibodies to monitor transmission. However, standardized evaluation of their accuracy tools aid interpreting results are needed. Methods We evaluated 20 IgG IgM selected from available tests April 2020. the assays’ performance using 56 pre-pandemic negative SARS-CoV-2-positive plasma samples, collected...
Abstract Background Chromogranins and secretogranins are neurotransmitter‐like molecules (neuropeptides) that processed into functional peptides (proteoforms) by proteases. Full‐length chromogranins their peptide proteoforms involved in diverse neuronal synaptic functions, but specific processing pathways contribution to dementia remain largely unknown. This project aims investigate chromogranin secretogranin proteolytic validate if may be useful cerebrospinal fluid (CSF) biomarkers of...
Alzheimer's Disease (AD) is a complex disorder with disturbances in many cellular pathways affecting multiple organelles. While there are methods that can reliably enrich for organelles fresh brain tissue, these have shown more modest success frozen human neural tissue variable post mortem interval. The aim of this pilot experiment was to develop reliable method the fractionation enable subcellular proteomic analysis AD brain. We used ultracentrifugation-based and liquid chromatography mass...
Alzheimer's disease (AD) is a complex driven by cycles of protein misfolding, inflammation, and neurovascular metabolic dysfunction. Although tau amyloid-β levels in cerebrospinal fluid (CSF) are strong indicators AD pathology, they inadequate for measuring progression or drug response. To enable earlier diagnosis expand opportunities intervention, it critical to develop more sensitive diverse biomarkers AD. Quantifying proteins pathways that drive may provide better susceptibility,...
Current diagnosis of Alzheimer's disease (AD) relies primarily on cognitive assessments supported by imaging and a small number cerebrospinal fluid (CSF) biomarkers. These limited biomarkers fail to capture the complexity may be inadequate predictors treatment response. Novel are required better diagnose assess AD activity treatments efficacy. Given its intimate connection with brain's interstitial fluid, CSF is an ideal biofluid for biomarker discovery. In order identify possible presence,...
Alzheimer's Disease (AD) is a complex neurodegenerative disease, driven by intersecting cycles of insoluble protein deposition, inflammation, oxidative injury and other abnormalities resulting in synapse loss cell death. Although amyloid (Aβ) tau pathologies are necessary for AD diagnosis, they not sufficient explaining disease expression, it uncommon individuals to harbor widespread plaque tangle pathology yet retain intact cognition. As thought be the best correlate cognitive impairment...
Neuropeptides are neurotransmitter-like molecules with multiple roles in neuronal activity that processed into functional peptides (aka proteoforms) by prohormone convertases and other currently unidentified proteases. Both neuropeptides their proteoforms implicated the pathogenesis of different forms dementia. The neuropeptide VGF is decreased cerebrospinal fluid (CSF) brain patients Alzheimer's disease (AD) VGF356-375 VGF1-40 decrease CSF AD frontotemporal dementia (FTD), respectively,...
The granin family of neurotransmitter-like molecules, neuropeptides, are proteolytically processed into functional peptides, also known as proteoforms. This contains secretogranin VII (VGF), which was highlighted by AMP-AD the most nominated potential therapeutic target for Alzheimer's disease (AD) due to its levels being decreased in CSF samples and brain sections from patients with AD. Full-length granins their proteoforms involved diverse neuronal synaptic functions, but physiological...
Abstract Background: COVID-19 has resulted in significant morbidity and mortality worldwide. Lateral flow assays can detect anti-Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) antibodies to monitor transmission. However, standardized evaluation of their accuracy tools aid interpreting results are needed. Methods: We evaluated 20 IgG IgM selected from available tests April 2020. the assays’ performance using 56 pre-pandemic negative SARS-CoV-2-positive plasma samples, collected...