A5100704571- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- T-cell and B-cell Immunology
- Mechanisms of cancer metastasis
- Viral Infectious Diseases and Gene Expression in Insects
- Ion channel regulation and function
- Immune Cell Function and Interaction
- Cancer Mechanisms and Therapy
- Genetic factors in colorectal cancer
- Cancer-related gene regulation
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Liver physiology and pathology
- MicroRNA in disease regulation
- stochastic dynamics and bifurcation
- Cardiac electrophysiology and arrhythmias
- Autophagy in Disease and Therapy
Tongji University
2015-2025
Kunming Medical University
2024
First Affiliated Hospital of Kunming Medical University
2024
Tongji Hospital
2023
State Key Laboratory of Medical Neurobiology
2011
Fudan University
2011
Utah AIDS Foundation
2010
PLA 306 Hospital
2000
Zhangjiakou Academy of Agricultural Sciences
2000
Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects dopaminergic neurons from damage. In this study, we used MTT, immunohistochemistry, and TUNEL staining to investigate the protective effect of MANF in SH-SY5Y cells treated with 6-OHDA or overexpressed α-synuclein. Cleaved caspase-3 levels significantly increased α-synuclein overexpression that induced cleaved increase was reduced by treatment. addition, treatment upregulated GRP78 expressions α-synuclein, RNAi knockdown for...
Abstract Cancer cells release DNA fragments into plasma as circulating free (cfDNA). However, quantitative measurement of tumor‐derived in cfDNA remains challenge. The purpose this study was to quantitatively assess lung cancer patients. By optimizing competitive allele‐specific TaqMan PCR (CAST‐PCR), we assessed the copy number mutated Kirsten rat sarcoma viral oncogene homolog (KRAS) and epidermal growth factor receptor (EGFR) alleles pre/post surgery 168 An absolute method developed total...
Since tumors are often infiltrated by macrophages, it would be advantageous to turn these types of cells into cytotoxic effector cells. Here, we have designed a novel bispecific antibody (BsAb) that targets both tumor antigen (CD20) and the FcαRI receptor (CD89). This could used lyse connecting CD89-expressing immune such as macrophages neutrophils. Previously there were very limited attempts exploit FcαRI-expressing for cell-killing, largely due lack an appropriate in vivo model, since mice...
Chinese hamster ovary (CHO) cells are frequently used for the production of recombinant proteins therapeutical applications. However, protein expression level CHO may reduce during long-term culture. The physiological changes related to stability were not well understood. In this study, we performed a series genetic analysis on stable and unstable clonal derived populations. Transcriptome revealed that large number differentially expressed genes (>100) identified in population between early...
B7-H3 plays an important role in tumor apoptosis, proliferation, adhesion, angiogenesis, invasion, migration, and evasion of immune surveillance. It is overexpressed various human solid tissues. In patients, overexpression correlates with advanced stages, poor clinical outcomes, resistance to therapy. The roles progression make it a potential candidate for targeted Here, we generated mouse anti-human antibody demonstrated its binding activity via Tongji University Suzhou...
Immunotherapy is an effective method for tumour treatment. Anti-programmed cell death protein 1 (PD-1) and anti-programmed death-ligand (PD-L1) monoclonal antibodies play a significant role in immunotherapy of most tumours; however, some patients develop drug resistance to PD-1/PD-L1 therapy. Cyclooxygenase-2 (COX2) expressed various solid tumours, prostaglandin E2 (PGE2) drives the development malignant tumours. We developed drug-resistant B16F10 (B16F10-R) mouse model through four rounds...
Neoantigen vaccines are one of the most effective immunotherapies for personalized tumour treatment. The current immunogen design neoantigen is usually based on whole-genome sequencing (WGS) and bioinformatics prediction that focuses binding affinity between peptide MHC molecules, ignoring other peptide-presenting related steps. This may result in a gap high accuracy relatively low clinical effectiveness. In this study, we designed an integrated in-silico pipeline, Neo-intline, which started...
AA (arachidonic acid), which possesses both neurotoxic and neurotrophic activities, has been implicated as a messenger in physiological pathophysiological processes. In the present study, we investigated effects of extracellular intracellular application on activity Na(V) (voltage-gated Na(+) channels) rat cerebellar GCs (granule cells). The inhibited resultant I(Na) (Na(V) current), wherein current-voltage curve shifted to negative voltage direction. Because this effect could be reproduced...
Therapeutic antibodies are effective for tumor immunotherapy and exhibit prominent clinical effects. All approved antibody therapeutics utilize IgG as the molecular format. Antibody-dependent cell-mediated cytotoxicity (ADCC) is a key mechanism cell killing by antibodies. For antibodies, ADCC depends on FcγR-expressing cells, such natural killer (NK) cells. However, in patients with high burden, may lose efficacy owing to exhaustion of effector cells well inhibitory effects certain FcγRs To...
Purpose: Early diagnosis of lung cancer is critical to curtailing cancer-related deaths.We aimed develop a highly sensitive assay for the analysis circulating tumor DNA (ctDNA) detect non-small cell (NSCLC) in early stages.Materials and Methods: We detected EGFR KRAS mutations paired plasma tissue samples from 147 NSCLC patients.Of these, EGFR/KRAS ctDNA protein biomarkers were comparatively analyzed 87 individuals.In addition, 20 patients subjected repeat multi-gene detection, pre-and...
Although IgA nephropathy (IgAN) is the most common primary glomerulonephritis worldwide, its etiology remains only partly understood. It clear that pathogenesis of IgAN involves formation macromolecular IgA1 complexes and increased levels serum IgA1-immune complexes(IC), due to defective clearance. Previous studies suggest blood tissue myeloid cell-expressed Fc receptor (FcαR/CD89) mediates IgA-IC clearance dysfunction, via decreased activity or excessive soluble FcαR/sCD89 induces IgAN....
Introduction Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease. Anti-B-cell-activating factor (BAFF) therapy effectively depletes B cells and reduces SLE disease activity. This research aimed to evaluate the effect of BAFF blockade on cell receptor (BCR) repertoire gene expression. Methods Through next-generation sequencing, we analyzed expression BCR in MRL/lpr mice that received long-term anti-BAFF therapy. Based profiles, predicted relative proportion immune using...
Despite the survival benefits observed with immune checkpoint blockade (ICB) treatment-programmed cell death-1/programmed death ligand-1 (PD-1/PD-L1), many patients cancer have not benefited from these agents because of impaired antigen presentation and other resistance mechanisms. To overcome to therapy, we designed bispecific antibodies (BsAbs) targeting CD89 tumor antigens. We demonstrated their immunomodulatory efficacy as a separate treatment strategy or combined inhibitors. previously...
Introduction: Overexpression of c-Met, or hepatocyte growth factor (HGF) receptor, is commonly observed in tumor biopsies and often associated with poor patient survival, which makes HGF/c-Met pathway an attractive molecular target for cancer therapy. A number antibody-based therapeutic strategies have been explored to block c-Met HGF cancers; however, clinical efficacy has very limited, indicating that blockade signal alone not sufficient. Thus, alternative approach develop immunotherapy...
Arachidonic acid (AA) and its metabolic products are important second messengers which exert many biological actions, including modulation of various ion channels. However, the blockage muscle Na(+) channel isoforms by AA has not been examined in detail. Here, we investigated modulating effects on rNa(V)1.4 expressed human embryonic kidney 293 cells. The results revealed that both activation inhibitory currents depending depolarizing potential: increased current evoked a depolarization -30...