A5078651768- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Epigenetics and DNA Methylation
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Plant Pathogens and Resistance
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Cancer-related gene regulation
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Plant Molecular Biology Research
- GABA and Rice Research
- Genetic diversity and population structure
- Banana Cultivation and Research
- African Botany and Ecology Studies
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Spectroscopy and Laser Applications
- Intracerebral and Subarachnoid Hemorrhage Research
- Moyamoya disease diagnosis and treatment
Kasugai Municipal Hospital
2024
Saiseikai Kumamoto Hospital
2021
RIKEN
2000-2021
RIKEN Center for Advanced Photonics
2020-2021
Pioneer (Japan)
2020
RIKEN Advanced Science Institute
2007-2012
Laboratoire de Biochimie
2007
RIKEN Center for Integrative Medical Sciences
2006
Roswell Park Comprehensive Cancer Center
2005
Cancer Genetics (United States)
2005
Early studies proposed that DNA methylation could have a role in regulating gene expression during development [Riggs, A.D. (1975) Cytogenet. Cell Genet. 14, 9–25]. However, some of known tissue-specific genes do not support major for methylation. In the results presented here, differentially methylated regions (TDMs) were first identified, and then associated with these correlated status. Restriction landmark genomic scanning (RLGS) was used conjunction virtual RLGS to identify 150 TDMs...
Many CpG islands have tissue-dependent and differentially methylated regions (T-DMRs) in normal cells tissues. To elucidate how DNA methyltransferases (Dnmts) participate methylation of the genomic components, we investigated genome-wide pattern T-DMRs with Dnmt1 -, Dnmt3a and/or Dnmt3b -deficient ES by restriction landmark scanning (RLGS). Approximately 1300 spots were detected wild-type cells. In -/- cells, additional 236 emerged, indicating that corresponding loci are Intriguingly, same...
Male-associated DNA sequences were analyzed in Cannabis sativa L. (hemp), a dioecious plant with heteromorphic sex chromosomes. was isolated from male and female plants subjected to random amplified polymorphic analysis. Of 120 primers, 17 yielded 400 1500-bp fragments detectable male, but not female, plants. These cloned used as probes gel-blot analysis of genomic DNA. When hybridized 2 these male-specific fragments, MADC(male-associated C. sativa)3 MADC4, particularly intense bands...
Heavy-ion irradiation is a new method of mutation breeding to produce cultivars. We established the application this in rice plants obtain mutants. Rice seeds were irradiated by C or Ne ions (135MeV/u) with LET (linear energy transfer) 22.7 64.2 keV/μm, respectively. Chlorophyll-deficient mutants (CDM) segregated M2 progeny albino, pale-green, yellow striped-leave phenotypes. The highest rate CDM C-ion irradiation, 7.31%, was obtained at 40 Gy among doses examined. Ne-ion gave rate, 11.6%,...
The surgical techniques for treatment of chronic subdural hematoma (CSDH), a common neurosurgical condition, have been discussed in lot clinical literature. However, the recurrence proportion after CSDH surgery remains high, ranging from 10 to 20%. standard procedure involves craniostomy evacuate hematoma, but irrigating cavity during is debatable. authors hypothesized that choice irrigation fluid might be key factor affecting outcomes surgery. This multicenter randomized controlled trial...
Understanding the role of 'epigenetic' changes such as DNA methylation and chromatin remodeling has now become critical in understanding many biological processes. In order to delineate global pattern a given genomic DNA, computer software been developed create virtual image restriction landmark scanning (Vi-RLGS). When using methylation- sensitive enzyme NotI landmark, comparison between real silico RLGS profiles genome provides map sites. A Arabidopsis was created that could be confirmed...
CMA/DAPI double staining and FISH methods using rDNA telomere specific repeated sequences as probes were applied to characterize the large amount of heterochromatin in telomeric regions 'Trovita' orange (Citrus sinensis Osbeck) chromosomes. As a result, all heterochromatic CMA+/DAPI–, thus GC rich. Three CMA+/DAPI– positive 26S probe. Telomere arrays consisting T m (A)G n detected on extreme ends each chromosome most CMA+/DAPI – ordered close inside telomere-specific sequences. The data...
The DNA adenine methylation status on specific 5′‐GANTC‐3′ sites and its change during the establishment of plant–microbe interactions was demonstrated in several species α‐proteobacteria. Restriction landmark genome scanning (RLGS), which is a high‐resolution two dimensional electrophoresis method, used to monitor genomewide methylation. In case Mesorhizobium loti MAFF303099, real RLGS images obtained with restriction enzyme Mbo I, digests at GATC sites, almost perfectly matched virtual...
We have developed an efficient system to detect and analyze DNA mutations induced by heavy-ion beams in Arabiopsis thaliana. In this system, a stable transgenic Arabidopsis line that constitutively expresses yellow fluorescent protein (YFP) single-copy gene at genomic locus was constructed irradiated with beams. The YFP is target of mutagenesis, its loss function or expression can easily be detected the disappearance signals planta under microscopy. With 12C6+-induced mutant single deletion...
Many plants, including fruits and vegetables, release biogenic gases containing various volatile organic compounds such as ethylene (C2H4), which is a gaseous phytohormone. Non-destructive in-situ gas sampling technology to detect trace C2H4 released from plants in real time would be attractive for visualising the ageing, ripening, defence reactions of plants. In this study, we developed detection system with limit 0.8 ppb (3σ) using laser absorption spectroscopy. The consists mid-infrared...
Objective Ceftriaxone (CTRX) is a widely used antibiotic because of its long plasma half-life and good tissue transmission. Many the reported studies on CTRX-associated pseudolithiasis were performed in children. Although some have been published adults, there are no limited to elderly people. The present study investigated explored risk factors elderly. Methods We retrospectively reviewed 133 patients (≥65 years old) treated with CTRX. Pseudolithiasis was defined as stones or sludge newly...