A5042398070- X-ray Diffraction in Crystallography
- Antifungal resistance and susceptibility
- Crystallization and Solubility Studies
- Fungal Infections and Studies
- Nanoparticles: synthesis and applications
- Probiotics and Fermented Foods
- Prion Diseases and Protein Misfolding
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Essential Oils and Antimicrobial Activity
- Neonatal and Maternal Infections
- Phytochemistry and Bioactivity Studies
- Metal complexes synthesis and properties
- Oral Health Pathology and Treatment
- Phytochemicals and Antioxidant Activities
- Lichen and fungal ecology
- Nail Diseases and Treatments
- Bacterial Genetics and Biotechnology
- Genetic and phenotypic traits in livestock
- Immunotherapy and Immune Responses
- Animal Genetics and Reproduction
- Synthesis and Characterization of Heterocyclic Compounds
- Yeasts and Rust Fungi Studies
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
University of Veterinary Medicine in Košice
2015-2024
Institute of Immunology
2009-2012
Czech Academy of Sciences, Institute of Microbiology
2001-2007
University of Veterinary Medicine Vienna
2000-2006
Slovak Academy of Sciences
1999-2005
Institute of Neuroimmunology of the Slovak Academy of Sciences
2004-2005
Beijing Children’s Hospital
2004
University of Tripoli
2001
Veterinary Research Institute
2001
University of Veterinary Medicine
2001
A green synthetic route for the production of silver nanoparticles (AgNPs) using five different aqueous plant extracts, namely, Berberis vulgaris, Brassica nigra, Capsella bursa-pastoris, Lavandula angustifolia and Origanum vulgare, was investigated in this study. The present work demonstrates influence extract composition (antioxidant total phenolic content) on size morphology produced AgNPs. biosynthetic procedure rapid simple easily monitored via colour changes ultraviolet visible...
Lichen metabolites represent a wide range of substances with variety biological effects. The present study was designed to analyze the potential antiproliferative, antimicrobial and antioxidative effects several extracts from lichens (Pseudevernia furfuracea, Lobaria pulmonaria, Cetraria islandica, Evernia prunastri, Stereocaulon tomentosum, Xanthoria elegans Umbilicaria hirsuta) their secondary (atranorin, physodic acid, evernic acid gyrophoric acid). crude extract, as well isolated...
In the present study, a quick and environmentally friendly approach has been developed for synthesis of CuS nanocrystals different shape with antibacterial activity. The process is completed in 5 min. presence copper sulfide final products was confirmed by XRD, Raman spectroscopy, XPS, TEM. morphology product dependent on whether or not reaction providing sulfur realized. When included, particles an elongated thickness range between 6 8 nm lengths up to 60 nm. It found that plays role...
Abstract A bionanocomposite containing Ag/AgCl nanoparticles and plant biomass was prepared via solvent-free biomechanochemical synthesis utilizing planetary ball milling of lavender silver nitrate powders in this work. The impact four input parameters, namely speed, time sample mass plant-to-AgNO 3 ratio assessed a ‘Design Experiments methodology’, Taguchi orthogonal array. In way, optimum conditions were identified analysis variance regression performed. obtained Ag AgCl nanocrystalline,...
A completely solid-state mechanochemical synthesis of silver nanoparticles overcoming the problem with water insolubility lichen metabolites hampering their use for classical green in is shown herein. Four species (Xanthoria elegans, Cetraria islandica, Usnea antarctica, and Leptogium puberulum) AgNO3 were used as reducing agents Ag(0) precursor, respectively. The reaction progress was rapid first two cases, whereas case U. antarctica L. puberulum, a significant amount could still be...
This study shows mechanochemical synthesis as an alternative method to the traditional green of silver nanoparticles in a comparative manner by comparing products obtained using both methodologies and different characterization methods. As precursor, most commonly used nitrate was applied easily accessible lavender (Lavandula angustofolia L.) plant reducing agent. Both syntheses were performed 7 lavender:AgNO3 mass ratios. The time limited 8 15 min case synthesis, respectively, although...
The gastrointestinal tract is the largest and most complex component of immune system. Each influences production regulation cytokines secreted by intestinal epithelial cells. aim this study was to see how probiotic strain Limosilactobacillus reuteri L26 its exopolysaccharide (EPS) affect porcine intestinal-epithelial cells IPEC-J2 infected with Salmonella Typhimurium. results revealed that infection up-regulated all studied pro-inflammatory such as TNF-α, IL-8, IL-6 TLR4, TLR5 signaling...
Abstract BACKGROUND Anthocyanins, compounds that represent the major group of flavonoids in berries, are one most powerful natural antioxidants. The aim this study was to evaluate biological activities and comparison anthocyanin‐rich extracts prepared from chokeberry ( Aronia melanocarpa ), elderberry Sambucus nigra bilberry Vaccinium myrtillus ) blueberry V. corymbosum on porcine intestinal epithelial IPEC ‐1 cell line. RESULTS IC 50 values calculated antioxidant cell‐based...
Abstract Green synthesis was successfully used for silver nanoparticles (Ag NPs) preparation using Berberis vulgaris aqueous extracts. The ultraviolet and visible (UV–Vis) spectroscopy, photon cross-correlation spectroscopy (PCCS), transmission electron microscopy (TEM) selected area diffraction (SAED) techniques were characterization of the Ag NPs confirmed presence Ag(0) in nanoparticles. bimodal morphology discovered, namely, larger particles had elongated shape size around 200 nm, while...
Twelve Cu-based ternary (Cu–Me1–S, Me1 = Fe, Sn, or Sb) and quaternary (Cu–Me2–Sn–S, Me2 Zn, V) nanocrystalline sulfides are shown as perspective antibacterial materials here. They were prepared from elemental precursors by a one-step solvent-free mechanochemical synthesis in 100 g batch using scalable eccentric vibratory ball milling. Most of the products have strong activity against Escherichia coli Staphylococcus aureus bacteria. For instance, stannite Cu2FeSnS4 mohite Cu2SnS3 most active...
Two tetranuclear [Zn4Cl2(ClQ)6]·2DMF (1) and [Zn4Cl2(ClQ)6(H2O)2]·4DMF (2), as well three dinuclear [Zn2(ClQ)3(HClQ)3]I3 (3), [Zn2(dClQ)2(H2O)6(SO4)] (4) [Zn2(dBrQ)2(H2O)6(SO4)] (5), complexes (HClQ = 5-chloro-8-hydroxyquinoline, HdClQ 5,7-dichloro-8-hydroxyquinoline HdBrQ 5,7-dibromo-8-hydroxyquinoline) were prepared possible anticancer or antimicrobial agents characterized by IR spectroscopy, elemental analysis single crystal X-ray structure analysis. The stability of the in solution was...
The aim of the present study was to compare concentrations two acute phase proteins, haptoglobin (Hp) and serum amyloid A (SAA), in milk Holstein-Friesian dairy cows grouped according somatic cell count thresholds (< 100,000 < 400,000 cells/ml) composite samples. SCC assessed quantitatively by FOSSOMATIC 90 analyser; Hp SAA were determined using commercial ELISA kits (Tridelta Development, Ltd., Wicklow, Ireland). We have found a significantly higher group with > cells/ml compared...
An effort to prepare different non-stoichiometric CuxSy compounds starting from elemental precursors using mechanochemistry was made in this study. However, out of the 7 stoichiometries tested, it only possible obtain three phases: covellite CuS, chalcocite Cu2S and digenite Cu1.8S their mixtures. To phase with highest purity, Cu : S stoichiometric ratio needed be fixed at 1.6 1. The reaction between copper sulfur completed within a second range, however, milling performed for up 15 minutes...
Silver nanoparticles (Ag NPs) with antibacterial activity can be prepared in different ways. In our case, we used ecological green synthesis Agrimonia eupatoria L. The plant extract was Ag NPs for the first time to prepare termosensitive situ gels (ISGs). Such are heal human or animal skin and mucous membranes, as they change from a liquid solid state after application. were characterized various techniques (FTIR, TEM, size distribution, zeta potential) their tested against Staphylococcus...
Abstract The aim of this study was to evaluate the immunomodulatory effect EPS-L26 isolated from probiotic strain Lactobacillus ( Limosilactobacillus ) reuteri L26 Biocenol™, in a model infection with an enterotoxigenic E. coli (ETEC) by establishing monocultures consisting IPEC-J2 cell line or monocyte-derived dendritic cells (moDCs) and creating 3D co-cultures established moDCs. immunoprotective potential used confirmed experimental group pretreated cells, where our showed that...
The aim of the study was to determine prevalence genes encoding virulence factors in Staphylococcus aureus strains isolated from raw sheep milk, cheese and Bryndza cheese. Genes staphylococcal enterotoxin ( sea , seb sec sed see ), toxic shock syndrome toxin-1 tst exfoliative toxins eta etb ) collagen-binding protein cna were detected. In a total 79 S. isolates all assessed found, except for . Overall, 75.9% found be positive one or more toxin genes. gene most frequently (24.1%), followed by...