A5057972117- Cellular transport and secretion
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Retinoids in leukemia and cellular processes
- Neuroscience and Neuropharmacology Research
- Nuclear Receptors and Signaling
- Parkinson's Disease Mechanisms and Treatments
- HIV Research and Treatment
- Microtubule and mitosis dynamics
- SARS-CoV-2 detection and testing
- CRISPR and Genetic Engineering
- Alzheimer's disease research and treatments
- Cardiomyopathy and Myosin Studies
- Cell Image Analysis Techniques
- Nuclear Structure and Function
- Ion Transport and Channel Regulation
- Biosensors and Analytical Detection
- Molecular Communication and Nanonetworks
- Connexins and lens biology
- Hippo pathway signaling and YAP/TAZ
- Autophagy in Disease and Therapy
- Single-cell and spatial transcriptomics
- Signaling Pathways in Disease
- ATP Synthase and ATPases Research
- Gene Regulatory Network Analysis
Stony Brook University
2013-2024
Worcester Polytechnic Institute
2019-2024
Queen's University
2024
University of Washington
2017-2018
Drp1 (dynamin-related protein 1), a large GTPase, mediates the increased mitochondrial fission, which contributes to hyperproliferation of pulmonary artery smooth muscle cells in arterial hypertension (PAH). We developed potent GTPase inhibitor, Drpitor1a, but its specificity, pharmacokinetics, and efficacy PAH are unknown.
Introduction: Dynamin-related protein 1 (Drp1) activation increases mitochondrial fission and cell cycle progression in hyperproliferative cells, termed mitotic fission. However, the diameter of a apparatus comprised solely Drp1 its binding partners appears insufficient to complete Moreover, mechanism linking is unknown, suggesting an additional mediator terminal steps Hypothesis: The large GTPase, dynamin 2 (DNM2), interacts with regulate progression. Corollary: DNM2 epigenetically...
The Endosomal Sorting Complex Required for Transport (ESCRT) is an evolutionarily conserved machinery that performs reverse-topology membrane scission in cells universally required from cytokinesis to budding of enveloped viruses. Upstream acting ESCRT-I and ALIX control these events link recruitment viral cellular partners late-acting ESCRT-III CHMP4 through incompletely understood mechanisms. Using structure-function analyses combined with super-resolution imaging, we show function as...
Abstract IQGAP1 is a multi-domain protein that acts as scaffold for multiple signaling pathways. generates the lipid messenger PI(3,4,5)P 3 by scaffolding phosphoinositide kinases PIPKIs and PI3K. The dynamics of this complex in intact, living cells are unknown. Here, we delineate role -mediated live under basal stimulated conditions using fluorescence lifetime imaging microscopy. We demonstrate interacts strongly with PIPKIγ at intracellular entities on plasma membrane, scaffolds PI3K...
Abstract RNAs mediate many different processes that are central to cellular function. The ability quantify or image in live cells is very useful elucidating such functions of RNA. RNA aptamerfluorogen systems have been increasingly used labeling cells. Here, we use the malachite green aptamer (MGA), an can specifically bind (MG) dye and induces it emit far-red fluorescence signals. Previous studies on MGA showed a potential for genetically tagging other molecules However, these also...
Abstract We describe a rapid and reusable biophysical method to assay COVID-19 other pathogens. The uses fluorescent sensors (i.e. molecular beacons) designed detect RNA or any of interest, concurrent with an internal control without the need for amplification. beacons are stem-loop structures in which ∼10 nucleotide loop region has complementary sequence target RNA, fluorophore quencher placed on 5’ 3’ ends stem. energy hybridization its is be greater than stem so that when beacon...
We describe a rapid and reusable biophysical method to assay COVID-19. The uses fluorescent sensors (i.e., molecular beacons) designed detect specific RNA sequence from COVID-19 but is general any of interest. can be used concurrently with an internal control without the need for amplification. Molecular beacons are stem-loop structures in which ~10 nucleotide loop region has complementary target RNA, fluorophore quencher placed on 5’ 3’ ends stem. energy hybridization its greater than stem...
Abstract IQGAP1 is a large, multi-domain scaffold that connects and modulates different signaling networks including the one initiated by epidermal growth factor (EGF)by localizing concentrating pathway components to promote activation. In this study, we have used live cell fluorescence imaging methods along with other biochemical techniques follow mechanisms enhance EGF signaling. We show enhances promoting oligomerization of its receptor, EGFR upon addition concurrent IQGAP...
Abstract Cells process extra-cellular signals with multiple layers of complex biological networks. Due to the stochastic nature networks, become significantly noisy within cells and in addition, due nonlinear distorted, shifted, (de-)amplified. Such signal processing can lead non-trivial cellular phenotypes such as cell cycles, differentiation, cell-to-cell communication, homeostasis. These pheno-types, when observed at population levels, be quite different from single-cell level...