A5067332743- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Resistance
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Cassava research and cyanide
- Fern and Epiphyte Biology
- Seed Germination and Physiology
- Soybean genetics and cultivation
- Agronomic Practices and Intercropping Systems
- Plant Disease Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogens and Fungal Diseases
- Polysaccharides and Plant Cell Walls
- Agriculture, Plant Science, Crop Management
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Plant Growth Enhancement Techniques
- Plant Pathogenic Bacteria Studies
- Ubiquitin and proteasome pathways
- Plant Disease Management Techniques
- Transgenic Plants and Applications
Warsaw University of Life Sciences
2016-2025
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2020
Polish Academy of Sciences
2020
University of Warsaw
2013
University of Vienna
2007
BOKU University
2007
Kiel University
1994-1998
Plants lack the seemingly unlimited receptor diversity of a somatic adaptive immune system as found in vertebrates and rely on only relatively small set innate receptors to resist myriad pathogens. Here, we show that disease-resistant tomato plants use an efficient mechanism leverage limited nonself recognition capacity their system. We extracellular plant protein Cf-2 red currant ( Solanum pimpinellifolium ) has acquired dual resistance specificity by sensing perturbations common virulence...
Significance Sedentary plant-parasitic cyst nematodes are microscopic roundworms that cause significant yield losses in agriculture. Successful parasitism is based on the formation of a hypermetabolic feeding site host roots from which withdraw their nutrients. The cell cycle activated at infection and contributes to syncytium. Here, we provide genetic evidence nematode-derived cytokinin involved activating during infection. Our findings show ability an animal synthesize secrete functional...
Reactive oxygen species restrict cell death at sites of parasitic nematode infection and support nurse formation in plant roots.
Plant-parasitic nematodes (PPNs) cause tremendous yield losses worldwide in almost all economically important crops. The agriculturally most PPNs belong to a small group of root-infecting sedentary endoparasites that includes cyst and root-knot nematodes. Both induce specialized long-term feeding structures root vasculature from which they obtain their nutrients. A cell layer roots called the endodermis, has walls reinforced with suberin deposits lignin-based Casparian strip (CS), protects...
Abstract Plant-parasitic nematodes are a major threat to crop production in all agricultural systems. The scarcity of classical resistance genes highlights pressing need find new ways develop nematode-resistant germplasm. Here, we sequence and assemble high-quality phased genome the model cyst nematode Heterodera schachtii provide platform for first system-wide dual analysis host parasite gene expression over time, covering parasitism stages. Analysis hologenome plant-nematode infection site...
The tomato Hero A gene is the only member of a multigene family that confers high level (>80%) resistance to all economically important pathotypes potato cyst nematode (PCN) species Globodera rostochiensis and G. pallida. Although levels transgenic lines were similar those line LA1792 containing introgressed family, plants expressing are not resistant PCNs. Comparative microscopy studies in vitro infected roots PCN-susceptible cv. Money Maker, breeding LA1792, L10 with Ro1 pathotype have...
Successful biotrophic plant pathogens can divert host nutrition toward infection sites. Here we describe how the protist Plasmodiophora brassicae establishes a long-term feeding relationship with its by stimulating phloem differentiation and phloem-specific expression of sugar transporters within developing galls. Development galls in infected Arabidopsis (Arabidopsis thaliana) plants is accompanied stimulation BREVIS RADIX, COTYLEDON VASCULAR PATTERN, OCTOPUS gene leading to an increase...
As obligate photoautotrophs, plants are inevitably exposed to ultraviolet (UV) radiation. Because of stratospheric ozone depletion, UV has become more and dangerous the biosphere. Therefore, it is important understand perception signal transduction in plants. In present study, we show that lesion simulating disease 1 (LSD1) enhanced susceptibility (EDS1) antagonistic regulators UV-C-induced programmed cell death (PCD) Arabidopsis thaliana. This regulatory dependence manifested by a complex...
Summary Sedentary plant‐parasitic cyst nematodes are obligate biotrophs that infect the roots of their host plant. Their parasitism is based on modification root cells to form a hypermetabolic syncytium from which draw nutrients. The aim this study was identify nematode susceptibility genes in Arabidopsis thaliana and characterize roles supporting Heterodera schachtii . By selecting were most strongly upregulated response infection, we identified HIPP27 ( H EAVY METAL‐ASSOCIATED I...
Photosynthetic efficiency and redox homeostasis are important for plant physiological processes during regular development as well defence responses. The second-stage juveniles of Heterodera schachtii induce syncytial feeding sites in host roots. To ascertain whether the syncytia alters photosynthesis metabolism reactive oxygen species (ROS), chlorophyll a fluorescence measurements antioxidant responses were studied Arabidopsis thaliana shoots on day inoculation at 3, 7 15 days...
‘Green island’ symptoms in the form of vivid green, round spots visible on senescent leaves many plants and trees are mostly results pathogenic colonization by fungi, greenish tissue is often dead. Therefore, this study investigates whether green observed Norway maple (Acer platanoides L.) were still alive photosynthetically active. The appearance ‘green islands’ young was from autumn 2019 to 2022 an urban forest (Bialystok, eastern Poland). However, late summer (September) 2023 2024, tar...
The cyst nematode Heterodera schachtii infects roots of Arabidopsis plants and establishes feeding sites called syncytia, which are the only nutrient source for nematodes. Development syncytia is accompanied by changes in cell wall structures including development ingrowths. UDP-glucuronic acid a precursor several polysaccharides can be produced UDP-glucose dehydrogenase through oxidation UDP-glucose. Four genes encode this enzyme. Promoter::GUS analysis revealed that UGD2 UGD3 were...
Summary The enzyme myo‐inositol oxygenase is the key of a pathway leading from to UDP ‐glucuronic acid. In A rabidopsis, encoded by four genes. All genes are strongly expressed in syncytia induced beet cyst nematode H eterodera schachtii rabidopsis roots. Here, we studied effect quadruple mutant on development. We performed metabolite profiling roots mutant. role galactinol was using lines with elevated levels and supplying wild‐type seedlings. showed significant reduction susceptibility . ,...
The beneficial endophytic fungus Piriformospora indica colonizes the roots of many plant species, including model Arabidopsis thaliana. Its colonization promotes growth, development, and seed production as well resistance to various biotic abiotic stresses. In present work, P. was tested potential antagonist sedentary plant-parasitic nematode Heterodera schachtii. This biotrophic cyst-forming induces severe host damage by changing morphogenesis physiology infected roots. Here it is shown...
Abstract Hydrogen peroxide (H2O2) production during the infection of Arabidopsis thaliana by soybean cyst nematode Heterodera glycines was detected histochemically reaction H2O2 with cerium chloride producing four different patterns electron-dense precipitates perhydroxides. As A. is not a regular host H. glycines, defence response considerable, but does completely inhibit development nematode. produced only cells mechanically damaged invasion and feeding site induction nematode, also...
Abstract The plant parasitic nematode Heterodera schachtii induces specific syncytial feeding sites in the roots of Arabidopsis thaliana from where it withdraws all required nutrients. Therefore, syncytia have to be well supplied with assimilates and generate strong sinks host plant's transport system. Import mechanisms consequent accumulation sucrose were described recently. In this work, we studied starch metabolism syncytia. Using high-performance liquid chromatography microscopic...
Abstract The plant-parasitic nematode Heterodera schachtii is an obligate biotroph that induces syncytial feeding sites in roots of its hosts. Nematodes produce effectors are secreted into the host and facilitate infection process. Here we identified H. protein disulphide isomerase (HsPDI) as a putative effector interferes with host’s redox status. In situ hybridization showed HsPdi specifically localized within esophageal glands pre-parasitic second stage juveniles (J2). up-regulated early...
Pollen development is a strictly controlled post-meiotic process during which microspores differentiate into microgametophytes and profound structural functional changes occur in organelles. Annexin 5 calcium- lipid-binding protein that highly expressed pollen grains regulates physiology. To gain further insights the role of ANN5 Arabidopsis development, we performed detailed phenotypic characterization plants with modified levels. In addition, interaction partners subcellular localization...