A5019236345- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- Cancer-related gene regulation
- Circular RNAs in diseases
- Animal Virus Infections Studies
- Muscle Physiology and Disorders
- Virology and Viral Diseases
- RNA modifications and cancer
- PARP inhibition in cancer therapy
- Biochemical Analysis and Sensing Techniques
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Prostate Cancer Treatment and Research
- Viral Infections and Vectors
- Genetic and phenotypic traits in livestock
- Bacterial Genetics and Biotechnology
- Olfactory and Sensory Function Studies
- Nanopore and Nanochannel Transport Studies
- Reproductive Biology and Fertility
Guizhou University
2016-2025
Taiyuan Institute of Technology
2025
Guiyang University
2018-2024
Ministry of Education of the People's Republic of China
2022
Guiyang Medical University
2022
Institute of Molecular and Cell Biology
2018
Institut Curie
2009
Centre National de la Recherche Scientifique
2009
This study aimed to identify polymorphisms in the gene encoding 3-hydroxy-3-methylglutaryl-CoA synthase 1, HMGCS1, and analyze their association with slaughter characteristics, meat quality, organ coefficients Guizhou white goats. A total of 153 twelve-month-old goats (78 male 75 female) were included study. Slaughter assessed. Association analyses between genotypes phenotypic traits conducted using a generalized linear model. Four polymorphic loci identified, i.e., g.15523T>C, g.15530G>C,...
Background: Pigs are vital agricultural animals, with growth traits serving as key indicators of their quality. Methods: In this study, we examined the mRNA expression ENPP1 a candidate gene in heart, liver, spleen, lungs, and kidneys at 3 days 6 months age by real-time polymerase chain reaction method single-nucleotide polymorphism (SNP) loci 165 Xiangsu pigs Sanger sequencing. Results: The different tissues stages was significantly different, it had high conservation species.. Sequence...
<title>Abstract</title> Dehydrogenase/Reductase (SDR) Family Member 3 (<italic>DHRS3</italic>, also known as Rsdr1 and retSDR1) is a membrane-bound enzyme with critical roles in embryonic development animal growth. Studying the porcine <italic>DHRS3</italic> gene promoter can provide foundation for understanding growth, development, reproduction pigs. In this study, we first analyzed region structure of constructed five dual-luciferase reporter vectors sequential deletion fragments. Through...
Prostate cancer (PCa) ranks as the second most prevalent among males globally. However, exact mechanisms underlying its progression remain inadequately elucidated. The present study sought to investigate role and molecular mechanism of hsa_circ_0001671 (circ_0001671) in pathogenic behavior PCa cells. Guided by ceRNA theory, miR-27b-3p was employed identify circRNAs that could potentially regulate Bloom Syndrome Protein (BLM). A series experimental approaches including bioinformatics,...
Taste receptor type 1 subunit 3 (T1R3) is initially expressed in mammal tongue for recognition and response of sweet/umami tastants critical to nutrient absorption, even endocrine. In this study, down-regulation related steroidogenic enzymes such as StAR, 3β-HSD, CYP17A1, 17β-HSD with the decrease T1R3 expression was found Leydig cells treated by a inhibitor (lactisole). The abundances progesterone, 17a-hydroxyprogesterone, androstenedione, testosterone, deoxycorticosterone were...
The matrix (M) protein of Newcastle disease virus (NDV) is demonstrated to localize in the nucleus via intrinsic nuclear localization signal (NLS), but cellular proteins involved import NDV M and role M's replication pathogenicity remain unclear. In this study, importin β1 was screened interact with by yeast two-hybrid screening. This interaction subsequently confirmed co-immunoprecipitation pull-down assays. vitro binding studies indicated that NLS region amino acids 336-433 belonged RanGTP...
BLM RecQ like helicase (BLM) has a pivotal role in genetic recombination, transcription, DNA replication and repair, which presents the possibility of using as an anti‑cancer target for treatment. However, post‑transcriptional control regulation gene expression is not fully understood limits application drugs targeting carcinoma therapy future. MicroRNAs (miRNAs) inhibit through interaction with 3' untranslated region (3'‑UTR) mRNA at stage. Therefore, current study screened miRNAs that...
Prostate cancer (PCa) is one of the most common malignancies, and almost all patients with advanced PCa will develop castration-resistant prostate (CRPC) after receiving endocrine therapy. Effective treatment for CRPC has not been established. Novel approaches are needed to identify therapeutic targets CRPC.Recent research studies have found that members 14-3-3 family play an important role in development progression PCa. Previous results shown ɛ significantly upregulated several cancers....
Testicular expression of taste receptor type 1 subunit 3 (T1R3), a sweet/umami receptor, has been implicated in spermatogenesis and steroidogenesis mice. We explored the role testicular T1R3 porcine postnatal development using Congjiang Xiang pig, rare Chinese miniature pig breed. Based on weights, morphology, testosterone levels, four key developmental stages were identified at days 15–180 (prepuberty: 30 day; early puberty: 60 late 90 sexual maturity: 120 day). During development,...
Prostate cancer (PCa) is one of the most fundamental causes cancer‑related mortality and morbidity among males. However, underlying mechanisms have not yet been fully clarified. The present study aimed to investigate effects plasmacytoma variant translocation 1 (PVT1) on malignant behaviors PCa cells explore possible molecular involved. expression levels PVT1 microRNA (miRNA/miR)‑27b‑3p in tissues cell lines were measured using reverse‑transcritpion‑quantitative polymerase chain reaction....
Bloom's syndrome (BS) is a rare human autosomal recessive disorder characterized by strong predisposition to wide range of cancers commonly affecting the general population. Understanding functioning mechanism BLM protein may provide opportunity develop new effective therapy strategies. In this work, we studied DNA unwinding kinetic helicase core using various stopped-flow assays. We show that unwinds duplex as monomers even under conditions strongly favoring oligomerization. An rate...
Prostate cancer (PC) is a common malignant tumor and leading cause of cancer-related death in men worldwide. In order to design new therapeutic interventions for PC, an understanding the molecular events underlying PC tumorigenesis required. Bloom syndrome protein (BLM) RecQ-like helicase, which helps maintain genetic stability. BLM dysfunction has been implicated development, most recently during tumorigenesis. However, basis BLM-induced progression remains poorly characterized. this study,...
Prostate cancer (PCa) is one of the most common malignant tumours in world and seriously affects health men. Studies have shown that microRNA (miR)-29b-3p tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE) play important roles influencing proliferation apoptosis PCa cells. However, molecular mechanism miR-29b-3p YWHAE cells remains unclear. In present study, bioinformatics as well vivo vitro experiments were used to predict verify targeting relationship...
In this study, Xiangsu hybrid pig growth traits were evaluated via PRKAA2 and MSMB as candidate genes. Sanger sequencing revealed three mutation sites in PRKAA2, namely, g.42101G>T, g.60146A>T, g.61455G>A, all these intronic mutations. Moreover, six identified MSMB: g.4374G>T, exonic g.4564T>C, g.6378G>A, g.6386C>T, g.8643G>A, g.8857A>G. Association analysis that g.8857A>G showed different relationship patterns among body weight, length, height, chest circumference, abdominal tube depth....
This study aimed to explore the regulatory role of FOXO1 promoter methylation on its transcriptional level and unravel effect proliferation differentiation bovine myoblasts. Bisulfite sequencing polymerase chain reaction (BSP) real-time quantitative PCR were performed determine status transcript levels region at different growth stages. BSP results showed that in calf (CB) group was significantly higher than adult (AB) (p < 0.05). On other hand, qRT-PCR indicated mRNA expression AB CB...
Myocyte enhancer factor 2A (MEF2A) is a member of the myocyte 2 family. MEF2A widely distributed in various tissues and organs participates physiological processes. This study aimed to investigate effect expression on proliferation apoptosis bovine myoblasts. CCK8, ELISA, cell cycle, analyses were conducted assess status. In addition, mRNA levels genes associated with myoblast evaluated using RT-qPCR. The results showed that upregulation promoted rate myoblasts, shortened cycle process,...
Abstract In recent years, there has been an increase in the incidence and mortality rates of prostate cancer (PCa). However, specific molecular mechanisms underlying its occurrence development remain unclear, necessitating identification new therapeutic targets. Through bioinformatics analysis, we discovered a previously unstudied differential gene called HIST3H2A cancer. Our study revealed that is highly expressed PCa tissues, as confirmed by analysis both GEO UALCAN databases. Further...