A5041970986- Blood Coagulation and Thrombosis Mechanisms
- Hemophilia Treatment and Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood properties and coagulation
- Vitamin K Research Studies
- Platelet Disorders and Treatments
- Cancer-related gene regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Plant Gene Expression Analysis
- Neonatal and Maternal Infections
- Iron Metabolism and Disorders
- Protease and Inhibitor Mechanisms
- Heparin-Induced Thrombocytopenia and Thrombosis
- Venous Thromboembolism Diagnosis and Management
- Erythrocyte Function and Pathophysiology
- Plant biochemistry and biosynthesis
- Inflammatory Biomarkers in Disease Prognosis
- Systemic Lupus Erythematosus Research
- Cerebral Venous Sinus Thrombosis
- Ion Transport and Channel Regulation
- Machine Learning in Bioinformatics
- Biochemical and biochemical processes
- Mitochondrial Function and Pathology
- Kruppel-like factors research
First Affiliated Hospital of Wenzhou Medical University
2016-2025
Wenzhou Medical University
2016-2025
Guizhou University
2024
First Affiliated Hospital Zhejiang University
2024
Southwest Forestry University
2024
Lishui Central Hospital
2017
Fundación Juan March
2015
Centre de Recherche en Sciences et Technologies de l'Information et de la Communication
2014
IJ Research (United States)
2004
Abstract A crucial aim upon the completion of human genome is verification and functional annotation all predicted genes their protein products. Here we describe mapping peptides derived from accurate interpretations tandem mass spectrometry (MS) data to eukaryotic genomes generation an expandable resource for integration many diverse proteomics experiments. Furthermore, demonstrate that peptide identifications obtained high-throughput can be integrated on a large scale with genome. This...
Analyze the clinical phenotype and gene mutations of a family with hereditary FXII deficiency, preliminarily explore its phenotypic manifestations. The routine coagulation indicators related factors were measured.Thromboelastography thrombin generation tests simulated anticoagulation states in vitro vivo. PCR direct sequencing was utilized to analyze all exons flanking sequences F12 proband, confirming suspected through reverse sequencing, identifying corresponding mutation sites members....
Cerebral venous sinus thrombosis (CVST) is a rare and atypical thrombotic condition, particularly prevalent among young adults, with complex cause. In July October 2023, two patients were diagnosed hereditary protein S deficiency (PSD) presenting CVST at the Department of Neurology, first affiliated hospital Wenzhou Medical University. This study analysed phenotypes gene mutations in PSD pedigrees to investigate link between CVST. A total 11 individuals from these involved. We measured...
Sedum sarmentosum Bunge possesses excellent anti-inflammatory properties and was used in the treatment of inflammatory diseases. The aim present study to investigate efficiency extract (SSBE) on severe acute pancreatitis-associated (SAP-associated) lung injury (ALI) rats explore underlying mechanisms. Here, we a sodium taurocholate-induced SAP rat model determine role SSBE ALI. During course pancreatitis, expressions phosphorylated phosphoinositide 3-kinases (PI3K)/protein kinase B (Akt)...
Abstract Background Factor XI (FXI) deficiency is an autosomal hemorrhagic disorder characterized by reduced plasma FXI levels. Multiple ancestral variants in the F11 gene have been identified Ashkenazi Jews and other selected European populations. However, there are few reports of predominant Chinese and/or East Asian The aim this study to characterize genotypes phenotypes identify variants. Results Of 41 FXI-deficient patients, 39 exhibited severe defects, considerably more than those with...
Objective: To study the gene mutation of human coagulation factor XII (FXII) in a Chinese family with FXII deficiency and it will help us to understand pathogenesis this type disease.Clinical presentation: The proband was 50-year-old male who had fracture right humerus. routine presurgical test showed significant prolonged activated partial thromboplastin time (APTT) at 59.1s (reference range, 29.0–43.0s).Techniques: activity (FXII:C) antigen (FXII:Ag) were detected by one-stage clotting...
Abstract In recent years, there has been an increase in the incidence and mortality rates of prostate cancer (PCa). However, specific molecular mechanisms underlying its occurrence development remain unclear, necessitating identification new therapeutic targets. Through bioinformatics analysis, we discovered a previously unstudied differential gene called HIST3H2A cancer. Our study revealed that is highly expressed PCa tissues, as confirmed by analysis both GEO UALCAN databases. Further...
Congenital factor XII (FXII) de&#xFB01;ciency is a rare autosomal recessive disorder, characterized by great variability in its clinical manifestations. In this study, we screened for mutations the<i> F12</i> gene of 4 unrelated patients with FXII coagulant activity <10% that normal human plasma. To investigate the molecular defects these FXII-deficient patients, performed mutation screening. By sequencing all coding exons as well flanking intronic regions...
Periplocin, a natural compound, has been shown to induce apoptosis in variety of cancer cells. However, no research conducted demonstrate that Periplocin regulatory effect on autophagy. This study is aimed determine the treatment autophagy human pancreatic cells, as well underlying mechanisms. Pancreatic cells were treated with different concentrations and real-time cell analysis (RTCA), colony formation assay, Ki67 immunofluorescence detection used proliferation. Autophagy protein was...
: The current study aims to explore the phenotype and genotype of a mutation Ala291Thr, which responsible for type I protein C (PC) deficiency in Chinese woman. PROC antigen was tested with chromogenic substrate method. gene were amplified by PCR direct sequencing. Bioinformatics model analysis used harm mutation. PC activity (PC: A) levels three members reduced 39, 57 56%, respectively, PC: decreased parallelly same as A. Sequencing showed proband novel heterozygous c.997G>A point exon 9...
Congenital coagulation factor VII (FVII) deficiency is a rare disorder caused by mutation in F7 gene. Herein, we reported patient who had unexplained hematuria and vertigo with consanguineous parents. He has been diagnosed as having FVII based on the results of reduced activity (2.0%) antigen (12.8%). The thrombin generation tests verified that proband obstacles producing thrombin. Direct sequencing analysis revealed novel homozygous missense p.Trp284Gly. Also noteworthy fact mutational...
: The objective is to study a gene mutation (Tyr503Cys) found in Chinese consanguineous marriage family with inherited factor XI (FXI) deficiency (cross-reacting material positive, type II). FXI activity and antigen were tested clotting assay ELISA, respectively. was amplified by PCR direct sequencing. ClustalX-2.1-win three online bioinformatics softwares used the conservatism harm of mutation. proband had reduced FXI: at 13%; members decreased about 35%, all whom nomal antigen. DNA...
: To explore the phenotype and genotype of a Chinese family with hereditary factor V deficiency. Routine blood coagulation indexes were detected by one-stage clotting method, whereas antigen was ELISA. All exons intron-exon boundaries F5 gene amplified PCR sequenced directly. The suspected mutation confirmed reverse sequencing. Bioinformatics softwares used to analyze possible impact this mutation. Phenotypic analysis showed that proband had significantly prolonged prothrombin time activated...
Both compound heterozygous and homozygous protein C deficiencies (PCDs) can cause lethal thrombotic events in children. This study investigated the significance of F139V mutation activation biallelic PCD. Two pedigrees with three probands were recruited, including heterozygous, PCD non-PCD families. The plasma levels activity (PC:A), antigen (PC:Ag), factor V:C, VIII:C, fibrinogen (FIB), D-dimer (D-D) measured. Prothrombin time (PT), activated partial thromboplastin (APTT), thrombin (TT)...
To analyze the causative gene and molecular pathogenesis in a pedigree with compound hereditary coagulation factor V deficiency. Routine blood indexes antigen (FV:Ag) were detected by one-stage clotting method ELISA. Function of mutant protein was evaluated Calibrated Automated Thrombogram (CAT). The amplified PCR direct sequencing. possible impact mutations analyzed bioinformatics tools. proband's activity FV:Ag reduced to 3 6%. Gene sequencing revealed heterozygous c.911G>A (Gly276Glu)...
Objective To identify potential mutations of the FXII gene (F12) in a consanguineous marriage family with hereditary coagulation factor XII (FXII) deficiency, and it will improve understanding pathogenesis involved disease.Clinical presentation The proband was 58-year-old male who had chronic gastritis. He found to have significantly prolonged activated partial thromboplastin time (APTT) at 101.0s (reference range, 29.0–43.0 s) before stomachendoscopy.Techniques activity (FXII:C) antigen...
Congenital hypofibrinogenemia is a rare disorder caused by heterozygous mutations in one of the three fibrinogen genes--fibrinogen α-chain (FGA), β-chain (FGB) and γ-chain (FGG)--which code for Aα, Bβ γ chains, respectively. In this study, we identified genetic defect FGG underlying hypofibrinogenemia. The proposita had prolonged blood clotting time (thrombin 24.5 s, prothrombin 16.8 s) low level plasma (0.71 g/l Clauss method 0.79 immunoturbidimetry). DNA screening whole gene revealed GC...
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To analyze the types of genetic variants and clinical characteristics three Chinese pedigrees affected with Hereditary coagulation factor Ⅶ (FⅦ) deficiency.