A5073389641- Muscle Physiology and Disorders
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Mesenchymal stem cell research
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Exercise and Physiological Responses
- Virus-based gene therapy research
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Parathyroid Disorders and Treatments
- Protease and Inhibitor Mechanisms
- Metabolism and Genetic Disorders
- Silk-based biomaterials and applications
- Autophagy in Disease and Therapy
- Hippo pathway signaling and YAP/TAZ
- Nutrition and Health in Aging
- Laser Applications in Dentistry and Medicine
- RNA modifications and cancer
- Caveolin-1 and cellular processes
- Spaceflight effects on biology
- Cardiomyopathy and Myosin Studies
- Body Contouring and Surgery
Kawasaki Medical School
2017-2023
Kyushu University
2014-2018
RIKEN BioResource Research Center
2012
The aim of this study was to evaluate the efficacy and safety high-dose taurine supplementation for prevention stroke-like episodes MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis episodes), a rare genetic disorder caused by point mutations in mitochondrial DNA that lead modification defect at first anticodon nucleotide tRNALeu(UUR), resulting failure decode codons accurately.After nationwide survey MELAS, we conducted multicentre, open-label, phase III trial which 10 patients...
A recent study demonstrated a positive effect of apple polyphenol (APP) intake on muscle endurance young-adult animals. While an enhancement lipid metabolism may be responsible, in part, for the improvement, contributing mechanisms still need clarification. Here we show that 8-week 5% (w/w) APP diet, up-regulates two features related to fiber type: ratio myosin heavy chain (MyHC) type IIx/IIb and myoglobin protein expression plantaris 9-week-old male Fischer F344 rats compared pair-fed...
Apobec2 is a member of the activation-induced deaminase/apolipoprotein B mRNA editing enzyme catalytic polypeptide cytidine deaminase family expressed in differentiated skeletal and cardiac muscle. We previously reported that deficiency mice leads to shift muscle fiber type, myopathy, diminished mass. However, mechanisms myopathy caused by its physiologic functions are unclear. Here we show that, although localizes sarcomeric Z-lines mouse tissue cultured myotubes, structure not affected...
Abstract Regenerative mechanisms that regulate intramuscular motor innervation. including configuration of the neuromuscular connections are thought to reside in spatiotemporal expression axon‐guidance molecules. Our previous studies proposed a heretofore unexplored role satellite cells as key source secreted neural chemorepellent semaphorin 3A ( Sema3A ) expression. In order verify this concept, there is still critical need provide direct evidence show up‐regulation protein vivo upon muscle...
Abstract Recently, we found that resident myogenic stem satellite cells upregulate a multi-functional secreted protein, semaphorin 3A (Sema3A), exclusively at the early-differentiation phase in response to muscle injury; however, its physiological significance is still unknown. Here show Sema3A impacts slow-twitch fiber generation through signaling pathway, cell-membrane receptor (neuropilin2-plexinA3) → myogenin-myocyte enhancer factor 2D slow myosin heavy chain. This novel axis was by...
Abstract Our previous studies demonstrated that an 8‐week intake of 5% (w/w) apple polyphenol (APP) in the diet improves muscle endurance young‐adult rats. In order to identify a lower limit dietary contribution APP effect, experiments were designed for lower‐dose supplementation (8‐week feeding 0.5% AIN‐93G diet) 12‐week‐old male Sprague‐Dawley Results clearly showed significantly up‐regulates slower myosin‐heavy‐chain (MyHC) isoform ratios (IIx and IIa relative total MyHC) myoglobin...
Abstract Regenerative intramuscular motor‐innervation is thought to reside in the spatiotemporal expression of axon‐guidance molecules. Our previous studies showed that resident myogenic stem cells, satellite up‐regulate a secreted neural‐chemorepellent semaphorin 3A ( S ema3 A ) during early‐differentiation period, response hepatocyte growth factor HGF elevated injured muscle. However, paracrine source release still unknown. Very recently, we proposed possible contribution anti‐inflammatory...
Regenerative mechanisms that regulate intramuscular motor innervation are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed an unexplored role resident myogenic stem cell (satellite cell)-derived myoblasts as a key presenter secreted neural chemorepellent semaphorin 3A (Sema3A); hepatocyte growth factor (HGF) and basic fibroblast (FGF2) triggered its exclusively at early differentiation phase. In order advance this concept, present...
Mutations of the caveolin 3 gene cause autosomal dominant limb-girdle muscular dystrophy (LGMD)1C. In mice, overexpression mutant leads to loss and results in myofiber hypotrophy association with activation neuronal nitric oxide synthase (nNOS) at sarcolemma. Here, we show that directly bound nNOS suppressed its phosphorylation-dependent a specific residue, Ser1412 nicotinamide adenine dinucleotide phosphate (NADPH)-flavin (FAD) module near C-terminus reduction domain, vitro. Constitutively...
This paper provides in vitro phenotypical data to show that APOBEC2, a member of apoB mRNA editing enzyme, catalytic polypeptide-like family, may implicate self-renewal functions myogenic stem satellite cells, namely the re-establishment quiescent status after activation and proliferation myoblasts single-myofiber culture.
The data presented in this article are related to the research articles entitled "APOBEC2 negatively regulates myoblast differentiation muscle regeneration" and "Data supporting possible implication of APOBEC2 self-renewal functions myogenic stem satellite cells: toward understanding negative regulation differentiation" (Ohtsubo et al., 2017a, 2017b) [1,2]. This provides vivo phenotypical show that Paired Box Transcription Factor 7 (Pax7)-positive cell number (per myofiber) is significantly...