Many investigations about the cellular response by metal oxide nanoparticles in vitro have been reported. However, influence of adsorption ability toward cells is unknown. The aim this study to understand on cell viability vitro. abilities six kinds nanoparticles, namely, NiO, ZnO, TiO2, CeO2, SiO2, and Fe2O3, Dulbecco's modified Eagle's medium supplemented with a 10% fetal bovine serum (DMEM-FBS) component such as proteins Ca2+ were estimated. All adsorbed DMEM-FBS; particular, ZnO showed...

Blackcurrants (Ribes nigrum L.) contain high levels of anthocyanin polyphenols, which have beneficial effects on health, owing to their antioxidant and anticarcinogenic properties. Phytoestrogens are plant-derived substances with estrogenic activity, could the skin. Estradiol secretion decreases during menopause, reducing extracellular matrix (ECM) component production by skin fibroblasts. Using a normal human female fibroblast cell line (TIG113) ovariectomized rats, present study...

Scope Blackcurrants (Ribes nigrum L., Grossulariaceae) contain high amounts of anthocyanin polyphenols, which have antioxidant and anti-carcinogenic health benefits. This study analyzed the potential phytoestrogenic effects blackcurrant extract (BCE) in breast cancer (MCF-7) human endometrial (Ishikawa) cell lines that over-express estrogen receptor alpha (ERα), as well immature female rats. Methods results Microarray analysis Ingenuity® Pathway Analysis showed BCE activated ERα pathway,...

Phytoestrogens are plant compounds with estrogenic effects found in many foods. We have previously reported phytoestrogen activity of blackcurrant anthocyanins (cyanidin-3-glucoside, cyanidin-3-rutinoside, delphinidin-3-glucoside, and delphinidin-3-rutinoside) via the estrogen receptor (ER)α. In this study, we investigated participation ERβ these anthocyanins. Blackcurrant anthocyanin induced ERβ-mediated transcriptional activity, IC50 was lower than that ERα, indicating a higher binding...

Exosomes are membrane-derived extracellular vesicles that have recently been recognized as important mediators of intercellular communication. In the present study, we investigated effects exosomes derived from SW480 colorectal cancer cells in recipient HepG2 hepatocellular cells. We demonstrated SW480-derived were taken up by via dynamin-dependent endocytosis and localized to lysosomes. addition, induced phosphorylation signal-regulated kinase (ERK)1/2 following their uptake into Of note,...

Phytoestrogens are plant-derived chemicals that found in many foods and have estrogenic activity. We previously showed blackcurrant extract (BCE) anthocyanins phytoestrogenic activity mediated via estrogen receptors (ERs), may improve vascular function. BCE contains high levels of anthocyanins, but their health-promoting effects unclear. This study examined the on regulation endothelial nitric oxide synthase (eNOS) expression (NO) synthesis human cells as key regulators cardiovascular...

Estrogen is involved in lipid metabolism. Menopausal women with low estrogen secretion usually gain weight and develop steatosis associated abnormal A previous study showed that blackcurrant (Ribes nigrum L.) extract (BCE) had phytoestrogen activity. In this study, we examined whether BCE improved metabolism abnormalities reduced liver ovariectomized rats, as a menopausal animal model. Twelve-week-old (OVX) rats were fed regular diet (Ctrl) or 3% supplemented while sham for three months....

Alopecia is known as a symptom of acute radiation, yet little concerning the mechanism this phenomenon and alteration hair protein profiles. To examine this, 6-week-old male C57/BL6 mice were exposed to 6 Gy X-ray irradiation, which caused alopecia. Their skin collected, proteins analyzed with liquid chromatography/electrospray-ionization mass spectrometry immunohistochemistry. No change was observed in composition major keratins, such Krt81, Krt83 Krt86. However, cytokeratin Krt15 CD34, are...

Ovarian cancer is the most common cause of gynecological malignancy-related mortality since early-stage disease difficult to diagnose. Advanced clear cell carcinoma ovary (CCCO) has dismal prognosis, and its incidence been increasing in Japan, emphasizing need for highly sensitive diagnostic prognostic CCCO biomarkers. Exosomal microRNAs (miRNAs) secreted by tumor cells are known play a role carcinogenesis; however, their involvement ovarian unclear. In this study, we performed expression...

Osteoporosis risk increases in menopausal individuals owing to the decrease estrogen secretion. Blackcurrant extract (BCE) ameliorates osteoporosis; however, underlying mechanisms are unclear. Furthermore, although BCE has phytoestrogenic activity, its effects on osteoblasts unknown. In present study, we investigated BCE-mediated attenuation of osteoporosis using mouse MC3T3-E1 pre-osteoblasts, with a focus osteogenesis. After treating cells for 48 h, cell proliferation was assessed Cell...

Most models of hereditary hypotrichosis are due to alterations in growth factors and transcription factors, the examples causative mutations hair keratin genes limited. The Hirosaki hairless rat (HHR) is a mutant strain spontaneously derived from Sprague-Dawley rats (SDRs). In this study, locus responsible gene was examined by linkage analysis mapped on chromosome 7q36. Because many basic clustered 7q36, their expression examined. Reverse transcription-PCR genomic PCR indicated that Kb21...

Background: Blackcurrant anthocyanin (BCA) is expected to repair endothelial dysfunction, but it remains unclear whether beneficial effects are present in young healthy persons. This study examines supplements containing blackcurrant improve function and peripheral temperature smokers. Methods: Young, male nonsmokers (N group: n = 11; mean age 22 ± 2 years) smokers (S 13; 21 1 were enrolled. A randomized double-blind trial was designed compare the of no supplement, a supplement 50 mg...

Blackcurrants (Ribes nigrum L., Grossulariaceae) possess a high content of anthocyanin polyphenols, which have been demonstrated to exhibit beneficial effects on health due their antioxidant and anticarcinogenic prope-rties. The present study investigated novel functions anthocyanin‑rich blackcurrant extracts (BCEs) in healthy mammary epithelial cell line, MCF10A. percentages viable cells were 85, 75, 53 31% following exposure 50, 100, 200 400 µg/ml BCE, respectively. half‑maximal response...

Kuromoji (Lindera umbellata) is a tree that grows throughout Japan. The components of kuromoji essential oil have antitumor and aromatherapy effects. However, the composition hydrosol, obtained as by-product process, unknown. Furthermore, it unknown whether has deodorizing effect. Therefore, purpose current study was to compare chemical well evaluate effect former. samples evaluated using gas chromatography–mass spectrometry (GC-MS). Additionally, investigated with detector tube method...

Alzheimer's disease (AD) is associated with a progressive worsening in cognitive function, which often accompanied by emotional disturbance. Recent studies revealed that anthocyanin-rich blackcurrant extract (BCE) can impart health benefits, but it not known whether BCE useful the prevention and/or treatment of AD. Here, we examined effects using senescence-accelerated mouse prone 8 (SAMP8) model. Dietary supplementation for 9 weeks was found to both improve diminished long-term recognition...

Ancocyanin-rich blackcurrant extract (BCE) has phytoestrogen activity; however, its effect on hair follicles is unknown. Additionally, loss known to occur during menopause in women owing decreased estrogen secretion. This study examined whether BCE alleviated female pattern using a rat model. RNA was extracted and analyzed microarray ingenuity pathway analysis. A quantitative polymerase chain reaction revealed that 1 μg/mL altered many genes downstream of beta-estradiol human dermal papilla...

OHK cells, a human lymphoma cell line, are known to produce large amounts of hyaluronan. We investigated the effect 4-methylumbelliferone, an inhibitor hyaluronan synthesis, on activity matrix metalloproteinases in cells. Matrix metalloproteinase-9 was detected gelatin zymography as main metalloproteinase excreted into medium cultured and 4-methylumbelliferone added decreased enzyme dose-dependent manner. Addition Streptomyces hyaluronidase during cultivation did not decrease activity....