A5101477047- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Electrochemical Analysis and Applications
- Aquaculture Nutrition and Growth
- Meat and Animal Product Quality
- Peroxisome Proliferator-Activated Receptors
- Aquaculture disease management and microbiota
- Electrochemical sensors and biosensors
- Proteins in Food Systems
- Adipose Tissue and Metabolism
- Microbial Natural Products and Biosynthesis
- Protein Hydrolysis and Bioactive Peptides
- Vibrio bacteria research studies
- Molecular Sensors and Ion Detection
- Food composition and properties
- Microbial Metabolic Engineering and Bioproduction
- Nanoplatforms for cancer theranostics
- Advanced Sensor and Energy Harvesting Materials
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Adipokines, Inflammation, and Metabolic Diseases
- Genomics and Phylogenetic Studies
- Cholesterol and Lipid Metabolism
- interferon and immune responses
- Conducting polymers and applications
Ocean University of China
2020-2025
Ningbo University
2012-2025
Ministry of Agriculture and Rural Affairs
2020-2025
Harbin Institute of Technology
2024-2025
Yunnan Agricultural University
2024
Jiangsu Academy of Agricultural Sciences
2022-2024
West Anhui University
2024
China Electronics Technology Group Corporation
2024
Institute of Microbiology
2015-2023
Chinese Academy of Sciences
2015-2023
A clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a-mediated dual-mode electrochemical biosensor without polymerase chain reaction (PCR) amplification was designed for sensitive and reliable detection of genetically modified soybean SHZD32-1. functionalized composite bionanomaterial Fe3O4@AuNPs/DNA-Fc&Ru synthesized as the signal unit, while a characteristic gene fragment SHZD32-1 chosen target DNA (tDNA). When Cas12a, crRNA, tDNA were present simultaneously, ternary...
An electrochemiluminescence (ECL) inhibition method combined with molecularly imprinted solid phase extraction (MISPE) was developed for quantitative determination of malachite green (MG) residues in fish. It found that MG could strongly inhibit the ECL signal luminol. Under optimized conditions, quenched intensity versus logarithm concentration good linear relationship over a range from 20 to 5000 ppt. The detection limit be about 6 Molecularly polymers (MIPs) were synthesized as (SPE)...
Abstract Streptococcus mutans is a major pathogen causing human dental caries. As Gram-positive bacterium with small genome (about 2 Mb) it considered poor source of natural products. Due to recent explosion in genomic data available for S. strains, we were motivated explore the product production potential this organism. Bioinformatic characterization 169 publically genomes from caries revealed surprisingly rich biosynthetic gene clusters. Anti-SMASH analysis identified one nonribosomal...
Resibufogenin (RB) has been used for cancer treatment, but the underlying mechanisms are still unclear. This study aimed to investigate effects of RB treatment on colorectal (CRC) cells, and determine mechanisms. The cell counting kit-8 assay was viability. Cell morphology observed under light microscopy, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling employed detect apoptosis. Intracellular ferrous iron (Fe2+ ), malondialdehyde (MDA), glutathione (GSH), reactive...
Co-infection with HIV-1 and Kaposi's sarcoma-associated herpesvirus (KSHV) is the cause of aggressive AIDS-related sarcoma (AIDS-KS) characterized by abnormal angiogenesis. The impact KSHV interaction on pathogenesis extensive angiogenesis AIDS-KS remains unclear. Here, we explored synergistic effect Tat oncogene Orf-K1 Our results showed that soluble or ectopic expression enhanced K1-induced cell proliferation, microtubule formation in chorioallantoic membrane nude mice models. Mechanistic...
Anaerobic bacteria represent an overlooked rich source of biological and chemical diversity. Due to the challenge cultivation genetic intractability, assessing capability their biosynthetic gene clusters (BGCs) for secondary metabolite production requires efficient heterologous expression system. However, this kind host system is still unavailable. Here, we use facultative anaerobe Streptococcus mutans UA159 as a BGCs from anaerobic bacteria. A natural competence based large DNA fragment...
Intestinal barrier disruption due to the intestinal epithelial cells' (IECs) death is one of critical pathological features inflammatory bowel diseases (IBDs). SM934, an artemisinin analog, has previously been proven ameliorate colitis induced by dextran sulfate sodium (DSS) in mice suppressing inflammation response. In this study, we investigated protective effects SM934 on and underlying mechanism trinitrobenzene sulfonic acid (TNBS)-induced mice. We demonstrated that restored body weight...
In the 21 st century, intestinal homeostatic imbalance has emerged as a growing health challenge worldwide. Accumulating evidence reveals that excessive intake of saturated fatty acid (SFA) induces imbalance. However, potential molecular mechanism is still unclear. present study, we found palm oil or palmitic (PA) treatment disturbed lipid metabolism homeostasis and triggered endoplasmic reticulum (ER) stress inflammation in intestine cells large yellow croaker ( Larimichthys crocea )....
Liver cancer is a deadly malignancy associated with high mortality and morbidity. Less than 20% of patients advanced liver respond to single anti-PD-1 treatment. The heterogeneity neutrophils in the tumor immune microenvironment may contribute resistance checkpoint blockade (ICB). However, underlying mechanism remains largely unknown.We established an orthotopic model by using transposable elements integrate oncogenes Myc KrasG12D into genome cells from conditional Trp53 null/null mice...