A5100356451- Antibiotic Resistance in Bacteria
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Pneumonia and Respiratory Infections
- interferon and immune responses
- Pharmacological Effects of Natural Compounds
- Bacterial Genetics and Biotechnology
- Veterinary medicine and infectious diseases
- Liver physiology and pathology
- COVID-19 and Mental Health
- Amoebic Infections and Treatments
- Parasites and Host Interactions
- Hepatitis B Virus Studies
- Phytochemistry and biological activity of medicinal plants
- Organ Transplantation Techniques and Outcomes
- Infections and bacterial resistance
- COVID-19 epidemiological studies
- Microbial Community Ecology and Physiology
- Oral microbiology and periodontitis research
- Helicobacter pylori-related gastroenterology studies
- Healthcare professionals’ stress and burnout
Hubei University of Medicine
2016-2025
Wuhan Institute of Virology
2024-2025
Chinese Academy of Sciences
2024-2025
Guangdong Ocean University
2025
Hangzhou First People's Hospital
2024
Westlake University
2024
Taihe Hospital
2011-2022
Fujian Medical University
2020-2022
Southern Medical University
2020-2022
Wuhan University
2020-2022
Abstract Background More than 210,000 medical workers have fought against the outbreak of Coronavirus Disease 2019 (COVID-19) in Hubei China since December 2019. However, prevalence mental health problems frontline staff after fighting COVID-19 is still unknown. Methods Medical Wuhan and other cities Province were invited to participate a cross-sectional convenience sampling online survey, which assessed anxiety, insomnia, depression, post-traumatic stress disorder (PTSD). Results A total...
Effective treatments for acute-on-chronic liver failure (ACLF) are lacking. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have been applied in tissue regeneration and repair, acting through paracrine effects, cell fusion, actual transdifferentiation. The present study was designed to investigate the therapeutic potential of hUC-MSCs injury (ACLI) ACLF rat models.Wistar rats aged 6 weeks were intraperitoneally administered porcine serum (PS) at a dose 0.5 mL twice per week 11...
Corona Virus Disease 2019 (COVID-19) is a highly prevalent and potent infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Until now, the world still endeavoring to develop new ways diagnose treat COVID-19. At present, clinical prevention treatment of COVID-19 mainly targets spike protein on surface SRAS-CoV-2. However, with continuous emergence SARS-CoV-2 Variants concern (VOC), targeting therapy shows high degree limitation. The Nucleocapsid Protein (N...
Schistosomiasis, also called bilharziasis, is a neglected tropical disease induced by Schistosoma spp. that causes hundreds of millions infections. Although ova-induced granulomas commonly cause inflammation, hyperplasia, ulceration, microabscess formation, and polyposis, the role egg granuloma on gut microbiome remains unclear. To explore role, microbial communities in mice infected with japonicum were surveyed. Female C57BL/6 BALB/c exposed to cercariae S. for 45 days 65 then sacrificed....
ABSTRACT The nucleocapsid protein of SARS-CoV-2 plays significant roles in viral assembly, immune evasion, and stability. Due to its immunogenicity, high expression levels during COVID-19, conservation across strains, it represents an attractive target for antiviral treatment. In this study, we identified characterized a single-stranded DNA aptamer, N-Apt17, which effectively disrupts the liquid-liquid phase separation (LLPS) mediated by N protein. To enhance aptamer’s stability, circular...
Comorbidities, such as obesity, increase the risk of severe COVID-19. However, mechanisms underlying illnesses in individuals with obesity are poorly understood. Here, we used gene-edited leptin knock out (Leptin -/-) obese hamsters to establish a infection model. This model exhibits robust viral replication, lung lesions, pronounced clinical symptoms, and fatal infection, mirroring COVID-19 patients obesity. Using single-cell transcriptomics on tissues pre- post-infection, found that...
The mammalian intestine is a major site of colonization and starting point severe infections by Klebsiella pneumoniae. Inflammatory bowel disease (IBD) an inflammatory disorder the gut, host-derived nitrate in IBD confers luminal growth advantage upon Escherichia coli Salmonella typhimurium through respiration inflamed gut. However, impact on pathogenicity K. pneumoniae this microenvironment poorly understood. In study, we used oral administration dextran sodium sulfate to induce mouse...
Streptococcus suis is an important zoonotic pathogen that threatens human and pig health. During infection, the host can impose oxidative stress to resist invasion. Resistance toxicity factor for pathogens. Glutathione synthesis contributes reactive oxygen species (ROS) detoxification in bacterial cells. Little known about roles of glutathione transport S. suis. In this study, we demonstrated treatment increased tolerance GshAB GshT were found import by bioinformatics. vitro, inactivation...
The global regulator cAMP receptor protein (CRP) has been shown to be required for the full virulence and/or expression of determinants in a wide set bacterial pathogens. In this work, crp mutant as well complemented was constructed from wild-type Klebsiella pneumoniae capsular serotype K1 strain causing primary pyogenic liver abscess. phenotypes strain, and were characterized systematically. It disclosed that K. CRP vitro growth, fimbria production, biofilm formation lethality mouse, but it...
To investigate the early epidemic of COVID-19, a total 176 confirmed COVID-19 cases in Shiyan city, Hubei province, China were surveyed. Our data indicated that province outside Wuhan emerged by migrate rate-dependent, and second-generation patients mainly cluster-onset between family members from travelers. Epidemiological investigation 10.7% transmitted asymptomatic SARS-CoV-2 infected individuals. Moreover, among cases, 53 admitted to Renmin Hospital University Medicine. The clinical...
Chronic gastric infection by the Gram-negative bacterium Helicobacter pylori (H. pylori) is strongly associated with gastritis, ulcer and development of distal carcinoma mucosal lymphoma in humans. Antibiotic treatment H. becoming less effective because increasing antibiotic resistance; other approaches such as specifically targeted methods, etc. to destroy this organism would be beneficial. An epitope vaccine a promising option for protection against infection. In study, multi-epitope was...
Abstract With the advantages of wide distribution, fast growth, and broad metabolic spectrum to organic carbon compounds, Vibrio may play an important role in cycling. However, ecological roles many marine environments have not been explored. Here, world’s deepest ‘blue hole’, Sansha Yongle Blue Hole (SYBH) South China Sea, which is a geographically semi-enclosed environment featuring unique chemical characters, was investigated. The abundance, diversity source utilization capability were...
ABSTRACT Plague is one of the most dangerous diseases and caused by Yersinia pestis . Effective vaccine development requires understanding immune protective mechanisms against bacterium in humans. In this study, humoral memory cellular responses plague patients ( n = 65) recovered from Y. infection during past 16 years were investigated using a protein microarray an enzyme-linked immunosorbent spot assay (ELISpot). The seroprevalence to F1 antigen all 78.5%. infected more than decade ago,...
Vibrio spp. are ubiquitous marine bacteria with high metabolism flexibility and genome plasticity. Previous studies have revealed the dynamics of planktonic vibrios in relation to environmental forces, such as temperature salinity. However, little is known about ecology benthic environments. Here, we elucidate abundance, diversity, spatial distribution sediments Chinese marginal seas, a wide range from north south covering Yellow Sea (YS), East China (ECS), South (SCS). Quantitative analysis...
This study was performed to test the association between Helicobacter pylori (HP) and periodontal disease (PD).This a case-control in comprehensive hospital, including all patients with newly diagnosed PD 2012 2014 as cases without controls, thorough examinations. Those who tested positive for HP were examined by means of polymerase chain reaction. Single multivariate logistic regression used analyze data using SPSS 19.0 software.This included 212 Han Chinese non-smoking adults. The results...
Klebsiella pneumoniae is an opportunistic pathogen, and its hypervirulent variants cause serious invasive community-acquired infections. A genomic view of K. NTUH-2044 for the carbohydrate phosphotransferase system (PTS) found a putative fructose PTS, namely, Frw PTS gene cluster. The deletion mutant complemented frwC (KP1_1992), which encodes fructose-specific enzyme IIC, were constructed, phenotypes characterized. This transmembrane protein responsible utilization. can enhance biofilm...
Klebsiella pneumoniae is a pathogen known for its high frequency of antimicrobial resistance. Responses to various environmental stresses during life can influence the resistance antibiotics. Here, we demonstrate role and mechanism KbvR regulator in response osmotic stress effect on The kbvR mutant strain exhibited increasing tolerance certain antibiotics, including β-lactams. expression levels outer membrane porin OmpK36 were upregulated wild type (WT), deletion decreased level ompK36....
Bacteria can modify their morphology in response to environmental stimuli for survival or host defense evasion. The rich glucose vivo the Luria-Bertani (LB) medium shortened cell length of K. pneumoniae. decreased levels cyclic AMP (cAMP) and transcription crp, which declined cAMP-CRP activity. crp deletion mutant was significantly shorter than that wild type (0.981± 0.057 μm vs. 2.415±0.075 μm, P<0.001). These results indicated high alters bacterial a round form through regulating activity...
Biofilm formation is important in both the environmental and intestinal phases of Vibrio cholerae life cycle. Nevertheless, most studies V. biofilm focus on monospecies cultures, whereas nearly all communities found nature consist a variety microorganisms. Multispecies biofilms formed between other bacteria environment interactions that exist these species are still poorly understood. In this study, influence Escherichia coli was studied context vitro coculture vivo coinfection. To...
Severe COVID-19 patients exhibit impaired IFN-I response due to decreased IFN-β production, allowing persistent viral load and exacerbated inflammation. While the SARS-CoV-2 nucleocapsid (N) protein has been implicated in inhibiting innate immunity by interfering with signaling, specific underlying mechanism still needs further investigation for a comprehensive understanding. This study reveals that N enhances interaction between human SUMO-conjugating enzyme UBC9 MAVS. Increased MAVS-UBC9...
ABSTRACT Klebsiella pneumoniae is an important opportunistic pathogen that causes a variety of infections. It critical for bacteria to maintain metal homeostasis during infection. By using isogenic mntP deletion mutant K. strain NTUH-K2044, we found MntP was manganese efflux pump. Manganese increased the tolerance oxidative stress, and stress could increase intracellular concentration. In exhibited significantly higher sensitivity manganese. Furthermore, iron Inductively coupled plasma mass...
Klebsiella pneumoniae is an opportunistic pathogen that mostly affects patients with weakened immune systems, but a few serotypes (especially K1 and K2) are highly invasive result in systemic infection healthy persons. The ability to evade survive the components of innate system critical infection. To investigate role mechanism transcription regulator KP1_RS12260 (KbvR) virulence defense against response, kbvR deletion mutant complement strains were constructed. vivo animal assay vitro...