A5079728082- Cell Adhesion Molecules Research
- Medical Imaging Techniques and Applications
- Medical and Biological Sciences
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Immunotherapy and Immune Responses
- Mycorrhizal Fungi and Plant Interactions
- Cellular Mechanics and Interactions
- Genomics and Phylogenetic Studies
- Glaucoma and retinal disorders
- Virus-based gene therapy research
- RNA Research and Splicing
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Protease and Inhibitor Mechanisms
- Molecular Biology Techniques and Applications
- Insect symbiosis and bacterial influences
- Plant Disease Resistance and Genetics
- Alzheimer's disease research and treatments
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Retinal Development and Disorders
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Fermentation and Sensory Analysis
RIKEN Center for Integrative Medical Sciences
2011-2024
Osaka University
2008-2011
Kellogg's (Canada)
2011
Aichi Medical University
2002-2008
Japan Science and Technology Agency
2005-2008
Protein Research Foundation
2008
University of Virginia
2002
Osaka Medical Center for Cancer and Cardiovascular Diseases
1997-1999
Hokkaido University
1990-1994
GIT1 is a multidomain protein that thought to function as an integrator of signaling pathways controlling vesicle trafficking, adhesion and cytoskeletal organization. It regulates ARF GTPases has binding domains for paxillin PIX, which PAK-binding exchange factor Rac. We show cycles between at least three distinct subcellular compartments, including adhesion-like structures, the leading edge cytoplasmic complexes. The also contain paxillin, PAK do not detectably co-localize with endosomal...
Fibronectin (FN) has a complex pattern of alternative splicing at the mRNA level. One alternatively spliced segments, EDA, is prominently expressed during biological processes involving substantial cell migration and proliferation, such as embryonic development, malignant transformation, wound healing. To examine function EDA segment, we overexpressed recombinant FN isoforms with or without in CHO cells compared their cell-adhesive activities using purified proteins. EDA+ was significantly...
Extracellular matrix (ECM), which provides critical scaffolds for all adhesive cells, regulates proliferation, differentiation, and apoptosis. Different cell types employ customized ECMs, are thought to play important roles in the generation of so-called niches that contribute cell-specific functions. The molecular entities these however, have not been elucidated. Here, we describe a strategy transcriptome-wide identification ECM proteins based on computational screening >60,000 full-length...
Abstract Autotrophic eukaryotes have evolved by the endosymbiotic uptake of photosynthetic organisms. Interestingly, many algae and plants secondarily lost activity despite its great advantages. Prototheca Helicosporidium are non-photosynthetic green possessing colourless plastids. The plastid genomes wickerhamii sp. highly reduced owing to elimination genes related photosynthesis. To gain further insight into reductive genome evolution during shift from a heterotrophic lifestyle, we...
Integrin alpha8beta1 interacts with a variety of Arg-Gly-Asp (RGD)-containing ligands in the extracellular matrix. Here, we examined binding activities integrin toward panel RGD-containing ligands. bound specifically to nephronectin an apparent dissociation constant 0.28 +/- 0.01 nm, but showed only marginal affinities for fibronectin and other The high-affinity was fully reproduced recombinant fragment derived from central "linker" segment. A series deletion mutants identified LFEIFEIER...
Summary Alzheimer’s disease (AD) is a major cause of dementia, with the number patients this condition anticipated to exceed 50 million worldwide in near future. Despite extensive research efforts, no effective measures are available facilitate prevention or treatment AD, which due part lack animal models able closely replicate human-like state. Here, we describe generation three mutant marmoset individuals exon 9 PSEN1 gene product has been deleted ( -ΔE9). Such ΔE9 mutations have reported...
Polyploidization events such as whole-genome duplication and inter-species hybridization are major evolutionary forces that shape genomes. Although long-term effects of polyploidization have been well-characterized, early molecular consequences remain largely unexplored. Here, we report the discovery two recent independent genome hybridizations within a single clade fungal genus, Trichosporon Comparative genomic analyses revealed redundant genes experiencing decelerations, not accelerations,...
We screened more than 60000 RIKEN mouse cDNAs for novel ECM (extracellular matrix) proteins by extensive computational screening followed recombinant expression and immunohistochemical characterization. identified two olfactomedin-family characterized the presence of tandem CXCXCX9C motifs in N-terminal region, a coiled-coil domain an olfactomedin C-terminal region. These proteins, named photomedin-1 photomedin-2, were secreted as disulphide-bonded dimers (photomedin-1) or...
Nonhuman primates (NHPs), which are closely related to humans, useful in biomedical research, and an increasing number of NHP disease models have been reported using gene editing. However, many disease-related genes cause perinatal death when manipulated homozygously by In addition, resources, limited, should be efficiently used. Here, address these issues, we developed a method introducing heterozygous genetic modifications into common marmosets combining Platinum transcription...
Abstract To construct a backbone tree consisting of basidiomycetous yeasts, draft genome sequences from 25 species Trichosporonales (Tremellomycetes, Basidiomycota) were generated. In addition to the hybrid genomes Trichosporon coremiiforme and ovoides that we described previously, identified an interspecies in Cutaneotrichosporon mucoides (formerly ). This had gene retention rate ~55%, its closest haploid relative was dermatis . After constructing C subgenomes, generated phylogenetic using...
Cerebral small vessel disease (CSVD) causes dementia and gait disturbance due to arteriopathy. autosomal recessive arteriopathy with subcortical infarcts leukoencephalopathy (CARASIL) is a hereditary form of CSVD caused by loss high-temperature requirement A1 (HTRA1) serine protease activity. In CARASIL, intimal thickening, smooth muscle cell (SMC) degeneration, elastic lamina splitting, vasodilation. The molecular mechanisms were proposed involve the accumulation matrisome proteins as...
The order Trichosporonales (Tremellomycotina, Basidiomycota) includes various species that have clinical, agricultural and biotechnological value. Thus, understanding why how evolutionary diversification occurred within this is extremely important. This study clarified the phylogenetic relationships among Tricosporonales species. To select genes suitable for analysis, we determined draft genomes of 17 extracted 30 protein-coding DNA sequences (CDSs) from genomic data. CDS regions...
In the fission yeast, two Tor isoforms, Tor1 and Tor2, oppositely regulate gene expression of amino acid permeases. To elucidate transcriptional machinery for these regulations, here we have employed cap analysis (CAGE), a method analyzing profiles identifying start sites (TSSs). The loss decreased, Tor2 inhibition by its temperature sensitive mutation increased, mRNA isp5+, per1+, put4+ SPBPB2B2.01. contrast, cat1+. These changes were confirmed semi-quantitative RT-PCR. opposite effects...
ABSTRACT The Japanese oak wilt pathogen Raffaelea quercivora and the platypodid beetle, Platypus quercivorus , cause serious mass mortality of Quercus spp. in Japan. Here, we present first draft genome sequence R. JCM 11526 to increase our understanding mechanism pathogenicity symbiosis with ambrosia beetle.
Abstract Genome hybridization is an important evolutionary event that gives rise to species with novel capabilities. However, the merging of distinct genomes also brings together incompatible regulatory networks must be resolved during course evolution. Understanding early stages post-hybridization evolution particularly because changes in these have long-term consequences. Here, via comparative transcriptomic analyses two closely related, recently hybridized Trichosporon fungi, T ....
FN and Integrin Beta 1 colocalize by fluorescence microscopy ( View interaction ) Paxillin
Abstract We propose ‘integrated synthetic genetics’ as a novel methodology that integrates reductive and approaches used in life science research. Integrated genetics enables determinations of sets genes required for the functioning any biological subsystem. This method utilizes artificial cell-like compartments, including randomly introduced whole gene library, strictly defined components vitro transcription translation reporter fluoresces ‘only when particular function target subsystem is...
Motivation The T cell receptors are expressed as millions of different rearrangements. Amplified a complex mixture PCR products, they can be sequenced directly on next-generation instruments without the need for cloning. This method is increasingly used to characterize, quantify and study these highly diverse receptors. Results We present here clonotypeR, software package identify analyze antigen from high-throughput sequence libraries. ClonotypeR designed process, organize very large...