Oxidative stress (OS) is recognized as a contributing factor in the development and progression of thyroid cancer. Nuclear erythroid 2-related 2 (Nrf2) pivotal transcription involved against OS generated by excessive reactive oxygen species (ROS). It governs expression wide array genes implicated detoxification antioxidant pathways. However, studies have demonstrated that sustained activation Nrf2 can contribute to tumor drug resistance cancers. The was notably elevated papillary cancer...

Heart failure is a common systemic disease with high morbidity and mortality worldwide. Doxorubicin (DOX) commonly used anthracycline broad-spectrum antitumor antibiotic strong effect definite curative effect. However, cardiotoxicity the adverse reaction of drug dose cumulative toxicity, but mechanism still unclear. In this study, proteomics metabonomics techniques were to analyze tissue plasma DOX-induced heart (HF) in rats clarify molecular harmful effects DOX on cardiac metabolism...

Silibinin, as the major active constituent of silymarin, has its various biological effects. Here, we investigated inhibitory effects silibinin on HeLa cell growth in relation to autophagy and apoptosis induced by reactive oxygen species (ROS) nitrogen (RNS) generation. Silibinin dose time-dependently decreased cultured medium containing 10% fetal bovine serum or free media (SFM) with an IC(50) approximately 80-100 40-60 μM at 24 h, respectively. 12 confirmed monodansylcadervarine (MDC)...

Silibinin is an active constituent extracted from blessed milk thistle (Silybum marianum). Our previous study demonstrated that silibinin induced autophagy and apoptosis via reactive oxygen species (ROS) generation in HeLa cells. In this study, we investigated whether the autophagy- apoptosis-associated molecules also involved ROS generation. promoted expression phosphorylated-p53 (p-p53) a dose-dependent manner. Pifithrin-α (PFT-α), specific inhibitor of p53, reduced production reversed...

Avocados are one of the important fruits in our diet, showing many health benefits. However, a significant amount avocados become defective as they transported throughout supply chain and refused by consumers, ending up at animal or pet feed manufacturers. Indeed, some previous evidence suggests that rejected still present high phenolic content can be reused drug pharmacological industry. Therefore, work, we measured from avocado pulp evaluated antioxidant potential, followed...

Triptolide (TP), the main active ingredient of Tripterygium wilfordii Hook F., has great potential in treatment autoimmune diseases. However, it been found that side effects TP involve multiple organs and systems, which most serious relate to kidney. The mechanism nephrotoxicity caused by requires further investigation. In present study, we integrated proteomic metabolomic methods identify proteins small molecule metabolites associated with TP-induced nephrotoxicity. There was a significant...

Increasing research has concentrated on the anti-tumour efficacy of silibinin, a flavonolignan that is clinically used as an hepatoprotectant. However, previous work found silibinin-induced apoptosis accompanied by protective superoxide (O(2)*-) generation in MCF-7 cells. This study further reports formation reactive nitrogen species (RNS) same system. It finds silibinin induces nitric oxide (*NO) time- and concentration-dependent manner. Moreover, results support there exists...

We previously demonstrated that oridonin-induced autophagy enhanced efferocytosis (phagocytosis of apoptotic cells) by macrophage-like U937 cells through activation the inflammatory pathways. In this study, exposure to 2.5 μM oridonin caused up-regulation inducible nitric oxide synthase (iNOS) expression and continuous endogenous generation (NO), which was reversed pre-treatment with inhibitors 1400 W (dihydrochloride) or L-NAME (hydrochloride). NO donor sodium nitroprusside (SNP) irritant...

Silibinin is an active constituent extracted from the blessed milk thistle (Silybum marianum). In a previous study, we demonstrated that silibinin treatment induced generation of reactive nitrogen species (RNS), which were associated with oxygen (ROS), and caused apoptosis autophagy in HeLa cells. Another study reported attenuated apoptotic effect sodium nitroprusside (SNP) by generating ROS rat pheochromocytoma PC12 cells []. To clarify relationship between RNS nitric oxide (NO) cells,...

Oridonin, a diterpenoid compound, extracted and purified from Rabdosia rubescen has been reported to have cytotoxic effect on tumour cells through apoptosis, tyrosine kinase pathways are involved in these processes. A specific epidermal growth factor receptor (EGFR) inhibitor AG1478 was used examine the relationship between EGFR signal oridonin-induced apoptosis autophagy abundant human epidermoid carcinoma A431 cells. Inhibition of EGFRaugmented cell while changes expression downstream...

Metabonomics was used to find characteristics of nephrotoxicity induced by IP or IV injection cisplatin.

Cor pulmonale is characterized by severe right ventricular dysfunction caused lung disease, particularly chronic obstructive pulmonary which can lead to hypertension. Our previous study has demonstrated that Fuzi and Beimu compatibility (FBC), a traditional TCM taboo, improves function in early-stage of hypertension through the synergistic action β-ARs signals. However, FBC increases cardiotoxicity with prolonged treatment disease progression. Considering environment influences exertion...

The interaction between small-molecule compounds of traditional Chinese medicine and their direct targets is the molecular initiation event, which key factor for toxicity efficacy. Psoralen, an active component Fructus Psoraleae, toxic to liver has various pharmacological properties. Although mechanism psoralen-induced hepatotoxicity been studied, target psoralen remains unclear. Thus, aim this study was discover psoralen. To end, we initially used proteomics based on drug affinity...

The interaction of small molecules with direct targets constitutes the molecular initiation events drug efficacy and toxicity. Aconitine, an active compound Aconitum species, has various pharmacological effects but is strongly toxic to heart. aconitine-induced cardiotoxicity remain unclear.We predicted aconitine based on network pharmacology followed a novel proteomic approach "drug affinity responsive target stability" technology combined LC-MS/MS identify aconitine. identified were...