A5063096108- Metabolism and Genetic Disorders
- Brucella: diagnosis, epidemiology, treatment
- Mitochondrial Function and Pathology
- Virology and Viral Diseases
- Animal Virus Infections Studies
- Diet and metabolism studies
- Folate and B Vitamins Research
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Burkholderia infections and melioidosis
- RNA modifications and cancer
- Lysosomal Storage Disorders Research
- interferon and immune responses
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Plant Pathogenic Bacteria Studies
- Mosquito-borne diseases and control
Washington University in St. Louis
2023-2025
Institute of Special Animal and Plant Sciences
2011-2023
China Agricultural University
2016-2021
Chinese Academy of Agricultural Sciences
2013-2021
Henan Normal University
2021
Jilin Academy of Agricultural Sciences
2020
Northeast Agricultural University
2017
Dezhou University
2007-2015
Kunming Medical University
2015
Northwest A&F University
2013
Paramyxoviruses are significant human and animal pathogens that include mumps virus (MuV), Newcastle disease (NDV) the murine parainfluenza Sendai (SeV). Despite their importance, few host factors implicated in paramyxovirus infection known. Using a recombinant SeV expressing destabilized eGFP (rSeVC dseGFP ) loss-of-function CRISPR screen, we identified CMP-sialic acid transporter (CST) gene SLC35A1 UDP-galactose (UGT) SLC35A2 as essential for infection. As expected, knockout (KO) cells...
Copyback viral genomes (cbVGs) are truncated with complementary ends produced when the negative-sense RNA virus polymerase detaches from replication template and resumes elongation nascent strand. Despite advances in methods to identify cbVGs based on site of break rejoin, PCR-based tools cannot provide full length sequences most and/or can introduce errors artifacts during cbVG amplification. These limitations have painted a limited picture diverse population generated infection. To improve...
We previously demonstrated that W proteins from different Newcastle disease virus (NDV) strains localize in either the cytoplasm (e.g., NDV strain SG10) or nucleus La Sota). To clarify mechanism behind these cell localization differences, we overexpressed protein derived four associated with cellular regions Vero cells. This revealed key region for determining is 180-227aa. Further experiments found there a nuclear export signal (NES) motif 211-224aa. could be transported into via...
Summary Respiratory viruses including the human parainfluenza (hPIVs) are a constant burden to health, with morbidity and mortality frequently increased after acute phase of infection. Although is proven that respiratory can persist in vitro , mechanisms virus or viral products persistence, their sources, impact on chronic diseases vivo unknown. Here, we used Sendai (SeV) model hPIV infection mice test whether persistence associates development lung disease. Following SeV infection, were...
ABSTRACT The henipaviruses, including Nipah virus (NiV) and Hendra (HeV), are biosafety level 4 (BSL-4) zoonotic pathogens that cause severe neurological respiratory disease in humans. To study the replication machinery of these viruses, we developed robust minigenome systems can be safely used BSL-2 conditions. nucleocapsid (N), phosphoprotein (P), large protein (L) henipaviruses critical elements their thus essential support components systems. Here, tested effects diverse combinations...
Due to drawbacks of live attenuated vaccines, much attention has been focused on screening Brucella-protective antigens as subunit vaccine candidates. Here, an immunoproteomic assay was used identify the immunogenic soluble proteins Brucella melitensis 16M. In present study, 27 unique were identified from two-dimensional electrophoresis immunoblot profiles by liquid chromatography tandem MS (LC-MS/MS). From this set, gene encoding one immunodominant protein interest,...
To investigate the exact effects of different origins Newcastle disease virus (NDV) hemagglutinin-neuraminidase (HN) protein to biological characteristics virus, we systematically studied correlation between HN and NDV virulence by exchanging velogenic or lentogenic strains with from other virulence. The results revealed that rSG10 rLaSota derivatives bearing gene viruses exhibited decreased increased hemadsorption (HAd), neuraminidase fusion promotion activities. In vitro in vivo tests...
‘Granny Smith’ apples growing under normal sunlight develop green skin, whereas the peel turns red due to anthocyanin accumulation after removal of a bagging treatment. Two anthocyanins, Cyanidin 3-O-galactoside (cy3-gal) and 3-O-arabinoside (cy3-ara), were detected in apple peels, cy3-gal was determined be chiefly responsible for color. The content more than 98% total peels. To better understand molecular basis biosynthesis apples, we performed quantitative real-time PCR (qRT-PCR) analysis...
Antiviral responses are often accompanied by translation inhibition and formation of stress granules (SGs) in infected cells. However, the triggers for these processes their role during infection remain subjects active investigation. Copy-back viral genomes (cbVGs) primary inducers mitochondrial antiviral signaling (MAVS) pathway immunity Sendai virus (SeV) respiratory syncytial (RSV) infections. The relationship between cbVGs cellular infections is unknown. Here, we show that SGs form...
Reticuloendotheliosis virus (REV) causes an immunosuppressive, oncogenic, and runting syndrome in many avian hosts worldwide. The immunosuppression caused by REV can decrease the antibody levels of vaccines subsequent increase susceptibility to secondary infections. There are no commercial or effective drugs control infections at present. To investigate infection status chickens China, 25,224 sera from 573 chicken flocks, collected between 2005 2015, were measured for antibodies using...
Brucella Cu-Zn superoxide dismutase (Cu-Zn SOD) is a periplasmic protein, and immunization of mice with recombinant SOD protein confers protection against abortus infection. However, the role during process infection remains unknown. Here, we report that secreted into culture medium translocated host cells independent type IV secretion systems (T4SS). Furthermore, co-immunoprecipitation immunofluorescence studies reveal interacts small GTPase Sar1. Overexpression in inhibits bacterial...
Summary Introduction Glucose‐6‐phosphate dehydrogenase (G6 PD ) deficiency is the most common human enzyme defect caused by G6 gene mutations. This study aimed to develop a cost‐effective, multiplex, genotyping method for detecting mutations in gene. Methods We used SN aPshot approach genotype multiple that are populations South‐East Asia. assay based on multiplex PCR coupled with primer extension reactions. Different were determined peak retention time and colors of products. Results...