A5001864217- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Epilepsy research and treatment
- Photoreceptor and optogenetics research
- Pharmacological Effects and Toxicity Studies
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Genomics and Rare Diseases
- EEG and Brain-Computer Interfaces
- Cellular transport and secretion
- Sleep and Wakefulness Research
- Global Health and Surgery
- Ion channel regulation and function
- Bacterial Infections and Vaccines
- Neuroinflammation and Neurodegeneration Mechanisms
- Axon Guidance and Neuronal Signaling
- Infectious Encephalopathies and Encephalitis
- Neuroscience and Neural Engineering
Humboldt-Universität zu Berlin
1997-2022
Charité - Universitätsmedizin Berlin
2010-2022
Freie Universität Berlin
2022
Caucasus University
2020
Institut de Neurophysiopathologie
2011
State University of New York
2005
Boston University
2005
Heidelberg University
2005
University Hospital Heidelberg
2005
University of Leeds
2004
As a structure involved in learning and memory, the hippocampus functions as network. The functional differentiation along longitudinal axis of is poorly demarcated comparison with transverse axis. Using patch clamp recordings conjunction post hoc anatomy, we have examined pattern connectivity long hippocampus. Here, provide anatomical physiological evidence that prominent rhythmic network activities hippocampus, behavior-specific gamma theta oscillations, are seen predominantly axes...
Using whole-cell patch-clamp recordings in conjunction with post hoc anatomy we investigated the physiological properties of hippocampal stratum oriens and pyramidale inhibitory interneurones, before following induction pharmacologically evoked gamma frequency network oscillations. Prior to kainate-induced transient epochs activity, two distinct classes lacunosum-moleculare (O-LM) trilaminar cells, showed prominent differences their membrane firing properties, as well amplitude kinetics...
Gamma frequency (30–80 Hz) network oscillations have been observed in the hippocampus during several behavioral paradigms which they are often modulated by a theta (4–12 oscillation. Interneurons of shown to be crucially involved rhythms generation, and subtypes with distinct anatomy physiology described. In particular, oriens lacunosum-moleculare (O-LM) interneurons were synapse on distal apical dendrites pyramidal cells spike preferentially at frequency, even presence gamma-field...
Controlling the Axon The cellular mechanisms and circuits involved in gamma oscillations brain are not fully understood. Dugladze et al. (p. 1458 ) simultaneously performed patch-clamp recordings soma axon of hippocampal pyramidal neurons during slices. Under these conditions, cells were divided into two electrogenic compartments: fired at low frequency, whereas, axon, ectopic action potentials generated higher frequencies. This functional separation was maintained by highly active axoaxonic...
Mesial temporal lobe epilepsy (mTLE) is one of the most common forms epilepsy, characterized by hippocampal sclerosis and memory deficits. Injection kainic acid (KA) into dorsal hippocampus mice reproduces major electrophysiological histopathological characteristics mTLE. In extracellular recordings from morphologically intact ventral KA-injected epileptic mice, we found that theta-frequency oscillations were abolished, whereas gamma persisted both in vivo vitro. Whole-cell further showed...
The mechanisms that regulate the strength of synaptic transmission and intrinsic neuronal excitability are well characterized; however, promote disease-causing neural network dysfunction poorly defined. We generated mice with targeted neuron type–specific expression a gain-of-function variant neurotransmitter receptor for glycine (GlyR) is found in hippocampectomies from patients temporal lobe epilepsy. In this mouse model, GlyR terminals glutamatergic cells or parvalbumin-positive...
Abstract In patients with drug-resistant epilepsy, surgical resection of the seizure focus may represent best treatment option. Georgia, a structured epilepsy surgery program was lacking until 2017, when German-Georgian project initiated to establish necessary facilities, define diagnostic and standards, educate local healthcare providers. After scientific symposium in Tbilisi six intervention visits have taken place. Georgian epileptologists neuropsychologist visited Berlin-Brandenburg...
Significance Metabotropic GABA B receptors control synaptic transmission and excitability in neuronal circuits of the brain. Although effects these are predominantly inhibitory at both cellular network levels, application agonist baclofen can promote induce seizures patients animal models epilepsy. Here we demonstrate that proepileptic concentration dependent result from disinhibition. high doses, reduces due to its combined pre- postsynaptic pyramidal cells, low it leads an enhanced...
Gamma rhythms are known to contribute the process of memory encoding. However, little is about underlying mechanisms at molecular, cellular and network levels. Using local field potential recording in awake behaving mice concomitant whole-cell recordings slice preparations we found that gamma lead activity-dependent modification hippocampal networks, including alterations sharp wave-ripple complexes. Network plasticity, expressed as long-lasting increases wave-associated synaptic currents,...
Abstract Medial entorhinal cortex (EC) deep layer neurons projecting to the dentate gyrus (DG) were studied. Neurons, retrogradely‐labelled with rhodamine‐dextran‐amine characterized electrophysiologically patch clamp technique and finally labelled biocytin. Pyramidal nonpyramidal form projections from layers of EC molecular DG. In addition, both classes projection send ascending axon collaterals superficial EC. Both physiologically by regular action potential firing upon depolarizing...
Midbrain raphe nuclei provide strong serotonergic projections to the hippocampus, in which serotonin (5-HT) exerts differential effects mediated by multiple 5-HT receptor subtypes. The functional relevance of this diversity information processing is poorly understood. Here we show that via 5-HT<sub>1B</sub> heteroreceptors substantially reduces synaptic excitation cholecystokinin-expressing interneurons area CA1 rat contrast parvalbumin-expressing basket cells. reduction input specific,...
The hippocampal output structure, the subiculum, expresses two major memory relevant network rhythms, sharp wave ripple and gamma frequency oscillations. To this date, it remains unclear how distinct types of subicular principal cells, intrinsically bursting regular spiking neurons, participate in these rhythms. Using concomitant local field potential intracellular recordings an vitro mouse model that allows investigation both we found a cell type-specific segregation neurons into...
The medial entorhinal cortex (mEC) plays a critical role for spatial navigation and memory. While many studies have investigated the principal neurons within cortex, much less is known about inhibitory circuitries this structure. Here, we describe first time in mEC subset of parvalbumin-positive (PV+) interneurons (INs)-stuttering cells (STUT)-with morphological, intrinsic electrophysiological, synaptic properties distinct from fast-spiking PV+ INs. In contrast to INs, axon STUT INs also...
The subiculum (Sub) is the principal target of CA1 pyramidal cells. It serves as final relay hippocampal output and thus mediates hippocampal-cortical interaction. In addition, Sub receives direct input from entorhinal cortex via temporoammonic pathway. this study, we demonstrate that low-frequency stimulation pathway results in disinhibition excitatory synaptic transmission at CA1-Sub synapses. We provide evidence mediated by an NMDA receptor-dependent long-term depression (LTD) GABAergic...