A5049264288- Mechanical Circulatory Support Devices
- HER2/EGFR in Cancer Research
- Cardiac Structural Anomalies and Repair
- Estrogen and related hormone effects
- Breast Cancer Treatment Studies
- Melanoma and MAPK Pathways
- Monoclonal and Polyclonal Antibodies Research
- Cardiac Arrest and Resuscitation
- Protein Kinase Regulation and GTPase Signaling
- Heart Failure Treatment and Management
- Breast Implant and Reconstruction
- Cell death mechanisms and regulation
- Cancer Treatment and Pharmacology
- Cancer-related Molecular Pathways
- Breast Lesions and Carcinomas
- Advanced Breast Cancer Therapies
- Cancer survivorship and care
- Global Cancer Incidence and Screening
- Cytokine Signaling Pathways and Interactions
- BRCA gene mutations in cancer
- Radiomics and Machine Learning in Medical Imaging
- Advanced Biosensing Techniques and Applications
- Colorectal Cancer Treatments and Studies
- Reproductive tract infections research
- Fuel Cells and Related Materials
Freeman Hospital
2018-2024
Newcastle upon Tyne Hospitals NHS Foundation Trust
2020-2024
University of Glasgow
2005-2023
University Hospital Crosshouse
2011-2022
Freeman Health System
2021
Newcastle University
2021
Albany Medical Center Hospital
2021
NHS Ayrshire and Arran
2020
National Health Service Scotland
2020
Ninewells Hospital
2018
Oestrogen receptor (ERalpha) expression is a strong predictor of response to endocrine therapy. The PI3K/AKT/mTOR signal transduction pathway has been implicated in resistance vitro. present study was carried out test the hypothesis that AKT activation mediates tamoxifen clinical breast cancer. Immunohistochemistry (IHC) using AKT1-3, pan-AKT, pAKT (Thr-308), (Ser-473), pER (Ser-167), and pHER2 antibodies performed on 402 ERalpha-positive carcinomas from patients treated with tamoxifen. High...
Aim : Immunohistochemical analysis of protein expression is central to most clinical translational studies and defines patient treatment or selection criteria for novel drugs. Interobserver variation rarely analysed despite recognition that this a key area potential inaccuracy. Therefore our aim was examine observer suggest the revision current standards. Methods results We inter‐ intra‐observer variation, by interclass correlation coefficient (ICCC) κ statistics, in 8661 samples....
Abstract ERBB4/HER4 (referred to here as ERBB4) is a unique member of the epidermal growth factor receptor (EGFR) family tyrosine kinases. In contrast other three members EGFR (i.e., EGFR, ERBB2/HER2/NEU, and ERBB3), which are associated with aggressive forms human cancers, ERBB4 expression seems be selectively lost in tumors phenotypes. Consistent this observation, we show that induces apoptosis when reintroduced into breast cancer cell lines or endogenous activated by ligand. We further...
Resistance to tamoxifen is linked overexpression of HER2, and aromatase inhibitors show particular benefit in progesterone receptor (PR)-negative patients. We previously reported reduced survival patients overexpressing HER1, HER3. now that both HER1-3 PR status predicts for early relapse estrogen (ER)-positive tamoxifen-treated breast cancer patients.Tissue microarray technology was used analyze 402 ER-positive Immunohistochemistry using epidermal growth factor receptor, HER3, HER4,...
We have shown previously that whilst overexpression of HER1, 2 and 3 is associated with poor prognosis in breast cancer, HER4 a good prognosis. Cell proliferation key component aggressive cancers driven by growth factors. In this study bromodeoxyuridine-derived indices are correlated clinical outcome HER1-4 status to further clarify the differing roles for HER family at biological level. 78 invasive had BrdU vivo labelling determine index (BLI) potential tumour doubling time (T pot ). Long...
Abstract Purpose: Amplified in breast cancer 1 (AIB1) is a member of the p160/steroid receptor coactivators family and involved estrogen-dependent gene transcription by reducing antagonistic activity tamoxifen-bound estrogen receptor-α (ER-α). The present study was carried out to test hypothesis that AIB1 protein expression and/or amplification mediates tamoxifen resistance cancer. Experimental Design: Immunohistochemistry using antibody fluorescence situ hybridization probes specific for...
We demonstrate here a new concept termed "oncogene tolerance" whereby human EGF receptor 2 (HER2) increases sphingosine kinase 1 (SK1) expression in estrogen receptor-positive (ER(+)) MCF-7 HER2 cells and SK1, turn, limits negative-feedback manner. The HER2-dependent increase SK1 also p21-activated protein (p65 PAK1) extracellular signal regulated 1/2 (ERK-1/2) signaling. Sphingosine 1-phosphate signaling via S1P(3) is altered cells. In this regard, S1P binding to induces migratory phenotype...
We present a retrospective analysis on cohort of low-grade, node-negative patients showing that human epidermal growth factor receptor 2 (HER2) status significantly affects the survival in this otherwise very good prognostic group. Our results provide support for use adjuvant trastuzumab who are typically classified as having prognosis, not routinely offered standard chemotherapy, and such do fit current UK prescribing guidelines trastuzumab.
In an earlier randomised controlled trial, we showed that early stage breast cancer patients who received a supervised exercise programme, with discussion of behaviour change techniques, had psychological and functional benefits 6 months after the intervention. The purpose this study was to determine if observed at persisted 18 60 later. Women were in original trial contacted Original measures repeated. Of 148 women from agreed be again, 114 attended for follow-up 87 months. intervention...
Abstract Purpose: The expression and activation of the Ras/Raf-1/mitogen-activated protein kinase (MAPK) pathway plays an important role in development progression cancer, may influence response to treatments such as tamoxifen chemotherapy. In this study we investigated whether key components influenced clinical outcome, test hypothesis that MAPK drives resistance chemotherapy women with breast cancer. Experimental Design: Breast tumors from patients at Glasgow Royal Infirmary others treated...
Abstract Background Wire localization is historically the most common method for guiding excision of non-palpable breast lesions, but there are limitations to technique. Newer technologies such as magnetic seeds may allow some these challenges be overcome. The aim was compare safety and effectiveness wire seed techniques. Methods Women undergoing standard or lesions between August 2018 2020 were recruited prospectively this IDEAL stage 2a/2b platform cohort study. primary outcome defined...
Elevated c-Src protein expression has been shown in breast cancer and vitro evidence suggests a role endocrine resistance. To investigate whether is involved resistance, we examined the of both total activated human specimens from cohort oestrogen receptor (ER)-positive tamoxifen-treated patients. Tissue microarray technology was employed to analyse 262 tumour taken before tamoxifen treatment. Immunohistochemistry using antibodies performed. Kaplan-Meier survival curves were constructed...
Aims: CCND1 and EMSY, on 11q13, are frequently amplified in breast cancer. is implicated cell cycle progression EMSY a BRCA2‐associated repressor protein. The aim was to investigate gene copy numbers of determine if amplification associated with reduced survival tamoxifen‐treated cancer patients. Methods results: Fluorescence situ hybridization (FISH) performed 111 consecutive 354 oestrogen receptor (ER)+ cancers. In the set, were 14.8% 7.2%, respectively, deleted 8.7% 13.5%, respectively....
In order to minimise the risk of breast cancer patients for COVID-19 infection related morbidity and mortality prioritisation care has utmost importance since onset pandemic. However, in undergoing surgery not been studied yet. We evaluated safety during pandemic West Scotland region.
We have previously linked HER4 expression with increased survival in breast cancer. However, other reports associated adverse prognostic significance. One possible explanation for the conflicting may be that these results are antibody dependent. The protein is enzymatically cleaved, which alter function of its intracellular domain (ICD). therefore compared staining patterns antibodies against and extracellular domains using tissue microarray technology.Immunohistochemistry was performed...
In a series of 25 patients with suspected pneumonia related to the acquired immune deficiency syndrome (AIDS) first 12 underwent routine fibreoptic bronchoscopy and bronchoalveolar lavage or without transbronchial biopsy before treatment. Eight were found have Pneumocystis carinii had typical clinical presentations prolonged history symptoms, including dry cough, bilateral diffuse alveolar interstitial shadowing in chest radiographs. Among subsequent 13 cases, 11 similar treated high doses...
Tumor necrosis factor α converting enzyme (TACE) mediates shedding of human epidermal growth receptor-4 (HER4). Recent data suggest that released HER4 intracellular domain (4ICD) induces apoptosis in breast cancer. TACE expression, as measured by immunohistochemical analysis, was observed 183 383 carcinomas, 39 217 ovarian and 16 24 17 hormone-sensitive hormone-insensitive prostate respectively. expression detected carcinomas using 2 antibodies recognizing an extracellular or epitope....
Left ventricular assist devices (LVADs) have become an established treatment for advanced heart failure, although with long-term support these patients are potentially exposed to serious complications. Our purpose was assess the role of neutrophil lymphocyte ratio (NLR) in LVAD complications and evaluate if higher values NLR after 4–6 months on (NLR 4_6m) associated worse prognosis. All consecutive who received a HeartWare (N = 188, age 50 ± 13 years), as bridge transplant from December 2009...