A5091436021- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Pharmacogenetics and Drug Metabolism
- Microfluidic and Capillary Electrophoresis Applications
- Biosimilars and Bioanalytical Methods
- Drug Transport and Resistance Mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Analytical Methods in Pharmaceuticals
- Pesticide Residue Analysis and Safety
- Pesticide Exposure and Toxicity
- Analytical chemistry methods development
- Hemoglobinopathies and Related Disorders
- Adipose Tissue and Metabolism
- Cholinesterase and Neurodegenerative Diseases
- Pesticide and Herbicide Environmental Studies
- Metabolism and Genetic Disorders
- Protein purification and stability
- Pharmacological Effects and Assays
- Insect and Pesticide Research
- Nicotinic Acetylcholine Receptors Study
- Phytochemicals and Antioxidant Activities
- Monoclonal and Polyclonal Antibodies Research
- Natural Antidiabetic Agents Studies
- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
Universidade Federal de São Carlos
2014-2024
Bristol-Myers Squibb (United States)
2017
Federal Institute of Education, Science and Technology Alagoas
2016
Indiana University School of Medicine
2015
Indiana University – Purdue University Indianapolis
2015
IQVIA (United States)
2013-2014
Eli Lilly (United States)
2014
National Institute on Aging
2007
Georgetown University
2007
National Institutes of Health
2007
Microwave-assisted sample preparation using diluted nitric acid solutions is an alternative procedure for digesting organic samples. The efficiency of this depends on the chemical properties samples and in work it was evaluated by determination crude protein amount, fat original carbon. Soybeans grains, bovine blood, muscle viscera were digested a cavity-microwave oven oxidant mixtures different concentrations. digestion based residual carbon content element recoveries inductively coupled...
Perturbation of organic anion transporter (OAT) 1- and OAT3-mediated transport can alter the exposure, efficacy, safety drugs. Although there have been reports endogenous biomarkers for OAT1/3, none these all characteristics required a clinical useful biomarker. Cynomolgus monkeys were treated with intravenous probenecid (PROB) at dose 40 mg/kg in this study. As expected, PROB increased area under plasma concentration-time curve (AUC) coadministered furosemide, known substrate OAT1 OAT3, by...
Abstract The aging process causes changes at all organic levels. Although metabolism, cardiac autonomic modulation (CAM), and cardiorespiratory fitness (CRF) are widely studied as a function of age, they mainly in isolation, thus making it difficult to perceive their concomitant variations. This study aimed investigate the integrated that occur metabolome, CAM, CRF throughout apparently healthy individuals. subjects (n = 118) were divided into five groups according age (20–29, 30–39, 40–49,...
SMALL MOLECULES IN BIOLOGICAL MATRICES.Chromatographic methods are commonly used for analysis of small molecules in different biological matrices.An important step to be considered upon a bioanalytical method's development is the capacity yield reliable and reproducible results.This review discusses validation procedures adopted by governmental agencies, such as Food Drug Administration (USA), European Union (EU) Agência Nacional de Vigilância Sanitária (BR) quantification chromatographic...
An integrated automated approach has been developed for the direct determination of drug concentrations using a SCAP DBS system online extraction and analysis dried blood spots (DBS) from paper cards to multidimensional liquid chromatography coupled high-resolution QTOF mass spectrometry (LC-HRMS). accurate, precise, selective, sensitive two-dimensional chromatography-high-resolution (2D LC-HRMS) assay was validated small volumes rat (approximately 1.25 μL) 2 mm punch. The methodology...
Montelukast has been recommended as a selective in vitro and vivo probe of cytochrome P450 (P450) CYP2C8 activity, but its selectivity toward this enzyme remains unclear. We performed detailed characterization montelukast metabolism using human liver microsomes (HLMs), expressed P450s, uridine 5′-diphospho-glucuronosyltransferases (UGTs). Kinetic inhibition experiments at therapeutically relevant concentrations reveal that CYP2C9 are the principal enzymes responsible for 36-hydroxylation to...
Aging process is characterized by a progressive decline of several organic, physiological, and metabolic functions whose precise mechanism remains unclear. Metabolomics allows the identification metabolites may contribute to clarifying aging-regulated pathways. We aimed investigate aging-related serum changes using metabolomics approach. Fasting blood samples from 138 apparently healthy individuals (20−70 years old, 56% men) were analyzed Proton Nuclear Magnetic Resonance spectroscopy (1H...
Abstract Vibrational circular dichroism (VCD) measurements and density functional theory (DFT) calculations were used to obtain the first definitive assignment of absolute configuration for polyphenolic binaphpthyl dialdehyde gossypol a determination solution conformation in CDCl 3 . VCD spectra recorded two resolved enantiomers are near mirror images excellent agreement between observed IR intensity carried out at DFT (B3LYP/6‐31G*) level establish configurations (+)‐gossypol as P...
The development and validation of a HPLC method to follow the degradation phenol by electrochemical or photoelectrochemical treatment are described. This method, which allows determining amounts phenol, p-benzoquinone cathecol in electrolyzed photoelectrolyzed solutions, was used for analysis reaction intermediates during electrooxidation photoelectrooxidation using Ti-Pt/PbO2 (obtained thermal-electrochemical electrodeposition methods) Ti/TiO2 doped with Ga3+ thermal decomposition precursor...
Background: Online automated extraction of dried blood spots (DBS) via direct to a solid-phase (SPE) cartridge and bioanalysis by high-resolution accurate mass spectrometry was examined. The methodology validated used investigate the effect hematocrit on assay bias using partial whole spot extractions from accurately dispensed samples. Results: completed analysis DBS sample accomplished within 2 3 min online DBS-SPE platform. Hematocrit related observed (>15%) for extractions, but not when...
Cytochrome P450 (CYP, P450) presents a wide range of applicability in drug metabolism studies and biocatalysis as great alternative to synthesizing compounds. Nonetheless, their lack stability is one major drawbacks. Aiming the possibility enhancing catalytic activity promoting higher stability, liver microsomal fractions have been immobilized on magnetic beads (Mb). The procedure was modulated by using rat (RLM-Mb). optimal condition achieved further employed for immobilization human...
RATIONALE Pharmacokinetic data to support clinical development of pharmaceuticals are routinely obtained from liquid plasma samples. The samples require frozen shipment and storage extracted off‐line the chromatography/tandem mass spectrometry (LC/MS/MS) systems. In contrast, use dried blood spot (DBS) sampling is an attractive alternative in part due its benefits microsampling as well simpler sample transport. However, a practical aspect, extraction DBS cards can be challenging currently...
In this study, cellular membrane fragments from SH-EP1-pCEP4-hα7 and α7 HEK-293 cell lines were used to synthesize affinity chromatography (CMAC) columns containing functional nicotinic acetylcholine receptors, CMAC(α7 nAChR) columns. The synthesis of stable required the addition cholesterol 2% cholate solubilization/immobilization (s/i) buffer mobile phase. addition, when membranes SH-EP1 line used, l-α-phosphatidylserine l-α-phosphatidylethanolamine also had be added s/i buffer. A...
This work evaluated the potential of isoforms methamidophos to cause organophosphorus-induced delayed neuropathy (OPIDN) in hens. In addition inhibition target esterase (NTE) and acetylcholinesterase (AChE), calpain activation, spinal cord lesions clinical signs were assessed. The (+)-, (±)- (-)-methamidophos administered at 50mg/kg orally; tri-ortho-cresyl phosphate (TOCP) was (500mg/kg, po) as positive control for neuropathy. TOCP hens showed greater than 80% approximately 20% NTE AChE hen...
The current Organisation for Economic Co-operation and Development (OECD) guidelines evaluating organophosphorus-induced delayed neuropathy (OPIDN) require the observation of dosed animals over several days sacrifice 48 hens. Adhering to these protocols in tests with enantiomers is difficult because large quantities compound are needed many must be utilized. Thus, developing an vitro screening protocol evaluate chiral organophosphorus pesticides (OPs) that can induce important. This work...