A5053684848- SARS-CoV-2 and COVID-19 Research
- Infection Control and Ventilation
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Computational Drug Discovery Methods
- COVID-19 and healthcare impacts
- COVID-19 Clinical Research Studies
- Dengue and Mosquito Control Research
- Medical Research and Treatments
- vaccines and immunoinformatics approaches
- Advanced biosensing and bioanalysis techniques
- Zoonotic diseases and public health
- Animal Disease Management and Epidemiology
- Insect-Plant Interactions and Control
- Chemical Synthesis and Analysis
- Influenza Virus Research Studies
- Long-Term Effects of COVID-19
- interferon and immune responses
- Protein Structure and Dynamics
- Malaria Research and Control
- RNA and protein synthesis mechanisms
Uppsala University
2020-2024
COVID-19 is the most rapidly growing pandemic in modern time, and need for serological testing urgent. Although diagnostics of acute patients by RT-PCR both efficient specific, we are also crucially tools investigating antibody responses assessing individual potential herd immunity. We evaluated a commercially available test developed rapid (within 15 minutes) detection SARS-CoV-2-specific IgM IgG 29 PCR-confirmed cases 124 negative controls. The results revealed sensitivity 69% 93.1% IgG,...
Drugs targeting SARS-CoV-2 could have saved millions of lives during the COVID-19 pandemic, and it is now crucial to develop inhibitors coronavirus replication in preparation for future outbreaks. We explored two virtual screening strategies find main protease ultralarge chemical libraries. First, structure-based docking was used screen a diverse library 235 million compounds against active site. One hundred top-ranked were tested binding enzymatic assays. Second, fragment discovered by...
Abstract Evidence suggests that SARS-CoV-2, as well other coronaviruses, can be dispersed and potentially transmitted by aerosols directly or via ventilation systems. We therefore investigated openings in one COVID-19 ward central ducts expel indoor air from three wards at Uppsala University Hospital, Sweden, during April May 2020. Swab samples were taken individual ceiling surfaces ducts. Samples subsequently subjected to rRT-PCR targeting the N E genes of SARS-CoV-2. Central HEPA filters,...
The ongoing SARS-CoV-2 pandemic is a global public health emergency posing high burden on nations' care systems and economies. Despite the great effort put in development of vaccines specific treatments, no prophylaxis or effective therapeutics are currently available. Nitric oxide (NO) broad-spectrum antimicrobial potent vasodilator that has proved to be reducing SARS-CoV replication hypoxia patients with severe acute respiratory syndrome. Given potential NO as treatment for infection, we...
Usutu virus (USUV) is an emerging mosquito-borne flavivirus with increasing prevalence in Europe. Understanding the role of mosquito species USUV transmission crucial for predicting and controlling potential outbreaks. This study aimed to assess vector competence Swedish Culex pipiens USUV. The mosquitoes were orally infected Italian strain (Bologna 2009) infection rates (IR), dissemination (DR), (TR) evaluated over 7 28 days post-infection. revealed that Cx. are susceptible infection, a...
In 2018–2019, Thailand experienced a nationwide spread of chikungunya virus (CHIKV), with approximately 15,000 confirmed cases disease reported. Here, we investigated the evolutionary and molecular history East/Central/South African (ECSA) genotype to determine origins 2018–2019 CHIKV outbreak in Thailand. This was done using newly sequenced clinical samples from travellers returning Sweden late 2018 early 2019 previously published genome sequences. Our phylogeographic analysis showed that...
screening of large compound libraries with automated high-throughput is expensive and time-consuming requires dedicated infrastructures. Conversely, the selection DNA-encoded chemical (DECLs) can be rapidly performed routine equipment available in most laboratories. In this study, we identified novel inhibitors SARS-CoV-2 main protease (M
Abstract Background The ongoing SARS-CoV-2 pandemic has spread rapidly worldwide and disease prevention is more important than ever. In the absence of a vaccine, knowledge transmission routes risk areas infection remain most existing tools to prevent further spread. Methods Here we investigated presence virus in hospital environment at Uppsala University Hospital Infectious Disease ward by RT-qPCR determined infectivity detected vitro on Vero E6 cells. Results RNA was several areas, although...
Abstract Evidence suggests that SARS-CoV-2, as well other coronaviruses, can be dispersed and potentially transmitted by aerosols directly or via ventilation systems. We therefore investigated openings in one COVID-19 ward central ducts expel indoor air from three wards at Uppsala University Hospital, Sweden, during April May 2020. Swab samples were taken individual ceiling surfaces ducts. Samples subsequently subjected to rRT-PCR targeting the N E genes of SARS-CoV-2. Central HEPA filters,...
Japanese encephalitis virus (JEV) is an emerging mosquito-borne Orthoflavivirus that poses a significant public health risk in many temperate and tropical regions Asia. Since the climate some endemic countries similar to climates observed Europe, understanding role of specific mosquito species transmission JEV essential for predicting effectively controlling potential introduction establishment Europe.
Cross-species transmission of influenza A virus (IAV) from wild waterfowl to poultry is the first step in a chain events that can ultimately lead exposure and infection humans. Herein, we study outcome with eight different mallard-origin IAV subtypes two avian hosts: tufted ducks chickens. We found shedding patterns as well innate immune responses were highly dependent on viral subtypes, host species, inoculation routes. For example, intraoesophageal inoculation, commonly used mallard...
Abstract Evidence suggests that SARS-CoV-2 can be dispersed and potentially transmitted by aerosols, directly or via ventilation systems. We report detection of RNA in COVID-19 ward ceiling vent openings as well exhaust filters central ducts up to at least 56 meters from patient areas. As this system provides low air flow, we propose viral particles may readily over vast distances further investigations infectivity airborne must performed.
Infections caused by arthropod-borne RNA viruses are overrepresented among emerging infectious diseases. Effective methods for collecting, storing, and transporting clinical or biological specimens needed worldwide disease surveillance. However, many tropical regions where these diseases endemic lack analytical facilities possibility of continuous cold chains, which presents challenges from both a biosafety material preservation perspective. Whatman® FTA® Classic Cards may serve as an...
Abstract In vitro screening of large compounds libraries with automated high-throughput is expensive, time consuming and requires dedicated infrastructures. Conversely, the selection DNA-encoded chemical (DECL) can be rapidly performed routine equipment available in most laboratories. this study we identified novel inhibitors SARS-CoV-2 main protease (M pro ) through affinity-based DELopen library (open access for academics), containing 4.2 billion compounds. The were peptide-like an...
Abstract In 2018–2019, Thailand experienced a nationwide spread of chikungunya virus (CHIKV), with approximately 15,000 confirmed cases disease reported. Here, we investigated the evolutionary and molecular history East/Central/South African (ECSA) genotype to determine origins 2018–2019 CHIKV outbreak in Thailand. This was done using newly sequenced clinical samples from travellers returning Sweden late 2018 early 2019 previously published genome sequences. Our phylogeographic analysis...
Arboviruses transmitted by mosquitoes, including Japanese encephalitis virus (JEV), present a substantial global health threat. JEV is mosquitoes in the genus Culex, which are common both urban and rural areas Vietnam. In 2020, we conducted 1-year survey of Culex mosquito abundance urban, suburban, peri-urban Hanoi using CDC-light traps. Mosquitoes were identified to species sorted into pools based on species, sex, trap location. The also investigated RT-qPCR for detection JEV. total, 4829...
Abstract We report repeated rRT-PCR detection and infective ability of Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) from a health care worker three days prior to mild COVID-19 symptoms. propose that this provides evidence for potential early presymptomatic transmission SARS-CoV-2 contagiousness may arise shortly after exposure.
Abstract Reports of possible pre- or asymptomatic transmission have been reported, both from SARS-CoV and MERS-CoV outbreaks, although this appears to be uncommon. In contrast, during the COVID-19 pandemic, an increasing number studies case reports indicate that SARS-CoV-2 is not only but also occurs frequently. We report repeated rRT-PCR detection in a health care worker demonstrate infective ability up three days prior mild symptoms. indicated high viral levels approximately after...