A5079057845- SARS-CoV-2 and COVID-19 Research
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- Natural product bioactivities and synthesis
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Probiotics and Fermented Foods
- Toxin Mechanisms and Immunotoxins
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Transgenic Plants and Applications
- Polysaccharides and Plant Cell Walls
- Multiple Sclerosis Research Studies
- Microbial Inactivation Methods
- Veterinary medicine and infectious diseases
- Antibiotic Resistance in Bacteria
- Plant biochemistry and biosynthesis
- Clostridium difficile and Clostridium perfringens research
- Nanocomposite Films for Food Packaging
- Phytochemical Studies and Bioactivities
- Gut microbiota and health
Uppsala University Hospital
2016-2024
Uppsala University
1985-2024
Swedish Veterinary Agency
1995-1997
Swedish University of Agricultural Sciences
1983-1995
United States Army Medical Research Institute of Infectious Diseases
1989
United States Department of the Army
1989
Integrated Cardio Metabolic Centre
1988
Karolinska Institutet
1983
COVID-19 is the most rapidly growing pandemic in modern time, and need for serological testing urgent. Although diagnostics of acute patients by RT-PCR both efficient specific, we are also crucially tools investigating antibody responses assessing individual potential herd immunity. We evaluated a commercially available test developed rapid (within 15 minutes) detection SARS-CoV-2-specific IgM IgG 29 PCR-confirmed cases 124 negative controls. The results revealed sensitivity 69% 93.1% IgG,...
Background In Sweden, information on seroprevalence of tick-borne encephalitis virus (TBEV) in the population, including vaccination coverage and infection, is scattered. This largely due to absence a national (TBE) registry, scarcity previous serological studies use methods not distinguishing between antibodies induced by infection. Furthermore, number notified TBE cases Sweden has continued increase recent years despite increased vaccination. Aim The aim was estimate TBEV Sweden. Methods...
Tick-borne encephalitis virus (TBEV) is an important European vaccine-preventable pathogen. Discrimination of vaccine-induced antibodies from those elicited by infection important. We studied anti-TBEV IgM/IgG responses, including avidity and neutralisation, multiplex serology in 50 TBEV patients vaccinees. Infection induced reactive to both whole (WV) non-structural protein 1 (NS1) 48 clinical cases, whereas 47 vaccinees had WV, but not NS1 antibodies, enabling efficient discrimination...
Colonies of Escherichia coli from blood agar plates were suspended and lysed in saline containing polymyxin B a detergent (Triton X-100). The lysates assayed for heat-labile enterotoxin (LT) by coagglutination test (coa-test). coa-test reagent consisted Formalin-treated heat-killed cells Staphylococcus aureus, strain Cowan 1, sensitized with high-titer rabbit anti-LT serum. Purified LT was detected the at nanogram level (2 to 5 ng), whereas larger amounts cholera toxin (50 ng) required give...
We report a new tool for improved serological diagnostics in suspected tick-borne encephalitis (TBE) vaccine failure cases. Due to an increase the incidence of disease as well number vaccinees, specific and simplified diagnostic methods are needed. Antibody responses TBE-virus (TBEV) non-structural protein 1 (NS1) detectable post TBEV infection but not vaccination. have used samples from 14 previously confirmed Swedish patients study antibody against NS1 whole virus antigens, respectively....
The amino acid sequence of a heat‐stable enterotoxin produced by human strain enterotoxinogenic Escherichia coli was determined to be (disregarding the sulfide bridges): Asn‐Thr‐Phe‐Tyr‐Cys‐Cys‐Glu‐Leu‐Cys‐Cys‐Asn‐Pro‐Ala‐Cys‐Ala‐Gly‐Cys‐Tyr Compensated exchanges have occurred in positions 11 and 18 relation structure from different
Susceptibility to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections is highly variable and could be mediated by a cross-protective pre-immunity. We identified 14 cross-reactive peptides between SARS-CoV-2 influenza A H1N1, H3N2, human herpesvirus (HHV)-6A/B with potential relevance. The H1N1 peptide NGVEGF was identical in the most critical receptor binding motif spike protein that interacts angiotensin converting enzyme receptor. About 62%–73% of COVID-19-negative...
The recent spread of Zika virus (ZIKV) in the Americas and Asia necessitates an increased preparedness for improved maternal perinatal health blood safety. However, serological cross-reactions, especially to Dengue (DENV), complicate ZIKV antibody serodiagnosis. A novel "pan-Flavi" suspension multiplex immunoassay (PFSMIA) using 25 antigens, whole (WV), non-structural protein 1 (NS1), envelope (E) proteins, from 7 zoonotic flaviviruses specific detection DENV IgM IgG was developed. Patterns...
Antibody responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in serum and cerebrospinal fluid (CSF) samples from 16 patients with disease 2019 neurological symptoms were assessed using independent methods. Immunoglobulin G (IgG) specific for the virus spike protein was found 81% of 56% CSF. SARS-CoV-2 IgG CSF observed negative serological findings. Levels both associated severity (P < .05). All elevated markers central nervous system damage also had antibodies = .002),...
Antibodies to microbes, or autoantigens, are important markers of disease. Antibody detection (serology) can reveal both past and recent infections. There is a great need for development rational ways detecting quantifying antibodies, humans animals. Traditionally, serology using synthetic antigens covers linear epitopes up 30 amino acid peptides.We here report that peptides 100 acids longer ("megapeptides"), designed synthesized optimal serological performance, successfully be used as in...
Due to the current, rapidly increasing Coronavirus disease 2019 (COVID-19) pandemic, efficient and highly specific diagnostic methods are needed. The receptor-binding part of spike (S) protein, S1, has been suggested be virus-specific; it does not cross-react with antibodies against other coronaviruses. Three recombinant partial S proteins severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) expressed in mammalian or baculovirus-insect cells were evaluated as antigens a Luminex-based...
The gene for Escherichia coli heat-stable enterotoxin type II (STII) was fused to the genes protein A from Staphylococcus aureus and beta-galactosidase in two different expression systems. Antibodies raised rabbits against A-STII fusion recognized beta-galactosidase-STII protein. latter used as immobilized antigen an enzyme-linked immunosorbent assay (ELISA) detection of STII. correlation between results ELISA intestinal loop test piglets 95%, suggesting that can be reliably detect
Abstract Background Children develop symptomatic coronavirus disease 2019 (COVID‐19) more rarely than adults upon infection with severe acute respiratory syndrome 2 (SARS‐CoV‐2). Pediatric oncology and hematology patients may be at increased risk of COVID‐19 due to their underlying or treatment. We investigated seroprevalence anti‐SARS‐CoV‐2 antibodies, respectively, in a Swedish cohort pediatric patients. Procedure Patients ( n = 136) were recruited between June 2020 September 2021 Uppsala...
Abstract Background Susceptibility to SARS-CoV-2 infections is highly variable, ranging from asymptomatic and mild in most, deadly outcome few. This individual difference susceptibility could be mediated by a cross protective pre-immunity, but the nature of this pre-immunity has remained elusive. Methods Antibody epitope sequence similarities cross-reactive T cell peptides were searched for between other pathogens. We established an ELISA test, Luminex Multiplex bead array assay test...
Introduction: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome. Materials methods: 'Megapeptides', 119–120-mers from the amino terminus of nucleoprotein 16 hantaviruses, representing four major branches hantavirus phylogenetic tree, were utilized in a novel IgG-based suspension multiplex immunoassay (HSMIA) for detection past infections 155 North European human samples. We compared...
The potential of rapid point-of-care (POC) tests has been subject doubt due to an eventual risk production errors. aim was therefore evaluate the two separate lots a commercial POC lateral flow test, intended for detection IgM and IgG against SARS-CoV-2 spike protein (S1). Control samples consisted serum from individuals with confirmed infection pre-COVID-19 negative sera gathered biobank. presence anti-S1 IgM/IgG in verified by in-house Luminex-based serological assay (COVID-19 SIA). One...