A5038217788- Advanced Biosensing Techniques and Applications
- Gene expression and cancer classification
- Veterinary Equine Medical Research
- Monoclonal and Polyclonal Antibodies Research
- Ruminant Nutrition and Digestive Physiology
- Advanced Proteomics Techniques and Applications
- Reproductive Physiology in Livestock
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Genetic Syndromes and Imprinting
- Streptococcal Infections and Treatments
- Adrenal Hormones and Disorders
- Adipose Tissue and Metabolism
- Melanoma and MAPK Pathways
- PI3K/AKT/mTOR signaling in cancer
- HER2/EGFR in Cancer Research
- Protein Kinase Regulation and GTPase Signaling
- Advanced Breast Cancer Therapies
- Biotin and Related Studies
- Neonatal and Maternal Infections
- Oral and Maxillofacial Pathology
- Skin Protection and Aging
- Connexins and lens biology
- Cancer Treatment and Pharmacology
- Ubiquitin and proteasome pathways
Boehringer Ingelheim (Germany)
2022-2024
German Cancer Research Center
2010-2022
Heidelberg University
2010-2022
Institute of Science and Technology Austria
2020-2021
Friedrich Schiller University Jena
2015
Seoul National University Hospital
2012
Instituto Nacional do Câncer
2012
University of Göttingen
2010
Ludwig-Maximilians-Universität München
2010
Centrum für Integrierte Onkologie
1943
Mosaic analysis with double markers (MADM) offers one approach to visualize and concomitantly manipulate genetically defined cells in mice single-cell resolution. MADM applications include the of lineage, morphology physiology, genomic imprinting phenotypes, dissection cell-autonomous gene functions vivo health disease. Yet, can only be applied <25% all mouse genes on select chromosomes date. To overcome this limitation, we generate transgenic knocked-in cassettes near centromeres 19...
Aberrant activation of Hedgehog (HH) signaling has been identified as a key etiologic factor in many human malignancies. Signal strength, target gene specificity, and oncogenic activity HH depend profoundly on interactions with other pathways, such epidermal growth receptor-mediated signaling, which shown to cooperate HH/GLI basal cell carcinoma pancreatic cancer. Our experimental data demonstrated that the Daoy medulloblastoma line possesses fully inducible endogenous pathway. Treatment...
The reverse phase protein array (RPPA) approach was employed for a quantitative analysis of 71 cancer-relevant proteins and phosphoproteins in 84 non-small cell lung cancer (NSCLC) lines by monitoring the activation state selected receptor tyrosine kinases, PI3K/AKT MEK/ERK1/2 signaling, cycle control, apoptosis, DNA damage. Additional information on NSCLC such as that transcriptomic data, genomic aberrations, drug sensitivity analyzed context proteomic data using supervised non-supervised...
Despite promising progress in targeted breast cancer therapy, drug resistance remains challenging. The monoclonal antibody drugs trastuzumab and pertuzumab as well the small molecule inhibitor erlotinib were designed to prevent ErbB-2 ErbB-1 receptor induced deregulated protein signalling, contributing tumour progression. oncogenic potential of ErbB receptors unfolds case overexpression or mutations. Dimerisation with other allows bypass pathway blockades. Our intention is reconstruct...
Analysis of large-scale proteomic data sets requires specialized software tools, tailored toward the requirements individual approaches. Here we introduce an extension open-source solution for analyzing reverse phase protein array (RPPA) data. The R package RPPanalyzer was designed preprocessing followed by basic statistical analyses and visualization. In this update, merged relevant steps into a single user-friendly function included new method background noise correction as well methods...
A robust subclassification of luminal breast cancer, the most common molecular subtype human is crucial for therapy decisions. While a part patients at higher risk recurrence and requires chemo-endocrine treatment, other lower also poorly responds to chemotherapeutic regimens. To approximate cancer recurrence, clinical guidelines recommend determining histologic grading abundance cell proliferation marker in tumor specimens. However, this approach assigns an intermediate substantial number...
Protein responses to extracellular cues are governed by gene transcription, mRNA degradation and translation, protein degradation. In order understand how these time-dependent processes cooperate generate dynamic responses, we analyzed the response of human mammary cells epidermal growth factor (EGF). Integrating transcript data into a mathematical model, inferred for several proteins their pre-and post-stimulus translation coefficients found that they exhibit complex, variation....
Abstract Background A glycemic challenge test is used for the diagnosis of insulin dysregulation (ID) in horses and ponies. Different forms exist where administrative route dose glucose vary, which makes interpretation results challenging. Hypothesis/Objectives To evaluate palatability of, blood responses to, carbohydrate pellets fed as an oral (OGT), to establish diagnostic threshold ID when using pellets. Animals University privately‐owned ponies ( n = 157) comprised 31 breeds both sexes....
High concentrations of adrenocorticotropic hormone (ACTH) are used as an indicator pituitary pars intermedia dysfunction (PPID), but other factors that may influence ACTH need to be understood, if diagnostic reference ranges for with confidence. Insulin dysregulation (ID) could one such factor, insulin affects hormones in species.To test the hypothesis a relationship exists between high and aged (>15-year-old) animals no clinical signs PPID.A cohort study.Thirteen horses eleven ponies (17-25...
Testing for insulin dysregulation (ID) in horses is commonly performed to guide management and therapeutic strategies.
SUMMARY Mosaic Analysis with Double Markers (MADM) offers a unique approach to visualize and concomitantly manipulate genetically-defined cells in mice single-cell resolution. MADM applications include the analysis of lineage; morphology physiology; genomic imprinting phenotypes; dissection cell-autonomous gene functions vivo health disease. Yet, could only be applied <25% all mouse genes on select chromosomes thus far. To overcome this limitation, we generated transgenic knocked-in...
Oral glycemic challenge (GC) tests are recommended for diagnosis of insulin dysregulation (ID). Various protocols used, but all have limitations in terms palatability, ease use, variable composition, geographic availability, or some combination these.To evaluate newly developed formulations with defined carbohydrate composition use as oral GCs.Thirty-four horses and ponies various metabolic states.Our objectives were carried out 2 separate cross-over experiments. First, the palatability...
Targeted therapies have shown striking success in the treatment of cancer over last years. However, their specific effects on an individual tumor appear to be varying and difficult predict. Using integrative modeling approach that combines mechanistic regression modeling, we gained insights into response mechanisms breast cells due different ligand–drug combinations. The multi-pathway model, capturing ERBB receptor signaling as well downstream MAPK PI3K pathways was calibrated time-resolved...
Abstract In this study, the athermal effects of water‐filtered infrared A ( wIRA )‐irradiation (780–1400 nm) on human dermal fibroblasts were investigated. For purpose, cells exposed to ‐irradiation (178 mW cm −2 for 1 h), while a sophisticated experimental setup prevented warming samples exceeding 0.1°C. The investigated parameters formation reactive oxygen species (ROS), mitochondrial membrane potential and superoxide release, protein oxidation, proliferation rate, as well intracellular Ca...
Abstract Summary: Protein microarrays are well-established as sensitive tools for proteomics. Particularly, the microspot immunoassay (MIA) platform enables a quantitative analysis of (phospho-) proteins in complex solutions (e.g. cell lysates or blood plasma) and with low consumption samples reagents. Despite numerous biological clinical applications MIAs there is currently no user-friendly open source data software available versatile options visualization. Here, we introduce...
Mosaic Analysis with Double Markers (MADM) offers a unique approach to visualize and concomitantly manipulate genetically-defined cells in mice single-cell resolution. MADM applications include the analysis of lineage; morphology physiology; genomic imprinting phenotypes; dissection cell-autonomous gene functions vivo health disease. Yet, could only be applied <25% all mouse genes on select chromosomes thus far. To overcome this limitation, we generated transgenic knocked-in cassettes near...