A5072664186- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- COVID-19 Clinical Research Studies
- Cancer, Lipids, and Metabolism
- Neuroblastoma Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- Lung Cancer Treatments and Mutations
- Immune Cell Function and Interaction
- Metabolomics and Mass Spectrometry Studies
- Endoplasmic Reticulum Stress and Disease
- Phagocytosis and Immune Regulation
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Protein Degradation and Inhibitors
- Chromatin Remodeling and Cancer
- Genetic Associations and Epidemiology
- Gene expression and cancer classification
- Psychosomatic Disorders and Their Treatments
- Autophagy in Disease and Therapy
- Single-cell and spatial transcriptomics
- Explainable Artificial Intelligence (XAI)
- Microbial Metabolic Engineering and Bioproduction
- Cancer-related Molecular Pathways
- Phytochemicals and Medicinal Plants
- Drug-Induced Hepatotoxicity and Protection
Heidelberg University
2013-2024
University Hospital Heidelberg
2020-2024
Heidelberg University
2024
German Cancer Research Center
2013-2021
Hopp Children's Cancer Center Heidelberg
2020
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2016
Jena University Hospital
2013
Exacerbated pro-inflammatory immune response contributes to COVID-19 pathology. However, despite the mounting evidence about SARS-CoV-2 infecting human gut, little is known antiviral programs triggered in this organ. To address gap, we performed single-cell transcriptomics of SARS-CoV-2-infected intestinal organoids. We identified a subpopulation enterocytes as prime target and, interestingly, found lack positive correlation between susceptibility infection and expression ACE2. Infected...
The reverse phase protein array (RPPA) approach was employed for a quantitative analysis of 71 cancer-relevant proteins and phosphoproteins in 84 non-small cell lung cancer (NSCLC) lines by monitoring the activation state selected receptor tyrosine kinases, PI3K/AKT MEK/ERK1/2 signaling, cycle control, apoptosis, DNA damage. Additional information on NSCLC such as that transcriptomic data, genomic aberrations, drug sensitivity analyzed context proteomic data using supervised non-supervised...
Advancements in the field of Artificial Intelligence has been rapid recent years and revolutionized various industries. Various deep neural network architectures capable handling both text images, covering code generation from natural language, producing machine translation summarizations have proposed. For example, Convolutional Neural Networks or CNNs perform image classification at a level equivalent to that humans on many datasets. These state-of-the-art networks reached unprecedented...
Research Article17 February 2015Open Access Source Data A high-throughput RNAi screen for detection of immune-checkpoint molecules that mediate tumor resistance to cytotoxic T lymphocytes Nisit Khandelwal Corresponding Author Division Translational Immunology, German Cancer Center (DKFZ), Heidelberg, Germany Search more papers by this author Marco Breinig Signaling and Functional Genomics, Department Cell Molecular Biology, Faculty Medicine Mannheim, Heidelberg University, Tobias Speck...
Endoplasmic reticulum (ER) plays an essential role in cell function and survival. Accumulation of unfolded or misfolded proteins the lumen ER activates protein response (UPR), resulting stress subsequent apoptosis. The alkylphosphocholine erufosine is a known Akt-mTOR inhibitor oral squamous carcinoma (OSCC). In present study, we evaluate erufosine's to induce mitochondrial leading autophagy, apoptosis, ROS induction. cellular toxicity was determined two OSCC lines gene expression enrichment...
Somatic copy number alterations frequently occur in the cancer genome affecting not only oncogenic or tumor suppressive genes, but also passenger and potential codriver genes. An intrinsic feature resulting from such genomic perturbations is deregulation metabolism of cells. In this study, we have shown that metabolic cancer-causing genes are unexpectedly often proximally positioned chromosome share loci with coaltered numbers across multiple cancers (19 types The Cancer Genome Atlas). We...
The TCGA database was analyzed to identify deregulation of cell cycle genes across 24 cancer types and ensuing effects on patient survival. Pan-cancer analysis showed that head neck squamous carcinoma (HNSCC) ranks amongst the top four cancers showing deregulated genes. Also, median gene expression all CDKs cyclins in HNSCC samples higher than global expression. This verified by IHC staining CCND1 from patients. When evaluating quartiles with highest lowest expression, increased CCND1/CDK6...
COVID-19 outbreak is the biggest threat to human health in recent history. Currently, there are over 1.5 million related deaths and 75 people infected around world (as of 22/12/2020). The identification virulence factors which determine disease susceptibility severity different cell types remains an essential challenge. serine protease TMPRSS2 has been shown be important for S protein priming viral entry, however, little known about its regulation. SPINT2 a member family Kunitz type...
The migrational propensity of neuroblastoma is affected by cell identity, but the mechanisms behind divergence remain unknown. Using RNAi and time-lapse imaging, we show that ADRN-type NB cells exhibit RAC1- kalirin-dependent nucleokinetic (NUC) migration relies on several integral components neuronal migration. Inhibition NUC RAC1 kalirin-GEF1 inhibitors occurs without hampering proliferation ADRN identity. three clinically relevant expression dichotomies, reveal most up-regulated mRNAs in...
Unsupervised deconvolution algorithms are often used to estimate cell composition from bulk tissue samples. However, applying cell-type and interpreting the results remain a challenge, even more without prior training in bioinformatics. Here, we propose tool for estimating identifying type transcriptomes or methylomes. DECOMICS is shiny-web application dedicated unsupervised approaches of omic data. It provides (i) variety existing perform on gene expression methylation-level matrix, (ii) an...
Interferon lambdas (IFN-λs) are crucial to control virus infections at mucosal surfaces. Interleukin-22 (IL-22) was reported help IFN-λ rotavirus infection in the intestinal epithelium of mice either by aiding induction interferon-stimulated genes (ISGs) or increasing cell proliferation thereby clearing virally infected cells. We investigated whether IL-22 and IFN-λs exhibit similar synergistic effects human epithelial cells (IECs) models. Our results showed that co-treatment induced more...
Abstract Endoplasmic reticulum (ER) plays an essential role in cell function and survival. Accumulation of unfolded or misfolded proteins the lumen ER activates protein response (UPR), resulting ER-stress subsequent apoptosis. The alkylphosphocholine erufosine is a known Akt-mTOR inhibitor oral squamous carcinoma (OSCC). In present study we decipher new facet erufosine's mechanism action evaluate its to induce mitochondrial stress leading autophagy, apoptosis ROS induction. cellular toxicity...
Ayurveda has its own role in the prevention and management of cancer’s complications. Chemo- radiotherapy are highly toxic both damage adjacent healthy cells. Their side effects may be acute like nausea, vomiting, diarrhea fatigue or chronic pharyngitis, esophagitis, laryngitis persistent dysphasia. According to Ayurveda, many medicines show anticancer properties such as Brihamarasayan, Brahmi, Guduchi, Yasthimadhu, Ashwgandha, Amalaki which can helpful treating cancer well caused by chemo-...
Abstract Head and neck cancer ranks amongst the sixth most prevalent cancers worldwide oral squamous cell carcinomas (OSCC) constitute 90% thereof. Erufosine is an ether-lipid-derived synthetic compound belonging to alkylphosphocholines (APCs), which has been shown inhibit proliferation of OSCC cells. It simultaneously induces apoptosis autophagy by modulating Akt-mTOR signaling pathway, however, its exact mechanism action not fully understood. Here, we describe activity erufosine on...
Abstract COVID-19 outbreak is the biggest threat to human health in recent history. Currently, there are over 1.5 million related deaths and 75 people infected around world (as of 22/12/2020). The identification virulence factors which determine disease susceptibility severity different cell types remains an essential challenge. serine protease TMPRSS2 has been shown be important for S protein priming viral entry, however, little known about its regulation. SPINT2 a member family Kunitz type...