A5030585836- Genetic factors in colorectal cancer
- TGF-β signaling in diseases
- Cancer Cells and Metastasis
- Digestive system and related health
- Colorectal Cancer Treatments and Studies
- HER2/EGFR in Cancer Research
- Cytokine Signaling Pathways and Interactions
- Cancer Research and Treatments
- Gastric Cancer Management and Outcomes
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Treatments and Mutations
- Histone Deacetylase Inhibitors Research
- Ovarian cancer diagnosis and treatment
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Lung Cancer Research Studies
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Glioma Diagnosis and Treatment
- Bone and Dental Protein Studies
- FOXO transcription factor regulation
- Immunotherapy and Immune Responses
Heidelberg University
2017-2022
German Cancer Research Center
2018-2022
DKFZ-ZMBH Alliance
2021
Institut Català d'Oncologia
2009-2016
Institut d'Investigació Biomédica de Bellvitge
2009-2016
Universitat Autònoma de Barcelona
2011-2014
Bellvitge University Hospital
2012
Hospital Universitari Germans Trias i Pujol
2011
Little is known about the difference in gene expression between carcinoma‐associated fibroblasts (CAFs) and paired normal colonic (NCFs) colorectal cancer. Paired CAFs NCFs were isolated from eight primary human carcinoma specimens. In culture conditions, soluble factors secreted by conditioned media increased clonogenicity migration of epithelial cancer cells lines to a greater extent than did NCF. vivo, more competent as tumour growth enhancers when co‐inoculated with cell lines. Gene...
Epithelial ovarian cancer (EOC) is the fifth leading cause of death in women diagnosed with gynecologic malignancies. The low survival rate because its advanced-stage diagnosis and either intrinsic or acquired resistance to standard platinum-based chemotherapy. So, development effective innovative therapeutic strategies overcome cisplatin remains a high priority.To investigate new treatments vivo models reproducing EOCs tumor growth, we generated preclinical model after orthotopic...
The importance of tumor microenvironment (TME) as a relevant contributor to cancer progression and its role in the development de novo resistance targeted therapies has become increasingly apparent. However, mechanisms microenvironment-mediated drug for nonspecific conventional chemotherapeutic agents, such platinum compounds or antimetabolites, are still unclear.Here we describe mechanism induced by soluble factors released carcinoma-associated fibroblasts (CAFs) that induce translocation...
Abstract Purpose: The aim of the study is blocking recruitment a protective stroma by altering crosstalk between normal stromal cells and tumor for stripping tumors protection conferred microenvironment. Experimental Design: A transcriptomic analysis cocultured colonic fibroblasts colorectal was performed. We focused on molecules that mediate communication both compartments entail fibroblasts’ activation alteration sensitivity to chemotherapy. identified targets tumor–stroma interaction....
// Mireia Berdiel-Acer 1 , Antoni Berenguer 2 Rebeca Sanz-Pamplona Daniel Cuadras Xavier Sanjuan 3 Maria José Paules Cristina Santos 4 Ramon Salazar Victor Moreno Gabriel Capella 5 Alberto Villanueva David G. Molleví Translational Research Laboratory, Catalan Institute of Oncology, IDIBELL, L'Hospitalet de Llobregat, Catalonia, Spain Biomarkers and Susceptibility Unit, Cancer Prevention Monitoring Programme, Pathology Department, Hospital Universitari Bellvitge, Medical Oncology Hereditary...
Abstract HER3 is highly expressed in luminal breast cancer subtypes. Its activation by NRG1 promotes of AKT and ERK1/2, contributing to tumour progression therapy resistance. HER3-targeting agents that block this activation, are currently under phase 1/2 clinical studies, although they have shown favorable tolerability, their activity as a single agent has proven be limited. Here we show phosphorylation cells occurs paracrine manner mediated cancer-associated fibroblasts (CAFs). Moreover,...
Carcinoma-associated fibroblasts (CAFs) are important contributors of microenvironment in determining the tumor's fate. This study aimed to compare influence liver and primary tumor on behavior colorectal carcinoma. Conditioned medium (CM) from normal colonic (NCFs), CAFs (CAF-PT) or metastasis (CAF-LM) were obtained. We performed functional assays test each CM cell lines. Microarray gene set enrichment analysis (GSEA) DLD1 cells cultured matched CM. In cells, CAF-LM compared with CAF-PT NCF...
The differential gene expression patterns between normal colonic fibroblasts (NCF), carcinoma-associated from primary tumors (CAF-PT), and CAFs hepatic metastasis (CAF-LM) are hypothesized to be useful for predicting relapse in tumors. A transcriptomic profile of NCF (n = 9), CAF-PT 14), CAF-LM 11) was derived. Prediction Analysis Microarrays (PAM) used obtain molecular details each fibroblast class, differentially expressed transcripts were classify patients according recurrence status....
Chemotherapy (CTX) remains the standard of care for most aggressive tumours, including breast cancer (BC). In BC chemotherapeutic regimens, maximum tolerated dose cytotoxic drugs is administered at regular intervals, and cells can re-grow or adapt during resting periods between cycles. The impact tumour microenvironment on fate after CTX poorly understood. Here, we show that paracrine signalling from CTX-treated to stromal fibroblasts drive cell recovery drug withdrawal. Interferon β1...
Targeted therapies have shown striking success in the treatment of cancer over last years. However, their specific effects on an individual tumor appear to be varying and difficult predict. Using integrative modeling approach that combines mechanistic regression modeling, we gained insights into response mechanisms breast cells due different ligand–drug combinations. The multi-pathway model, capturing ERBB receptor signaling as well downstream MAPK PI3K pathways was calibrated time-resolved...
We isolate and culture carcinoma-associated fibroblasts (CAFs) from primary tumour (CAFpt), CAFs corresponding synchronous liver metastasis (CAFlm) as well normal colonic (NCF) the same patient. From these cultures, conditioned media (CM) was obtained. Culture of a wide panel colorectal pancreatic cell lines in CM CAFlm resulted overexpression mRNA PRL-3 higher than non-activated fibroblasts. Moreover expression correlates with alpha-SMA deposition collagen fibrils stroma. demonstrate that...
Abstract HER3 is highly expressed in luminal breast cancer subtypes. Its activation by NRG1 promotes of AKT and ERK1/2, contributing to tumour progression therapy resistance. HER3-targeting agents that block this activation, are currently under phase 1/2 clinical studies, although they have shown favorable tolerability, their activity as a single agent has proven be limited. Here we show phosphorylation cells occurs paracrine manner mediated cancer-associated fibroblasts (CAFs). Moreover,...
2037 Background: MGMT (O6-methyl-DNA-methyltransferase) gene promoter methylation and tissue expression predict benefit from alkylating chemotherapy in GB. Tumoral can be detected patients’ serum. We studied the concordance of pattern (t-MGMT+ or -) with paired MSP (methylation specific polymerase-chain reaction) serum DNA (s-MGMT+ protein measured by quantitative immunohistochemistry (q-IHC) a series GBs complete clinical follow-up. Methods: was performed 70 patient samples 37 patient's...
8095 Background: Pemetrexed is approved as second-line therapy in advanced NSCLC. In this study, we evaluate the efficacy and safety of pemetrexed patients with Advanced NSCLC previously treated also perform a pharmacogenomic analysis to determine if biologic characteristics could be related any clinical benefit. Methods: 195 stage IIIB (malignant pleural effusion) or IV were selected receive (500 mg/m2, infusion) supplemented vitamin B12, folic acid dexamethasone prophylaxis. DNA was...
<p>PDF file, 282K, Presence of aberrant mitotic figures in engrafted OVA1X tumors analyzed by co-immunostaining with anti-H3S10ph, anti-tubulin. Chromosomes were labeled DAPI.</p>
<p>Supplementary Figure 5</p>
<p>Supplementary Figure 1</p>
<p>Supplementary Figure 2</p>
<p>Supplementary Figure 6 A and B</p>
<p>Supplementary Figure 4</p>
<p>PDF file, 282K, Presence of aberrant mitotic figures in engrafted OVA1X tumors analyzed by co-immunostaining with anti-H3S10ph, anti-tubulin. Chromosomes were labeled DAPI.</p>