A5007169400- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Breast Cancer Treatment Studies
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- HER2/EGFR in Cancer Research
- Estrogen and related hormone effects
- Advanced Breast Cancer Therapies
- Global Cancer Incidence and Screening
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Cancer Treatment and Pharmacology
- Cancer Cells and Metastasis
- Nuclear Receptors and Signaling
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Epigenetics and DNA Methylation
- Glutathione Transferases and Polymorphisms
- RNA modifications and cancer
- RNA Research and Splicing
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related Molecular Pathways
- Circular RNAs in diseases
- Ovarian cancer diagnosis and treatment
- Metabolism, Diabetes, and Cancer
Martin Luther University Halle-Wittenberg
2016-2025
University Hospital in Halle
2010-2024
Primary Children's Hospital
2024
Luther University
2022-2023
Klinik und Poliklinik für Urologie
2023
Addis Ababa University
2023
University of Hildesheim
2022
University of Gezira
2017
Weill Cornell Medical College in Qatar
2017
Wittenberg University
2015
The tumor-suppressive let-7 microRNA family targets various oncogene-encoding mRNAs. We identify the HMGA2, LIN28B and IGF2BP1 to form a antagonizing self-promoting oncogenic triangle. Surprisingly, 3′-end processing of mRNAs is unaltered in aggressive cancers tumor-derived cells although synthesis was proposed escape attack by APA-dependent (alternative polyadenylation) 3′ UTR shortening. However, expression triangle factors inversely correlated with levels promoted impairing biogenesis....
Breast cancer tumors are known to be highly heterogeneous and differences in their metabolic phenotypes, especially at protein level, less well-understood. Profiling of metabolism-related proteins harbors the potential establish new patient stratification regimes biomarkers promoting individualized therapy. In our study, we aimed examine relationship between metabolism-associated expression profiles clinicopathological characteristics a large cohort breast patients. specimens from 801...
Phosphatidylinositide-3-kinase (PI3K) regulates proliferation and apoptosis; somatic PIK3CA-mutations may activate these processes. Aim of this study was to determine the prevalence in a cohort early stage breast cancer patients association course disease.From an unselected 1270 (PiA, Prognostic Assessment routine application, NCT01592825) 1123 tumours were tested for three PIK3CA hotspot-mutations H1047R, E545K, E542K by qPCR. Primary objectives their tumour characteristics. Secondary...
Abstract The Hippo/YAP1 signaling pathway regulates normal development by controlling contact inhibition of growth. In cancer, YAP1 activation is often dysregulated, leading to excessive tumor growth and metastasis. SRC kinase can cross talk Hippo disrupting adherens junctions, repressing the cascade, or activating promote proliferation. Here, we demonstrate that IGF2 messenger RNA-binding protein 1 (IGF2BP1) impedes repression in carcinomas. IGF2BP1 stabilizes RNA (mRNA) enhances synthesis...
Abstract GRB2 is an adaptor protein of HER2 (and several other tyrosine kinases), which we identified as a novel BECN1 (Beclin 1) interacting partner. co-immunoprecipitated with in breast cancer cell lines and regulates autophagy through mechanism involving the modulation class III PI3Kinase VPS34 activity. In ovo studies CAM (Chicken Chorioallantoic Membrane) model indicated that knockdown, well overexpression loss-of-function mutants (Y52A S86A-R88A) compromised tumor growth. These...
Epithelial-to-mesenchymal transition (EMT) is a hallmark of aggressive, mesenchymal-like high-grade serous ovarian carcinoma (HGSOC). The SRC kinase key driver cancer-associated EMT promoting adherens junction (AJ) disassembly by phosphorylation-driven internalization and degradation AJ proteins. Here, we show that the IGF2 mRNA-binding protein 1 (IGF2BP1) up-regulated in HGSOC promotes activation previously unknown protein-ligand-induced, but RNA-independent mechanism. IGF2BP1-driven...
Today, more than 70% of patients with primary node-negative breast cancer are cured by local therapy alone. Many receive overtreatment adjuvant chemotherapy due to inadequate risk assessment. So far, few clinical trials have prospectively evaluated tumor biology based prognostic factors. Risk assessment a biological algorithm including invasion factors urokinase-type plasminogen activator (uPA) and its inhibitor type 1 (PAI-1) will assess up 35-55% as low-risk thus avoid chemotherapy. In...
In contrast with breast cancers (BCs) in other parts of the world, most previous studies reported that majority BCs sub-Saharan Africa are estrogen-receptor (ER) negative. However, a recent study using US SEER database showed proportion ER-negative BC is comparable between US-born blacks and West-African born but substantially lower East African-born blacks, over 74% patients Ethiopians or Eritreans. this paper, we provide first report on Ethiopia, relation to progesterone-receptor (PgR)...
The aim was to evaluate the accuracy of BI-RADS categories 3 - 5 in breast ultrasound (US) as first-line imaging method.5077 examinations a consecutive, unselected and mixed collective symptomatic asymptomatic patients were performed. Of these examinations, 835 cases BIRADS could be analyzed.The PPV with respect malignant lesion for 3, 4, 0.03, 0.48, 0.97, respectively. When 4 are considered suspicious, ratio benign findings corresponds 1:1.8. Analyzing lesions, sensitivity, specificity...
The effect of endogenous parathyroid hormone-related protein (PTHrP) on gene expression in breast cancer cells was studied. We suppressed PTHrP MDA-MB-231 by RNA interference and analyzed changes microarray analysis. More than 200 genes showed altered response to a PTHrP-specific small interfering (si) (siPTHrP). Cell cycle-regulating CDC2 (CDC25B Tome-1) that control activity increased the presence siPTHrP. also found be higher siPTHrP-treated cells. Studies with peptides 1-34 67-86,...
Breast cancer is the most common in women worldwide. The tumor microenvironment contributes to progression by inducing cell dissemination from primary and metastasis. TGF β signaling involved breast specifically elevated during metastatic transformation aggressive cancer. In this study, we performed genomewide correlation analysis of TGFBR 2 expression a panel 51 lines identified that MET coregulated with . This was confirmed at protein level human tissues. Flow cytometric luminal basal‐like...
Uncontrolled proliferation and altered metabolic reprogramming are hallmarks of cancer. Active glycolysis glutaminolysis characteristic features these required for tumorigenesis. A fine balance between cancer metabolism autophagy is a prerequisite homeostasis within cells. Here we show that glutamate pyruvate transaminase 2 (GPT2), which serves as pivot glutaminolysis, highly upregulated in aggressive breast cancers, particularly the triple-negative subtype. Abrogation this enzyme results...
Abstract HER3 is highly expressed in luminal breast cancer subtypes. Its activation by NRG1 promotes of AKT and ERK1/2, contributing to tumour progression therapy resistance. HER3-targeting agents that block this activation, are currently under phase 1/2 clinical studies, although they have shown favorable tolerability, their activity as a single agent has proven be limited. Here we show phosphorylation cells occurs paracrine manner mediated cancer-associated fibroblasts (CAFs). Moreover,...
Abstract The low overall survival rates of patients with breast cancer in sub-Saharan Africa (SSA) are driven by regionally differing tumor biology, advanced stages at diagnosis, and limited access to therapy. However, it is not known whether regional differences the composition microenvironment (TME) exist affect patients’ prognosis. In this international, multicentre cohort study, 1,237 formalin-fixed, paraffin-embedded samples, including samples “African Breast Cancer-Disparities Outcomes...
Abstract Rho GDP dissociation inhibitor β (Rho-GDIβ), an of GTPases, is primarily expressed by hematopoietic cells but also found in epithelial cancer cells. Recently, we have identified Rho-GDIβ as a target the transcription factor Ets1. Here, show that, breast cells, Ets1 regulates expression and binds to upstream region gene. Furthermore, primary cancer, coexpressed with Studying function that Rho-GDIβ–specific small interfering RNA increased cellular migration decreased cyclooxygenase-2...
<b><i>Introduction:</i></b> Triple-negative breast cancer (TNBC) is considered the most aggressive type of (BC) with limited options for therapy. TNBC a heterogeneous disease and tumors have been classified into subtypes using gene expression profiling to distinguish basal-like 1, 2, immunomodulatory, mesenchymal, mesenchymal stem-like, luminal androgen receptor (LAR), one nonclassifiable group (called unstable). <b><i>Objectives:</i></b> The...
Breast cancer (BC) is the leading cause of mortality among women in Ethiopia. Overall, African ancestry have highest death toll due to BC compared other racial/ethnic groups. The disparity unclear. Recently, studies conducted United States and high-income countries highlighted role microbial dysbiosis initiation, tumor growth, treatment outcome. However, extent which inter-individual differences makeup microbiota are associated with clinical histopathological outcomes Ethiopian has not been...
Endocrine therapy for breast cancer (BC) patients is highly underutilized in rural Ethiopia and other African countries.This study aims to assess the feasibility of adherence tamoxifen Ethiopia.We ascertained hormone receptor (HR) status 101 women diagnosed with BC from January 2010 December 2015 who had surgery Aira Hospital, Ethiopia. From 2013, was offered HR-positive (HR+) tumors. Prescription refill records a structured questionnaire were used receipt tamoxifen.Of tested HR during...
In intermediate risk hormone receptor (HR) positive, HER2 negative breast cancer (BC), the decision regarding adjuvant chemotherapy might be facilitated by multigene expression tests. all, 142 BCs were investigated using PAM50-based test Prosigna® in a prospective multicentric study. 119/142 cases, molecular subtyping was compared with local and two central (C1 C6) molecular-like subtypes relying on both immunohistochemistry (IHC; HRs, HER2, Ki-67) IHC + tumor grade (IHC+G) subtyping....
The recent development of multi-gene assays for gene expression profiling has contributed significantly to the understanding clinically and biologically heterogeneous breast cancer (BC) disease. PAM50 is one these used stratify BC patients individualize treatment. present study was conducted characterize PAM50-based intrinsic subtypes among Ethiopian patients.Formalin-fixed paraffin-embedded tissues were collected from 334 who attended five different health facilities. All samples assessed...
Purpose The objective of this study was to compare tumor characteristics, biomarkers, and surrogate subtypes breast cancer between Sudanese German women. Methods Tumor characteristics immunohistochemistry markers (estrogen receptor [ER], progesterone [PR], human epidermal growth factor 2 [HER2]) were collected from the routine assessment consecutive patients with invasive diagnosed 2010 2015 (Gezira University Pathology Laboratory, Gezira, Sudan) 1999 2013 (Breast Centre,...